Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

Users Online : 90902

AbstractMaterial and MethodsResultsDiscussionConclusionAcknowledgementReferencesDOI and Others
Article in PDF How to Cite Citation Manager Readers' Comments (0) Audio Visual Article Statistics Link to PUBMED Print this Article Send to a Friend
Advertisers Access Statistics Resources

Dr Mohan Z Mani

"Thank you very much for having published my article in record time.I would like to compliment you and your entire staff for your promptness, courtesy, and willingness to be customer friendly, which is quite unusual.I was given your reference by a colleague in pathology,and was able to directly phone your editorial office for clarifications.I would particularly like to thank the publication managers and the Assistant Editor who were following up my article. I would also like to thank you for adjusting the money I paid initially into payment for my modified article,and refunding the balance.
I wish all success to your journal and look forward to sending you any suitable similar article in future"

Dr Mohan Z Mani,
Professor & Head,
Department of Dermatolgy,
Believers Church Medical College,
Thiruvalla, Kerala
On Sep 2018

Prof. Somashekhar Nimbalkar

"Over the last few years, we have published our research regularly in Journal of Clinical and Diagnostic Research. Having published in more than 20 high impact journals over the last five years including several high impact ones and reviewing articles for even more journals across my fields of interest, we value our published work in JCDR for their high standards in publishing scientific articles. The ease of submission, the rapid reviews in under a month, the high quality of their reviewers and keen attention to the final process of proofs and publication, ensure that there are no mistakes in the final article. We have been asked clarifications on several occasions and have been happy to provide them and it exemplifies the commitment to quality of the team at JCDR."

Prof. Somashekhar Nimbalkar
Head, Department of Pediatrics, Pramukhswami Medical College, Karamsad
Chairman, Research Group, Charutar Arogya Mandal, Karamsad
National Joint Coordinator - Advanced IAP NNF NRP Program
Ex-Member, Governing Body, National Neonatology Forum, New Delhi
Ex-President - National Neonatology Forum Gujarat State Chapter
Department of Pediatrics, Pramukhswami Medical College, Karamsad, Anand, Gujarat.
On Sep 2018

Dr. Kalyani R

"Journal of Clinical and Diagnostic Research is at present a well-known Indian originated scientific journal which started with a humble beginning. I have been associated with this journal since many years. I appreciate the Editor, Dr. Hemant Jain, for his constant effort in bringing up this journal to the present status right from the scratch. The journal is multidisciplinary. It encourages in publishing the scientific articles from postgraduates and also the beginners who start their career. At the same time the journal also caters for the high quality articles from specialty and super-specialty researchers. Hence it provides a platform for the scientist and researchers to publish. The other aspect of it is, the readers get the information regarding the most recent developments in science which can be used for teaching, research, treating patients and to some extent take preventive measures against certain diseases. The journal is contributing immensely to the society at national and international level."

Dr Kalyani R
Professor and Head
Department of Pathology
Sri Devaraj Urs Medical College
Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka
On Sep 2018

Dr. Saumya Navit

"As a peer-reviewed journal, the Journal of Clinical and Diagnostic Research provides an opportunity to researchers, scientists and budding professionals to explore the developments in the field of medicine and dentistry and their varied specialities, thus extending our view on biological diversities of living species in relation to medicine.
‘Knowledge is treasure of a wise man.’ The free access of this journal provides an immense scope of learning for the both the old and the young in field of medicine and dentistry as well. The multidisciplinary nature of the journal makes it a better platform to absorb all that is being researched and developed. The publication process is systematic and professional. Online submission, publication and peer reviewing makes it a user-friendly journal.
As an experienced dentist and an academician, I proudly recommend this journal to the dental fraternity as a good quality open access platform for rapid communication of their cutting-edge research progress and discovery.
I wish JCDR a great success and I hope that journal will soar higher with the passing time."

