Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : November | Volume : 11 | Issue : 11 | Page : BC15 - BC18

Study of Thalassemia and Haemoglobinopathies in Pokhara, Nepal

Ganesh Bastola, Rishikeshav Acharya, Niraj Dhakal, Umesh Prasad Gupta

1. Lecturer, Regional College of Health Science and Technology, Nayabazaar, Pokhara, Nepal. 2. Medical Technologist, Department of Laboratory Science, Life Care Diagnostics and Research Center, Pokhara Pvt. Ltd, Pokhara, Nepal. 3. Laboratory Chief, Department of Laboratory Science, NRL, Pokhara Pvt. Ltd. Pokhara, Nepal. 4. Lecturer, Department of Science and Technology, School of Health and Allied Sciences, Pokhara, Nepal.

Correspondence Address :
Mr. Ganesh Bastola,
Lecturer, Regional College of Health Science and Technology, Nayabazaar, Pokhara-33700, Nepal.


Introduction: Presence of thalassemias and haemogl-obinopathies are described in some communities especially from Terai belt. However, there are no any population based studies done till date, including other parts of Nepal. So, this study may indicate the presence of haemoglobin disorder in other parts of Nepal as well.

Aim: This study was done to know the presence of Haemoglobin disorder in this part of Nepal. In addition, this study also focuses on the application of electrophoresis and densitometric quantification for easy and accurate determination of Haemoglobin disorder.

Materials and Methods: In this cross-sectional study, a total of 45 blood samples of all age groups with mean corpuscular volume and mean corpuscular Haemoglobin less than 80 femtoliter (fL) and 27 picogram (pg) respectively, were analysed for Haemoglobin pattern and their quantification. The Haemoglobin electrophoresis and quantification of haemoglobin was performed by using automated Haemoglobin electrophoresis system (Genio S, Italy) in an alkaline medium.

Results: Out of total 45 subjects, 42 subjects (93%) showed normal Haemoglobin patterns. Two subjects were suspected to have beta thalassaemia trait based on the Haemoglobin A2 (HbA2) level. One subject showed the heterozygote Haemoglobin E. In addition, automated haemoglobin electrophoresis and direct densitometric quantification is easy and accurate method for determining haemoglobin pattern rather than manual process.

Conclusion: Haemoglobin disorders are also present in other parts of Nepal, like Pokhara. In addition, this quantitative method could be of great diagnostic value rather than classical comparative method.


Electrophoresis, Haemoglobin, Haemoglobin disorder

How to cite this article :

Ganesh Bastola, Rishikeshav Acharya, Niraj Dhakal, Umesh Prasad Gupta. STUDY OF THALASSEMIA AND HAEMOGLOBINOPATHIES IN POKHARA, NEPAL. Journal of Clinical and Diagnostic Research [serial online] 2017 November [cited: 2018 Jan 20 ]; 11:BC15-BC18. Available from

DOI and Others

DOI: 10.7860/JCDR/2017/29845.10916


Date of Submission: May 07, 2017
Date of Peer Review: Jun 21, 2017
Date of Acceptance: Aug 31, 2017
Date of Publishing: Nov 01, 2017

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