Correlation of Plasma Neutrophil Elastase Activity and Endogenous Protease Inhibitor Levels with the Severity of Pre-eclampsiaCorrespondence Address :
Dr. AV Moideen Kutty,
Registrar, Professor, Sri Devaraj Urs Academy of Higher Education and Research,
Tamaka, Kolar-563103, Karnataka, India.
Introduction: Pre-eclampsia (PE) is a common maternal syndrome characterized by severe systemic inflammatory response including neutrophil activation leading to uncontrolled activity of elastase. The excessive activity of elastase would lead to destroyal of the integrity of endothelial cells and could exacerbate the pathophysiological symptoms in PE. Thus, assessment of NE activity and its control mechanisms would be of relevance in the determination of severity of PE.
Aim: To correlate the activity of plasma NE and its endogenous inhibitors a1-antitrypsin (a1-AT) and a2-macroglobulin (a2-MG) with severity of PE.
Materials and Methods: A comparative study was conducted between normotensive pregnant (n=50) and pre-eclamptic (n=50) women. Serum C-Reactive Protein (CRP) was estimated by rapid latex slide and uric acid by uricase method. Plasma elastase was estimated using succinyl tri- L-alanyl-p-nitroanilide as substrate. Plasma a1-AT, a2-MG and NE- a1-AT complex were quantified by ELISA. ANOVA and Pearson’s correlation tests were used to analyze the data. The results were expressed as mean±SD and p-value <0.001 was considered statistically highly significant.
Results: The activity of elastase was increased significantly in severe PE (0.62±0.08) in comparison to normal (0.35±0.10) and mild pre-eclamptic subjects (0.37±0.03). The values of a1-AT were significantly less in mild (83.94±25.08) and severe PE (68.58+26.39) in comparison to normal (110.26±42.39). There was a significant rise in the levels of a2-MG in severe PE. However, the complex estimation did not evince any significant changes.
Conclusion: The results of the present study indicate a significantly elevated elastase activity, a2-MG levels and decreased a1-AT in severe PE patients. The correlation analyses of PE severity parameters with NE, a1-AT and a2-MG further support the roles of these molecules in the assessment of severity of PE.
a1-antitrypsin, a2-macroglobulin, NE- a1-AT complex
Mamatha Kunder, AV Moideen Kutty, V Lakshmaiah, SR Sheela. CORRELATION OF PLASMA NEUTROPHIL
ELASTASE ACTIVITY AND ENDOGENOUS
PROTEASE INHIBITOR LEVELS WITH THE
SEVERITY OF PRE-ECLAMPSIA. Journal of Clinical and Diagnostic Research [serial online] 2017 March [cited: 2017 Mar 29 ]; 11:BC09-BC12. Available from
Date of Submission: Sep 15, 2016
Date of Peer Review: Nov 05, 2016
Date of Acceptance: Dec 01, 2016
Date of Publishing: Mar 01, 2017
Financial OR OTHER COMPETING INTERESTS: None.
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