Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : November | Volume : 11 | Issue : 11 | Page : WC01 - WC04

IL-8 mRNA and Serum Levels in Vitiligo Patients: A Case Control Study

Azza Gaber Farag, Alaa Hassan Marae, Mona Salah Eldin Habib, Reda Zakaria Mahfouz, Nada Farag Elnaidany, SalwaIbrahim Youssef, Sherifa Morsy El-Feky

1. Assistant Professor, Department of Dermatology, Andrology and STDs, Faculty of Medicine, Menoufia University, Al Minufiyah, Egypt. 2. Professor, Department of Dermatology, Andrology and STDs, Faculty of Medicine, Menoufia University, Al Minufiyah, Egypt. 3. Lecturer, Department of Medical Biochemistry, Faculty of Medicine, Menoufia University, Al Minufiyah, Egypt. 4. Lecturer, Department of Clinical Pathology, Menoufia University, Shebin Elkom, Egypt; Cleveland Clinic, Lerner College of Medicine (CCLCM), CWR University, Cleveland, OH, USA. 5. Assistant Professor, Department of Clinical Pharmacy, Faculty of Pharmacy-MSA University, October City, Egypt. 6. Lecturer, Department of Medical Microbiology and Immunology, October 6 University, Egypt. 7. Resident Doctor, Department of Dermatology, Ministry of Health, Egypt.

Correspondence Address :
Dr. Sherifa Morsy El-Feky,
Resident Doctor, Department of Dermatology, Ministry of Health, Egypt.
E-mail: elfekysherifa@yahoo.com

Abstract

Introduction: Many theories are suggested to explain aetiology of vitiligo, such as autoimmune, genetic and biochemical. Interleukin-8 (IL-8) is a pro-inflammatory chemokine which is evaluated in the pathogenesis of some skin diseases, like psoriasis, however, in vitiligo, few studies were reported regarding this issue.

Aim: The aim of the present study was to investigate serum level of IL-8 and IL-8 mRNA in patients with different types of vitiligo to validate its role in aetiopathogenesis of vitiligo.

Materials and Methods: This case-control study was conducted on 39 vitiligo patients and 15 age and gender matched healthy controls. The participants were selected from Dermatology Outpatient Clinic, Menoufia University Hospitals from October 2014 to October 2015. They were subjected to clinical history, examination and full general examination. Detailed dermatological examination including VASI score was applied. IL-8 serum level was measured by ELISA and IL-8 mRNA was quantitated by Real Time-PCR. The statistical analysis was done using SPSS, version 20.0. Mann Whitney U test was used to compare two quantitative not normally distributed. Chi-square test (?2) was used to study association between two qualitative variables. Spearman correlation test was applied to assess correlation between two continuous quantitative variables. A p=0.05 was considered significant.

Results: IL-8 serum level and mRNA concentration mean values were significantly elevated in vitiligo patients (26.2543.28 pg/mL and 8.4811.92 ng/mL) than controls (0.570.50 pg/mL and 0.600.32 ng/mL) (p=0.002, for both), and they showed significant positive correlation with each other (r=0.622; p=0.001). Moreover, both of them revealed significant high values in localised vitiligo (62.2074.39 pg/mL and 12.4213.85 ng/mL) than generalised (18.3929.49 pg/mL and 7.6111.52 ng/mL) (p=0.000 and p=0.004), respectively.

Conclusion: Serum IL-8 chemokine and its mRNA increased significantly in vitiligo patients indicating that it may have a dynamic role in vitiligo development and participate in its pathogenesis.

Keywords

ELISA, IL-8 mRNA, Pathogenesis, Real time PCR

How to cite this article :

Azza Gaber Farag, Alaa Hassan Marae, Mona Salah Eldin Habib, Reda Zakaria Mahfouz, Nada Farag Elnaidany, SalwaIbrahim Youssef, et al.. IL-8 MRNA AND SERUM LEVELS IN VITILIGO PATIENTS: A CASE CONTROL STUDY. Journal of Clinical and Diagnostic Research [serial online] 2017 November [cited: 2018 Jan 20 ]; 11:WC01-WC04. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=November&volume=11&issue=11&page=WC01-WC04&id=10857

DOI and Others

DOI: 10.7860/JCDR/2017/30392.10857

Date of Submission: May 26, 2017
Date of Peer Review: Aug 05, 2017
Date of Acceptance: Aug 31, 2017
Date of Publishing: Nov 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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