Pre-microRNA Gene Polymorphisms and Risk of Cervical Squamous Cell CarcinomaCorrespondence Address :
Dr. Anand Narain Srivastava,
Director Research, Department of Pathology, Era's Lucknow Medical College and Hospital, Sarfarz Ganj,
Lucknow-226003, Uttar Pradesh, India.
Introduction: MicroRNAs (miRNAs) are short (~22 nucleotides) regulatory RNAs that can modulate gene expression and are aberrantly expressed in many diseases, including cancer. It has been suggested that, the presence of single nucleotide polymorphisms in precursor miRNAs (pre-miRNAs) can alter miRNA processing, expression and binding to target mRNA and represents another type of genetic variability, that can contribute to the susceptibility of human cancers.
Aim: The present study investigated the genetic variants in pre-miRNAs (hsa-miRNA-196a2 rs11614913 C/T, hsa-miRNA-499 rs3746444 T/C and hsa-miRNA-146a rs2910164 G/C) for their role in cervical cancer susceptibility.
Materials and Methods: The study comprised 164 controls and 184 patients of cervical cancer. The genotypic frequency of miRNA polymorphisms were determined by using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Logistic regression was used for statistical analysis using SPSS Software version 15.0.
Results: Hsa-miRNA-499 rs3746444 T/C polymorphism showed a statistically significant association with considerable risk for cervical cancer at genotypes (CC, p=0.001, OR=4.801) and variant allele (p<0.001, OR=2.307). MiRNA 146a and miRNA 196a2 polymorphisms showed no association with cervical cancer. However, interaction of miRNA polymorphisms with smoking habit showed higher risk of cervical cancer with miRNA 196a2 polymorphism in patients with smoking but no significant modification in the risk of cervical cancer was seen for other polymorphisms.
Conclusion: The results of the present study demonstrate that, miRNA 499 T/C polymorphism is significantly associated with genetic susceptibility to cervical cancer and may have a role in its pathogenesis.
Apoptosis, Cervical Cancer, Single nucleotide polymorphism
Shruti Srivastava, Shalini Singh, Naseem Fatima, Balraj Mittal, Anand Narain Srivastava. PRE-MICRORNA GENE POLYMORPHISMS AND RISK OF CERVICAL SQUAMOUS CELL CARCINOMA. Journal of Clinical and Diagnostic Research [serial online] 2017 September [cited: 2017 Nov 24 ]; 11:GC01-GC04. Available from
Date of Submission: Nov 15, 2016
Date of Peer Review: Feb 10, 2017
Date of Acceptance: May 30, 2017
Date of Publishing: Sep 01, 2017
FINANCIAL OR OTHER COMPETING INTERESTS: None.
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