Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : August | Volume : 12 | Issue : 8 | Page : BC16 - BC18

A study on Association of Antioxidant Status of Red Blood Cells with Type 2 Diabetes

Shabari Mangalore Shenoy, Ullal Harshini Devi, Suchetha N Kumari, Chethan Subramanya

1. Intern, Department of Biochemistry, K.S. Hegde Medical Academy Mangalore, Karnataka, India. 2. Assistant Professor, Department of Biotechnology Engine ring, NMAMIT Nitte, Karkala, Karnataka, India. 3. Professor, Department of Biochemistry, K.S. Hegde Medical Academy Mangalore, Karnataka, India. 4. Assistant Professor, Department of General Medicine, K.S. Hegde Medical Academy Mangalore, Karnataka, India.

Correspondence Address :
Dr. Shabari Mangalore Shenoy,
Brindavan, Court Road, Puttur 574201 Karnataka, Puttur, Karnataka, India.
E-mail: shabarishenoy94@gmail.com

Abstract

Introduction: Oxidative stress plays a role in the pathogenesis of type 2 Diabetes either by effecting insulin secretion or increasing resistance to insulin. Oxidative stress is dealt by the body with the help of several antioxidant systems. The antioxidant levels in disorders causing oxidative stress such as diabetes mellitus are found to be low. Total Antioxidant Capacity (TAC), Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) levels are found to be depleted in diabetic groups when compared to control group comprising healthy individuals. The activities of SOD and GPx are significantly low in diabetic patients.

Aim: To determine the total antioxidant capacity and levels of antioxidants such as SOD and GPx in patients with type 2 diabetes and association between antioxidant levels and type 2 diabetics as well as type 2 diabetes and its associated complications.

Materials and Methods: The study population comprised of 150 individuals, 50 subjects belonging to each subgroup of type 2 diabetics, type 2 diabetics and its associated complications and healthy subjects. Study was conducted in a tertiary care centre in Mangalore, Karnataka during a study period of September to December 2016. Total antioxidant capacity of RBC, fasting blood levels of SOD and GPx were estimated by phosphomolybdenum method, NBT reduction method and Rotruck method respectively.

Results: The results of our study showed that total antioxidant capacity, SOD and GPx were lower among diabetics and diabetic patients with complications as compared to normal non-diabetic subjects. No significant differences in oxidative stress were found between patients with and without chronic complications of diabetes.

Conclusion: It is evident from the results of our study that there exists significant deficiency of markers of antioxidant defence in diabetics as well as diabetics with its associated complications. This indicates that there is a scope for antioxidant supplementation in individuals at risk of developing diabetes in general population and diabetic patients at risk of developing its chronic complications.

Keywords

Antioxidant defence, Glutathione peroxidase, Oxidative stress, Superoxide dismutase

How to cite this article :

Shabari Mangalore Shenoy, Ullal Harshini Devi, Suchetha N Kumari, Chethan Subramanya. A STUDY ON ASSOCIATION OF ANTIOXIDANT STATUS OF RED BLOOD CELLS WITH TYPE 2 DIABETES. Journal of Clinical and Diagnostic Research [serial online] 2018 August [cited: 2018 Jul 23 ]; 12:BC16-BC18. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=August&volume=12&issue=8&page=BC16-BC18&id=11920

DOI and Others

DOI: 10.7860/JCDR/2018/36006.11920

Date of Submission: Feb 14, 2018
Date of Peer Review: Apr 20, 2018
Date of Acceptance: Jun 05, 2018
Date of Publishing: Aug 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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