Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : October | Volume : 11 | Issue : 10 | Page : ZC17 - ZC19

Mast Cell Density in Oral Lesions using Metachromatic Stains: A Comparative Study

Shilpa Chirappurath Natesan, Jose George, Rekha Krishna Pillai, Bindhu Pushparajan Ramakrishnan, Priya Thomas

1. General Practitioner, Mother Mary Dental Clinic, Ernakulam, Kerala, India. 2. General Practitioner, Punnapuzha Dental Care, Ernakulam, Kerala, India. 3. Head and Professor, Department of Oral Pathology and Microbiology, Annoor Dental College and Hospital, Ernakulam, Kerala, India. 4. Professor, Department of Oral Pathology and Microbiology, Annoor Dental College and Hospital, Ernakulam, Kerala, India. 5. Reader, Department of Oral Pathology and Microbiology, Annoor Dental College and Hospital, Ernakulam, Kerala, India.

Correspondence Address :
Dr. Shilpa Chirappurath Natesan,
Aiswarya, Mannam P O, North Paravur-683520, Ernakulam, Kerala, India.
E-mail: sshilpacn@gmail.com

Abstract

Introduction: Mast Cells (MCs) are bone marrow derived granular cells, distributed throughout the body near blood vessels, nerves and subepithelial areas. MC granules stain by basic dyes but are most readily demonstrated by metachromatic dyes such as toluidine blue and azure A.

Aim: This study focuses on evaluating and comparing the count of MCs by identification and staining of these cells by azure A with toluidine blue as a control, in normal oral mucosa and in various other oral pathologies.

Materials and Methods: Five cases each of Normal Oral Mucosa (NOM), Inflammatory Fibrous Hyperplasia (IFH), Oral Pyogenic Granuloma (OPG), Oral Lichen Planus (OLP) and Oral Squamous Cell Carcinoma (OSCC) were stained with 1% toluidine blue and azure A.

Results: Mean MC count was higher in all four lesions when compared to normal oral mucosa with both stains. OLP exhibited the maximum amount of mean MC count when compared to other pathologies under study. With all four lesions, toluidine blue exhibited higher number of MC density (count/high power field) compared to azure A.

Conclusion: Higher count of MCs was noticed in all four lesions indicating a possible role of MCs in their pathogenesis either directly or indirectly. Also, the number of degranulated MCs was more in OLP followed by OSCC, IFH and OPG.

Keywords

Azure A, Oral cancer, Oral lichen planus, Oral pyogenic granuloma, Toluidine blue

How to cite this article :

Shilpa Chirappurath Natesan, Jose George, Rekha Krishna Pillai, Bindhu Pushparajan Ramakrishnan, Priya Thomas. MAST CELL DENSITY IN ORAL LESIONS USING METACHROMATIC STAINS: A COMPARATIVE STUDY. Journal of Clinical and Diagnostic Research [serial online] 2017 October [cited: 2018 Jan 23 ]; 11:ZC17-ZC19. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=October&volume=11&issue=10&page=ZC17-ZC19&id=10744

DOI and Others

DOI: 10.7860/JCDR/2017/30460.10744

Date of Submission: May 27, 2017
Date of Peer Review: Jul 19, 2017
Date of Acceptance: Sep 07, 2017
Date of Publishing: Oct 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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