Dr Saumya Navit
Professor and Head
Department of Pediatric Dentistry
Saraswati Dental College
On Sep 2018

Dr. Arunava Biswas

"My sincere attachment with JCDR as an author as well as reviewer is a learning experience . Their systematic approach in publication of article in various categories is really praiseworthy.
Their prompt and timely response to review's query and the manner in which they have set the reviewing process helps in extracting the best possible scientific writings for publication.
It's a honour and pride to be a part of the JCDR team. My very best wishes to JCDR and hope it will sparkle up above the sky as a high indexed journal in near future."

Dr. Arunava Biswas
MD, DM (Clinical Pharmacology)
Assistant Professor
Department of Pharmacology
Calcutta National Medical College & Hospital , Kolkata

Dr. C.S. Ramesh Babu
" Journal of Clinical and Diagnostic Research (JCDR) is a multi-specialty medical and dental journal publishing high quality research articles in almost all branches of medicine. The quality of printing of figures and tables is excellent and comparable to any International journal. An added advantage is nominal publication charges and monthly issue of the journal and more chances of an article being accepted for publication. Moreover being a multi-specialty journal an article concerning a particular specialty has a wider reach of readers of other related specialties also. As an author and reviewer for several years I find this Journal most suitable and highly recommend this Journal."
Best regards,
C.S. Ramesh Babu,
Associate Professor of Anatomy,
Muzaffarnagar Medical College,
On Aug 2018

Dr. Arundhathi. S
"Journal of Clinical and Diagnostic Research (JCDR) is a reputed peer reviewed journal and is constantly involved in publishing high quality research articles related to medicine. Its been a great pleasure to be associated with this esteemed journal as a reviewer and as an author for a couple of years. The editorial board consists of many dedicated and reputed experts as its members and they are doing an appreciable work in guiding budding researchers. JCDR is doing a commendable job in scientific research by promoting excellent quality research & review articles and case reports & series. The reviewers provide appropriate suggestions that improve the quality of articles. I strongly recommend my fraternity to encourage JCDR by contributing their valuable research work in this widely accepted, user friendly journal. I hope my collaboration with JCDR will continue for a long time".

Dr. Arundhathi. S
MBBS, MD (Pathology),
Sanjay Gandhi institute of trauma and orthopedics,
On Aug 2018

Dr. Mamta Gupta,
"It gives me great pleasure to be associated with JCDR, since last 2-3 years. Since then I have authored, co-authored and reviewed about 25 articles in JCDR. I thank JCDR for giving me an opportunity to improve my own skills as an author and a reviewer.
It 's a multispecialty journal, publishing high quality articles. It gives a platform to the authors to publish their research work which can be available for everyone across the globe to read. The best thing about JCDR is that the full articles of all medical specialties are available as pdf/html for reading free of cost or without institutional subscription, which is not there for other journals. For those who have problem in writing manuscript or do statistical work, JCDR comes for their rescue.
The journal has a monthly publication and the articles are published quite fast. In time compared to other journals. The on-line first publication is also a great advantage and facility to review one's own articles before going to print. The response to any query and permission if required, is quite fast; this is quite commendable. I have a very good experience about seeking quick permission for quoting a photograph (Fig.) from a JCDR article for my chapter authored in an E book. I never thought it would be so easy. No hassles.
Reviewing articles is no less a pain staking process and requires in depth perception, knowledge about the topic for review. It requires time and concentration, yet I enjoy doing it. The JCDR website especially for the reviewers is quite user friendly. My suggestions for improving the journal is, more strict review process, so that only high quality articles are published. I find a a good number of articles in Obst. Gynae, hence, a new journal for this specialty titled JCDR-OG can be started. May be a bimonthly or quarterly publication to begin with. Only selected articles should find a place in it.
An yearly reward for the best article authored can also incentivize the authors. Though the process of finding the best article will be not be very easy. I do not know how reviewing process can be improved. If an article is being reviewed by two reviewers, then opinion of one can be communicated to the other or the final opinion of the editor can be communicated to the reviewer if requested for. This will help one’s reviewing skills.
My best wishes to Dr. Hemant Jain and all the editorial staff of JCDR for their untiring efforts to bring out this journal. I strongly recommend medical fraternity to publish their valuable research work in this esteemed journal, JCDR".

