Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2016 | Month : May | Volume : 10 | Issue : 5 | Page : ZC34 - ZC38

Peri-Implant Sulcus Fluid (PISF) Matrix Metalloproteinase (MMP) -8 Levels in Peri-Implantitis

René Thierbach, Kurtrt Maier, Timo Sorsa, Päivi Mäntylä

1. Department of Dental Medicine - periodontology, German Armed Forces Hospital, Berlin, Germany. 2. Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland; 2) Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital, Helsinki, Finland; 3) Division of Periodontology, Department of Dental Medicine, Karolinska Institute, Huddinge, Sweden 3. DentognosticsGmBH, Jena, Germany 4. Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.

Correspondence Address :
Dr. Päivi Mäntylä,
Department of Oral and Maxillofacial Diseases, University of Helsinki
PO. Box 41, FIN-00014 University of Helsinki, Helsinki, Finland.


Introduction: Matrix Metalloproteinase (MMP) -8 plays crucial role in pathogenesis of periodontitis and is also a possible biomarker candidate in peri-implantitis.

Aim: The aim of the study was to analyse MMP-8 levels in peri-Implant Sulcus Fluid (PISF) from peri-implantitis affected implants in smoking and non-smoking patients with different periodontal health status of natural teeth before and after peri-implantitis treatment.

Settings and Design: Altogether 29 patients with peri-implantitis were recruited and divided into two study groups (11 with healthy periodontium or gingivitis, i.e. no marginal bone loss, and 18 with chronic periodontitis).

Materials and Methods: PISF sample from one implant with peri-implantitis from each patient was collected at the baseline and six months after conservative and surgical peri-implantitis treatment, and clinical parameters were registered. Samples were analysed for MMP-8 with dento ELISA method applying a monoclonal antibody. Mucosal cell samples were also analysed for IL-1 gene polymorphism. PISF MMP-8 levels’ differences between periodontal diagnosis groups and between smokers’ and non-smokers’ were analysed. Also, IL-1 polymorphism profiles were compared between study groups.

Results: PISF MMP-8 levels were higher at the baseline compared to and after the treatment when all sampled implant sites were analysed together (p = 0.001). MMP-8 levels’ distribution was broader in periodontitis patients’ PISF samples, and only in periodontitis patients’ group levels decreased statistically significantly after the treatment (p = 0.005). Smokers’and non-smokers’ PISF MMP-8 was at similar level both at the baseline and after the treatment. No difference between distributions of IL-1 genotypes was found between study groups.

Conclusion: MMP-8 levels increase in peri-implantitis affected implants both in non-periodontitis and periodontitis patients, but levels still after treatment of the condition reflect intensified host response around implants and indicate challenges of controlling peri-Implantitis with any treatment modality.


Host response, Oral implants, Periodontitis, Point-of-care diagnostics

How to cite this article :

René Thierbach, Kurtrt Maier, Timo Sorsa, Päivi Mäntylä. PERI-IMPLANT SULCUS FLUID (PISF) MATRIX METALLOPROTEINASE (MMP) -8 LEVELS IN PERI-IMPLANTITIS. Journal of Clinical and Diagnostic Research [serial online] 2016 May [cited: 2018 Jul 17 ]; 10:ZC34-ZC38. Available from

DOI and Others

DOI: 10.7860/JCDR/2016/16105.7749

Date of Submission: Aug 13, 2015
Date of Peer Review: Oct 07, 2015
Date of Acceptance: Oct 28, 2015
Date of Publishing: May 01, 2016

Financial OR OTHER COMPETING INTERESTS: As declared above.

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