Elevated MDA Level Correlates with Insulin Resistance in Prediabetes BC22-BC24
Dr. Kaushik Kar,
CE 184, Salt Lake City, Kolkata-700064, West Bengal, India.
Introduction: Various metabolic changes in obesity may increase oxidative stress, which if persists continuously may predispose to insulin resistance and diabetes mellitus. Oxidative stress will generate Reactive Oxygen species (ROS) which affects proteins, lipids, carbohydrates and cell membrane. Malon-Di-Aldehyde (MDA) is a lipid peroxidation product found to be elevated in diabetes. Measurement and reduction of oxidative stress in prediabetes may prevent the grave consequences of diabetes.
Aim: The present study was aimed at evaluating MDA levels in prediabetics and diabetics and correlating it with Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) of the same subjects.
Materials and Methods: Plasma MDA and serum insulin were measured in 96 prediabetics and 101 diabetics selected for study and their Body Mass Index (BMI) was calculated. Prediabetic and diabetic patients were divided into two groups, one with BMI>25 kg/m2, and other with BMI<25 kg/m2. Serum MDA was analysed by Thiobarbituric Acid Reactive Substances (TBARS) method. Serum insulin was analysed by Enzyme Linked Immunosorbent Assay (ELISA). HOMA-IR was evaluated from fasting plasma glucose and serum insulin levels.
Results: Insulin resistance was significantly increased in prediabetic (p=0.02) and diabetic (p<0.0001) patients when BMI was >25 kg/m2. Statistically significant positive correlation was observed between MDA and insulin resistance in prediabetic group (p<0.0001).
Conclusion: Findings indicated that raised MDA level correlates with insulin resistance in obese prediabetic patients.