Micronucleus Assay in Urothelial Cells in Cancer CervixCorrespondence Address :
Dr. Suresh Kumar Sundararajan,
C2, 402, Aksahya homes, Nandivaram, Guduvanchery, Chennai-603202, Tamil Nadu, India.
Introduction: Cancer ranks third among the ten leading global causes of death. To evaluate the genotoxic risks, observed as DNA damages, can be assessed by Micronucleus (MN) test.
Aim: To identify the occurrence of MN in normal and cancer cervix and find the correlation between MN and stage of cancer.
Materials and Methods: A total of 60 females were included in the study and visual examination of the cervix was done. Based on the examination two groups were formed: A- Normal cervix (n-23) and B- Presence of erosion or growth or ulcer etc., in cervix (n-37). Midstream urine sample was collected and centrifuged from the cases after getting the informed consent. Slides were prepared from the pellet, were fixed in methanol, glacial acetic acid fixative and stained with Giemsa and May Grunwald stain. Statistical analysis was done by student’s t-test and chi-square test.
Results: A linear association was noted between the mean MN count and cancer cervix stage. Almost 18.2% of the Group A cases had significant MN count. Sensitivity and specificity of MN count in Group A was 83.8% and 82.6% respectively. The efficiency was 83.3%.
Conclusion: A statistically significant MN count was seen in the different stages of cancer cervix. There are cases who had normal findings on visual inspection of cervix but with significant MN count are prone for malignant transformation. MN assay is an easy, non-invasive, cost-effective method and can be used as a screening test for a large population.
Cervical malignancy, Genotoxicity, Nuclear abnormalities
Suresh Kumar Sundararajan, Pratheepa Sivasankari Natarajan, Kanchana. MICRONUCLEUS ASSAY IN UROTHELIAL
CELLS IN CANCER CERVIX. Journal of Clinical and Diagnostic Research [serial online] 2017 March [cited: 2017 May 28 ]; 11:XC01-XC03. Available from
Date of Submission: Aug 09, 2016
Date of Peer Review: Nov 28, 2016
Date of Acceptance: Jan 20, 2017
Date of Publishing: Mar 01, 2017
Financial OR OTHER COMPETING INTERESTS: None.
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