Dr. Mamta Gupta
(Ex HOD Obs &Gynae, Hindu Rao Hospital and associated NDMC Medical College, Delhi)
Aug 2018

Dr. Rajendra Kumar Ghritlaharey

"I wish to thank Dr. Hemant Jain, Editor-in-Chief Journal of Clinical and Diagnostic Research (JCDR), for asking me to write up few words.
Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.

Authors are the souls of any journal, and deserve much respect. To publish a journal manuscripts are needed from authors. Authors have a great responsibility for producing facts of their work in terms of number and results truthfully and an individual honesty is expected from authors in this regards. Both ways its true "No authors-No manuscripts-No journals" and "No journals–No manuscripts–No authors". Reviewing a manuscript is also a very responsible and important task of any peer-reviewed journal and to be taken seriously. It needs knowledge on the subject, sincerity, honesty and determination. Although the process of reviewing a manuscript is a time consuming task butit is expected to give one's best remarks within the time frame of the journal.
Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
Timely publication of journal: Publication of manuscripts and bringing out the issue in time is one of the positive aspects of JCDR and is possible with strong support team in terms of peer reviewers, proof reading, language check, computer operators, etc. This is one of the great reasons for authors to submit their work with JCDR. Another best part of JCDR is "Online first Publications" facilities available for the authors. This facility not only provides the prompt publications of the manuscripts but at the same time also early availability of the manuscripts for the readers.
Indexation and online availability: Indexation transforms the journal in some sense from its local ownership to the worldwide professional community and to the public.JCDR is indexed with Embase & EMbiology, Google Scholar, Index Copernicus, Chemical Abstracts Service, Journal seek Database, Indian Science Abstracts, to name few of them. Manuscriptspublished in JCDR are available on major search engines ie; google, yahoo, msn.
In the era of fast growing newer technologies, and in computer and internet friendly environment the manuscripts preparation, submission, review, revision, etc and all can be done and checked with a click from all corer of the world, at any time. Of course there is always a scope for improvement in every field and none is perfect. To progress, one needs to identify the areas of one's weakness and to strengthen them.
It is well said that "happy beginning is half done" and it fits perfectly with JCDR. It has grown considerably and I feel it has already grown up from its infancy to adolescence, achieving the status of standard online e-journal form Indian continent since its inception in Feb 2007. This had been made possible due to the efforts and the hard work put in it. The way the JCDR is improving with every new volume, with good quality original manuscripts, makes it a quality journal for readers. I must thank and congratulate Dr Hemant Jain, Editor-in-Chief JCDR and his team for their sincere efforts, dedication, and determination for making JCDR a fast growing journal.
Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."

Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
On May 11,2011

Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
Areas for improvement: In certain cases, I felt that the peer review process of the manuscripts was not up to international standards and that it should be strengthened. Also, the number of manuscripts in an issue is high and it may be difficult for readers to go through all of them. The journal can consider tightening of the peer review process and increasing the quality standards for the acceptance of the manuscripts. I faced occasional problems with the online manuscript submission (Pre-publishing) system, which have to be addressed.
Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."

Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
On April 2011

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.

Dr. Anuradha
On Jan 2020

Important Notice

Original article / research
Year : 2011 | Month : June | Volume : 5 | Issue : 3 | Page : 502 - 508

A Study On Surgical Site Infections Caused By Staphylococcus Aureus, With A Special Search For Methicillin-Resistant Isolates


Department of Microbiology

Correspondence Address :
Srinivas R Deshpande
Professor and HOD Biochemistry,
Malabar Medical College, Modakallur, Atholi,
Calicut Dist, Kerala- 670001


Objective: To study the prevalence of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA) in surgical site infections (SSIs) in referral hospitals at the coastal city of Mangalore, India.

