Mutations in Hepatitis-B X-Gene Region: Chronic Hepatitis-B versus Cirrhosis OC31-OC34
Dr. Abdolvahab Moradi,
Department of Microbiology, Faculty of Mcdicine, Golestan University of Medical Science, Gorgan-49177-65181, Golestan, Iran.
Introduction: Specific mutations in Hepatitis-B Virus (HBV) genome would proceed the development of chronic hepatitis B to more serious consequences like cirrhosis and end-stage liver disease.
Aim: This study was designed to detect deletion and insertion mutational patterns in the X-gene region in a population of chronic HBV and related cirrhosis patients.
Materials and Methods: Sixty eight chronic HBV patients and 34 HBV-related cirrhotics were recruited from the eligible cases (N=50) referred to the academic hospitals of Gorgan city, Northeast of Iran, between Jan 2011 to Dec 2013. The HBx region was amplified by semi-nested PCR using serum samples and analyzed by sequencing.
Results: Our findings showed deletions and insertions in the C-terminal of HBx of the cirrhotic group and 8 bp found in two chronic HBV cases (2.9%). We detected 15 types of deletions in cirrhotic cases such as 1762-1768, 1763-1770, 1769-1773 and T1771/A1775.
Conclusion: Considering the proposed role for some of these mutations in severe liver disease, further investigations are needed to clarify the importance of them in the early stages of hepatitis B infection.