Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2016 | Month : May | Volume : 10 | Issue : 5 | Page : ZC63 - ZC65

Effect of Diode Laser on Bacteria Beyond the Apex in Relation to the Size of the Apical Preparation An In-Vitro Study

Sistla Venkata Jyotsna, Raja Vatsavai Chakradhar Raju, Jaya Prakash Patil, Thakur Veerandar Singh, Neha Bhutani, Shekar Kamishetty, Kiran Ghatole

1. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India. 2. Professor and Head, Department of Conservative Dentistry and Endodontics, Sri Balaji Dental College, Moinabad, India. 3. Professor and Head, Department of Conservative Dentistry and Endodontics, PMNM Dental College, Bagalkot, India. 4. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India. 5. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India. 6. Professor, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India. 7. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India.

Correspondence Address :
Dr. Sistla Venkata Jyotsna,
Sri Sai College of Dental Surgery, Kothrepally, Vikarabad, India.
E-mail: jyotsnasistla@gmail.com

Abstract

Introduction: Microorganisms causing periapical infection are usually difficult to eradicate after conventional endodontic treatment or even in retreatment resulting in poor outcomes. So the purpose of the study was to assess whether disinfection of root canal with laser had any effect on bacteria in the periapex region.

Aim: The aim of the present study was to evaluate the effects of a diode laser when activated in root canals with varying apical diameters, on the bacteria present beyond the apex of the teeth.

Materials and Methods: Total 30 intact single rooted teeth were taken and decoronated to standardize the root to a length of 12mm. They were divided into three groups depending on last file size used for instrumentation at apex i.e., size 30, 40 and 50 respectively. The samples were then mounted on test tubes such that roots of teeth were in contact with fresh broth of Escherichia coli (ATCC 25922) and left for incubation. Later a diode laser (Ezlase 940, Biolase) was used for disinfection of root canals of the samples. Following this the bacterial inoculums from each test tube were cultured and CFU were obtained from which the mean log values were obtained. Statistical analysis was done using Kruskal Wallis ANOVA test to compare mean CFU in three groups. Mann-Whitney U test with Bonferroni correction was used to compare inter-group differences.

Results: There was statistically significant difference in mean log values of CFU in all the three study groups. Inter-group comparisons showed that, Group A had significantly lower mean CFUs than Group B and C respectively.

Conclusion: The study showed that intracanal irradiation with diode laser had an effect on the bacteria present beyond the apex, and it was influenced by the size of the apical preparation i.e., smaller apical size led to a greater reduction in the bacterial count.

Keywords

Apical diameter, Apical periodontitis, Escherichia coli, Root canal disinfection

How to cite this article :

Sistla Venkata Jyotsna, Raja Vatsavai Chakradhar Raju, Jaya Prakash Patil, Thakur Veerandar Singh, Neha Bhutani, Shekar Kamishetty, et al.. EFFECT OF DIODE LASER ON BACTERIA BEYOND THE APEX IN RELATION TO THE SIZE OF THE APICAL PREPARATION AN IN-VITRO STUDY. Journal of Clinical and Diagnostic Research [serial online] 2016 May [cited: 2018 Jun 21 ]; 10:ZC63-ZC65. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2016&month=May&volume=10&issue=5&page=ZC63-ZC65&id=7791

DOI and Others

DOI: 10.7860/JCDR/2016/17759.7791


Date of Submission: Nov 09, 2015
Date of Peer Review: Jan 29, 2016
Date of Acceptance: Feb 04, 2016
Date of Publishing: May 01, 2016


Financial OR OTHER COMPETING INTERESTS: None.

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