Materials and Methods: This study was conducted on 300 patients who underwent surgery in the obstetrics and gynaecology, orthopaedic and surgical departments which were attached to the referral hospitals which were attached to Kasturba Medical College, Mangalore. Pus samples were collected with two sterile swabs and processed in the Microbiology department.

Results: Of the 300 pus samples, 216(72%) showed growth. 258 aerobic bacteria were isolated. The most commonorganism which was isolated was Staphylococcus aureus, with 83(32.2%) isolates. Of these, 8 (9.6%) were Methicillin Resistant Staphylococcus aureus (MRSA).

Conclusions: Indian clinicians and infectious disease specialists in the coastal areas are facing formidable challenges from Methicillin Resistant Staphylococcus aureus. Despite the best surgical practices, nearly a tenth of all the SSIs cases could be caused by MRSA. Routine screening for these multidrug resistant organisms in the hospital staff, especially in the surgical departments and pre-surgical screening of the patients could help in reducing the incidence of MRSA.


Surgical Site Infections, Staphylococcus aureus, Methicillin Resistant Staphylococcus Aureus (MRSA)

Surgical infections which are acquired in the hospital, are recognized to be associated with an extended length of hospital stay, pain, discomfort and sometimes prolonged or permanent disability (1). Surgical site infections (SSIs) are common complications that follow all types of operative procedures (1). These infections are usually caused by the exogenous and endogenous microorganisms that enter the operative wound during the course of the surgery (1). The incidence of the infected surgical wounds may be influenced by factors such as pre-operative care, the theatre environment, post operative care and the type of surgery. The aim of these guidelines (2). has been to reduce surgical site infections by ensuring that the hospital personnel practice optimal preoperative, intraoperative and postoperative care.

The common pathogenic bacteria in SSIs include Staphylococci, Pseudomonas, Streptococci, Enterococci, E.coli, Klebsiella, Enterobacter, Citrobacter, Acinetobacter, Proteus, etc. S.aureus forms a part of the normal flora and can be isolated from the noses of up to 60% of the healthy individuals. It is readily transmitted from person to person onto the hands and clothes of the health care staff, onto objects and into the air (1). (2) S.aureus is the commonest cause of SSI and other nosocomial infections. S.aureus was once susceptible to Penicillin but widely resistant organisms soon emerged. The introduction of Methicillin initially solved the problem, but later, strains which were resistant to Methicillin developed. Thus, an increased number of resistant strains have been seen worldwide (2). (3)

The clinical significance of Methicillin-resistant Staphylococcus aureus (MRSA) is heightened by the fact that these isolates are usually resistant to other anti-staphylococcal agents (Clindamycin, Erythromycin, Tetracycline, sometimes Gentamicin and Trimethoprim/Sulphomethoxazole), with the exception ofVancomycin. Sometimes, Methicillin-resistant-Staphylococcus aureus appears to be susceptible in vitro to other β-lactam agents such as Cephalosporins; however, they are clinically ineffective (3). Since MRSAs are resistant to all the β-lactam antibiotics, the therapeutic options are significantly limited. The incidence of MRSA in India ranges from 30-70% (4). (5). The incidence of nosocomial infections which are caused by MRSA continues to increase; therefore, the importance of their detection, especially for treatment and epidemiological purposes, arises (6). (7) (8). The present study was done in various hospitals in Mangalore to determine the incidence of S. aureus and MRSA in surgical site infections, with the characterization of S.aureus by phage typing.

