Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Table of Contents - Year : 2016 | Month : July | Volume : 10 | Issue : 7 | Page : ZC05 - ZC07

Buccal Micronuclei Assay as a Tool For Biomonitoring DNA Damage in Oral Lichen Planus ZC05-ZC07

S. Vidyalakshmi, R. Madhavan Nirmal, V. Veeravarmal, A. Santhadevy, R. Aravindhan, Sumathy

Dr. R. Aravindhan,
Department of Oral and Maxillofacial Pathology, SRM Kattankulathur Dental College & Hospital,
SRM University, Potheri, Kanchipuram dt, India.

Introduction: The malignant transformation rate of Oral Lichen Planus (OLP) is between 0% and 5.8%. Oral lesions of lichen planus clinically presents itself multifocally, simulating the process of field cancerization in high risk malignancies. The Buccal MicroNucleus Cytome Assay (BMN Assay) provides a platform to identify the high risk individuals by evaluating the markers of nuclear damage at an earliest micro invasive phase.

Aim: To evaluate DNA damage in exfoliated buccal mucosal cells in individuals with oral lichen planus lesions and thereby to delineate the high risk group.

Materials and Methods: Buccal smears from 22 OLP and 10 control samples were stained in modified Feulgen-Rossenback reaction for micronuclei assay. Cytological evaluation of number of MicroNucleated cells (CMN), Total Number of Micronuclei (TMN) in micronucleated cells was done in both groups.

Results: Frequency of micronucleated cells (CMN) when compared among the study and control group, a mean value of 4.27 1.80 and 0.90 0.88 were obtained respectively. On comparing the total number of micronuclei in the micronucleated cells (TMN) between the study and control groups, a mean value of 5.38 2.42 and 1.5 0.88 were obtained respectively.

Conclusion: There was a significant increase in the frequency of micronuclei and the micronucleated cells in the oral lichen planus as compared to normal individuals.