Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Table of Contents - Year : 2016 | Month : May | Volume : 10 | Issue : 5 | Page : BC09 - BC13

Association of Apo A5 Gene Promoter Region -1131T C Polymorphism (rs662799) to Plasma Triglyceride Level in Patients with Type 2 Diabetic Nephropathy BC09-BC13

Abdolkarim Mahrooz, Mehryar Zargari, Vahid Ansari, Atieh Makhlough, Mohammad-Bagher Hashemi-sooteh

Dr. Mehryar Zargari,
Department of Biochemistry, Cellular and Molecular Research Center,
Mazandaran University of Medical Sciences, Sari, Iran.

Introduction: Diabetic Nephropathy (DN), a serious complication
of Type 2 Diabetic Mellitus (T2DM), is progressive and
susceptibility to DN varies among T2DM patients. ApoA51131T>C
polymorphism revealed that is strongly associated
with triglyceride levels and proposed as a predisposing factor
for DN.

Aim: The purpose of this study was to investigate the association
-1131T>C ApoA5 gene polymorphism with serum lipids levels
in Type 2 diabetic (DM) patients with or without DN in north of
Iran (Mazandaran province).

Materials and Methods: This study comprised patients with
established T2DM (n=161) and controls (n=58). Genotyping
of APOA5 -1131T>C polymorphisms was performed by PCR–
RFLP. Diabetic patients were divided into two groups: with
nephropathy (DN+, n = 90) and without nephropathy (DN-, n
= 71). Lipids and lipoproteins were assessed by enzymatic

Results: The genotype frequencies were 63.8 % TT, 31 % TC,
5.2 % CC in controls, 33.8% TT, 52.1 % TC, 14.1 % CC in
DN- and 44.4 % TT, 36.7 % TC, 18.9 % CC in DN+ patients.
The TC genotype and the CC genotype were overexpressed
among DN+ and DN-population in comparison to the control
group. The highest and the lowest TG levels in both diabetic
patients and controls belonged to CC+TC and TT genotypes,
respectively. Furthermore in both patients TG increased with
this order: TT< TC
Conclusion: These results suggest that APOA5 -1131T>C
polymorphisms influence lipid levels in type 2 diabetic