Material and Methods

STUDY POPULATION: This study was conducted in the Department of Microbiology at four teaching hospitals which were attached to Kasturba Medical College, Mangalore, from October 2003 to September 2004. The study population included three hundred patients suffering from surgical site infections in the surgical and orthopaedic departments of Government Wenlock Hospital, the Obstetrics and Gynaecology department of Lady Goschen Hospital, K.M.C Hospital, Attavar and K.M.C Hospital, Ambedkar Circle, Mangalore. These patients were selected randomly and they belonged to the age group of three to seventy five years. The first two hospitals which have been mentioned are government run hospitals, while the latter two private hospitals belong to the private management of Kasturba Medical College.

A surgical wound with pus discharge, wounds with serous or seropurulent discharge and negative cultures, but with signs of sepsis present concurrently (warmth, erythema, induration and pain) and the physician diagnosis was considered as surgical site infection (7).

Wounds with cellulitis and no drainage and suture abscesses were not included in the study.

A short clinical history regarding the age, sex, type of illness, diagnosis, the type of operation performed, antibiotics given and the presence of associated diseases like diabetes and peripheral vascular disease was obtained.

Pus samples were collected from each patient with the help of two sterile swabs under aseptic precautions, of which one was used for smear preparation and the other was used for culture.

SPECIMEN TRANSPORT: The swabs were brought to the Department of Microbiology, K.M.C. Mangalore, immediately and processed within thirty minutes of collection.

Sample Processing: The pus samples were inoculated onto the media immediately and were incubated at 37°C for 24 hours in 7-10% CO2 concentration. After 24 hours of incubation, the isolated organisms were identified by standard methods (6). (7). Colonies which were suggestive of S.aureus were identified by gram’s staining, catalase test, tube coagulase test and mannitol fermentation tests.

Antibiotic susceptibility testing was done by the disc diffusion test which was described by the modified Kirby Bauer method. The antimicrobial containing discs were placed on the agar plate within 15 minutes of inoculation by using sterile forceps and these were pressed firmly against the plate. The plates were inverted and incubated for 18-24 hours at 35°C, at a CO2 concentration of 7-10%. (9). The drugs were chosen, based on their action on a particular organism and also on the antibiotic policy of the hospitals.

Six discs were used on a 9 cm diameter plate. The antimicrobial discs for Staphylococcus aureus were: Penicillin (10 units), Erythromycin (15mcg), Ciprofloxacin (5mcg), Cefoperazone (30mcg), Oxacillin (l mcg), and Co-trimoxazole (25mcg) .These were tested as first line antibiotics. Those which were resistant to the first line antibiotics were tested with second line drugs like Vancomycin (30mcg), Teicoplanin (30mcg), Rifampicin (30mcg). Cephotaxime (30mcg) and Amoxyclav(30mcg). The antibiotic discs were obtained from HiMedia Laboratories, Pvt. Ltd. Mumbai.

After 18 - 24 hours of incubation, the diameter of the inhibitory zone was measured by using a millimeter scale. The zone size around each antimicrobial disc was interpreted as sensitive, intermediate or resistant, according to The National Committee for Clinical Laboratory Standards (NCCLS), which is now calledthe Clinical and Laboratory Standards Institute (CLSI) criteria. The test of diffusion in the agar was applied according to the CLSI recommendations (CLSI, 2006), by using Mueller-Hinton Agar and antibiotic discs. The NCCLS/CLSI recommends the Cefoxitin disk screen test, the latex agglutination test for PBP2a, or a plate containing 6 μg/ml of Oxacillin in Mueller-Hinton agar which is supplemented with NaCl (4% w/v; 0.68 mol/323wL) as the alternative methods of testing for MRSA (9). (10). (11)Detection of Methicillin - Resistant Staphylococcus aureus, Methicillin—resistant Staphylococcus aureus were detected by the agar screen and the agar dilution method. The strains of Staphylococcus aureus which were resistant to Oxacillin were referred to as Methicillin - Resistant Staphylococcus aureus (MRSA). Oxacillin was used because it is a stable β-lactamase resistant Penicillin.

