Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

Users Online : 12178

Original article / research
Year : 2018 | Month : June | Volume : 12 | Issue : 6 | Page : DC27 - DC30

Biofilm Formation in Clinical Isolates of S. aureus is Associated with Presence of Device and Dissemination of Infection

Ana Paula Becker, Cicero AG Dias, Alexandre José Macedo

1. Professor, Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Brazil. 2. Phd, Departamento de Microbiologia e Parasitologia, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Brazil. 3. Professor, Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Brazil.

Correspondence Address :
Dr. Ana Paula Becker,
Avenida Ipiranga, 2752 sala 705, Porto Alegre, Rio Grande do Sul, CEP: 90610-000 Country, Brazil.
E-mail: anapbecker1@gmail.com

Abstract

Introduction: Biofilms are complex microbial communities attached to abiotic or biotic surfaces. These communities produce their own extracellular matrix, where they interact with one another and with the environment.

Aim: To observe the biofilm formation isolates of Staphylococcus aureus from South Brazil.

Materials and Methods: A total of 126 consecutive S. aureus isolates were collected, causing a variety of infections at a tertiary hospital from 2011 to 2014. We investigated biofilm-forming ability by using a microtiter plate assay (crystal violet method) and compared the clinical characteristics and outcomes of infected patients with biofilm-forming ability. The following clinical characteristics were evaluated: presence of polymicrobial infection; presence of another micro-organism (in another clinical material at the same time); recurrence of infection; presence of device and site of infection.

Results: Biofilm forming bacteria were categorized as high producers (n=46, 36.5%), moderate producers (n=59, 46.8%) and weak producers or non-producers (n=21, 16.7%). The presence of another microorganism isolated in the same day in another clinical specimen was significantly associated with biofilm-formation (p<0.006) as well the presence of invasive devices (p<0.02).

Conclusion: This study allows planning medical conducts, e.g., the choice of appropriate antimicrobials, in patients with devices such as catheters and patients with infections at different sites to adequately adjust treatment of infections by biofilm-forming bacteria.

Keywords

Bacterial dissemination, Biofilm production, Hospital infection, Medical device

How to cite this article :

Ana Paula Becker, Cicero AG Dias, Alexandre José Macedo. BIOFILM FORMATION IN CLINICAL ISOLATES OF S. AUREUS IS ASSOCIATED WITH PRESENCE OF DEVICE AND DISSEMINATION OF INFECTION. Journal of Clinical and Diagnostic Research [serial online] 2018 June [cited: 2018 Jun 18 ]; 12:DC27-DC30. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=June&volume=12&issue=6&page=DC27-DC30&id=11632

DOI and Others

DOI: 10.7860/JCDR/2018/35117.11632

Date of Submission: Dec 10, 2017
Date of Peer Review: Feb 16, 2018
Date of Acceptance: May 05, 2018
Date of Publishing: Jun 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: None.

JCDR is now Monthly and more widely Indexed .
  • Emerging Sources Citation Index (Web of Science, thomsonreuters)
  • Index Copernicus ICV 2016: 132.37
  • SCOPUS
  • Academic Search Complete Database
  • Directory of Open Access Journals (DOAJ)
  • EBSCOhost
  • Embase & EMbiology
  • Google Scholar
  • HINARI Access to Research in Health Programme
  • Indian Science Abstracts (ISA)
  • Journal seek Database
  • Google
  • Popline (reproductive health literature)
  • www.omnimedicalsearch.com