Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2016 | Month : May | Volume : 10 | Issue : 5 | Page : BC09 - BC13

Association of Apo A5 Gene Promoter Region -1131T C Polymorphism (rs662799) to Plasma Triglyceride Level in Patients with Type 2 Diabetic Nephropathy

Abdolkarim Mahrooz, Mehryar Zargari, Vahid Ansari, Atieh Makhlough, Mohammad-Bagher Hashemi-sooteh

1. Faculty of Medicine, Department of Biochemistry, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. 2. Faculty of Medicine, Department of Biochemistry, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. 3. Faculty of Medicine, Department of Biochemistry, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. 4. Faculty, Department of Nephrology, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, IR Iran. 5. Faculty of Medicine, Department of Biochemistry, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

Correspondence Address :
Dr. Mehryar Zargari,
Department of Biochemistry, Cellular and Molecular Research Center,
Mazandaran University of Medical Sciences, Sari, Iran.
E-mail: zargari.mehryar@gmail.com

Abstract

Introduction: Diabetic Nephropathy (DN), a serious complication
of Type 2 Diabetic Mellitus (T2DM), is progressive and
susceptibility to DN varies among T2DM patients. ApoA51131T>C
polymorphism revealed that is strongly associated
with triglyceride levels and proposed as a predisposing factor
for DN.

Aim: The purpose of this study was to investigate the association
-1131T>C ApoA5 gene polymorphism with serum lipids levels
in Type 2 diabetic (DM) patients with or without DN in north of
Iran (Mazandaran province).

Materials and Methods: This study comprised patients with
established T2DM (n=161) and controls (n=58). Genotyping
of APOA5 -1131T>C polymorphisms was performed by PCR–
RFLP. Diabetic patients were divided into two groups: with
nephropathy (DN+, n = 90) and without nephropathy (DN-, n
= 71). Lipids and lipoproteins were assessed by enzymatic
methods.

Results: The genotype frequencies were 63.8 % TT, 31 % TC,
5.2 % CC in controls, 33.8% TT, 52.1 % TC, 14.1 % CC in
DN- and 44.4 % TT, 36.7 % TC, 18.9 % CC in DN+ patients.
The TC genotype and the CC genotype were overexpressed
among DN+ and DN-population in comparison to the control
group. The highest and the lowest TG levels in both diabetic
patients and controls belonged to CC+TC and TT genotypes,
respectively. Furthermore in both patients TG increased with
this order: TT< TC
Conclusion: These results suggest that APOA5 -1131T>C
polymorphisms influence lipid levels in type 2 diabetic
patients.

Keywords

Genotyping, Kidney, Lipids, PCR-RFLP

How to cite this article :

Abdolkarim Mahrooz, Mehryar Zargari, Vahid Ansari, Atieh Makhlough, Mohammad-Bagher Hashemi-sooteh. ASSOCIATION OF APO A5 GENE PROMOTER REGION -1131T C POLYMORPHISM (RS662799) TO PLASMA TRIGLYCERIDE LEVEL IN PATIENTS WITH TYPE 2 DIABETIC NEPHROPATHY. Journal of Clinical and Diagnostic Research [serial online] 2016 May [cited: 2018 Jan 22 ]; 10:BC09-BC13. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2016&month=May&volume=10&issue=5&page=BC09-BC13&id=7895

DOI and Others

DOI: 10.7860/JCDR/2016/19212.7895


Date of Submission: Feb 01, 2016
Date of Peer Review: Mar 09, 2016
Date of Acceptance: Mar 18, 2016
Date of Publishing: May 01, 2016


Financial OR OTHER COMPETING INTERESTS: As declared above.

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