The agar screen method for the detection of Oxacillin resistance in Staphylococcus aureus: Mueller - Hinton agar (HiMedia Laboratories, Mumbai) which was supplemented with 4% NaCl and 6μg of Oxacillin per ml was used. Inoculum suspensions were prepared by selecting colonies from overnight growth on a blood agar plate. The colonies were transferred to nutrient broth to produce a suspension that matched the 0.5 McFarland’s turbidity standards. This suspension was used to inoculate the Oxacillin agar screen plate by streaking across a small section of the agar surface. The test plates wereincubated for 24 hours at 35°C and were then examined for any evidence of growth which indicated resistance.

Quality Control: Staphylococcus aureus ATCC 25923 (Oxacillin susceptible) and Staphylococcus aureus ATCC 43300 (Oxacillin resistant) were used as the control strains.

The agar dilution method for the detection of Oxacillin resistance in Staphylococcus aureus: Mueller - Hinton agar (pH:7.2) which was supplemented with 2% NaCl was dispensed into screw capped containers in volumes of 19ml and these were sterilized in an autoclave at 121°C for 15 min at 15 lb/in2 pressure. After sterilization, the containers were kept in a water bath at 55°C. One ml of antibiotic solution was added to the 19ml medium, mixed by gentle rotation, poured into a sterile glass petridish and allowed to solidify. Once prepared, the plates were used immediately or stored at 4 - 8°C for not more than 5 days.

The inoculation procedure: Four to five colonies were picked from the overnight growth on blood agar and these were inoculated into 5ml of nutrient broth. The tubes were incubated at 37°C for 2 to 6 hours and the turbidity was matched with 0.5 McFarland’s Std.(Bacterial concentration of 1.5 x 108 colony forming unit/ml). A one in 100 dilutions was done by using normal saline once the inoculum was prepared and these were used within 30 minutes. By using a calibrated loop, 0.01 ml of the inoculum was delivered to the agar surface, resulting in a final inoculum of approximately 104 cfus/ spot. Growth controls were used with each test. Incubation: The plates were incubated at 35°C for 24 hours.

Interpretation of the results:
While reading and interpreting the results, the growth control and the results with the quality control strains were checked (6). (10). The end point was determined for the test by placing the plates on a dark background and by then examining the plates for the lowest concentration of Oxacillin that inhibited the visible growth, which was recorded as the Minimum Inhibitory Concentration (MIC). Staphylococcus aureus strains which had an MIC of Oxacillin as < 2mcg/ml were considered as Methicillin-sensitive Staphylococcus aureus and those having an MIC of >4mcg/ml were considered as Methicillin-Resistant Staphylococcus aureus (MRSA) (10).

Phage Typing:
All the Staphylococcus aureus isolates were sent to Maulana Azad Medical College, New Delhi, for phage typing.Statistical Analysis: P-values were derived from the standard statistical tables. Chi square test was applied to find the statistical significance of various organisms.


Out of the 258 aerobic bacteria which were isolated (Table/Fig 1)133 were gram positive cocci (51.6%) and 125 were gram negative bacilli (48.5%). The most common organism was Staphylococcus aureus, with 83 isolates (32.2%). Other predominant organisms which were isolated were Pseudomonas, Escherichia, Citrobacter, Klebsiella, Proteus, and Enterococcus. Of the 83 Staphylococcus aureus isolates, Penicillin (P) sensitivity was seen in 7 (12.3%), Erythromycin (E) sensitivity was seen in 36 (43.4%), Ciprofloxacin (Cf) sensitivity was seen in 38 (45.8%), Cotrimoxazole (Co) sensitivity was seen in 58 (69.9%), Cefaperazone (Cs) sensitivity was seen in 53 (66.3%), Netillin (Nt) sensitivity was seen in 63 (75.9%) and Oxacillin (Ox) sensitivity was seen in 75 (90.4%) respectively. All the 83 (100%) isolates were sensitive to Vancomycin (Va).

Among the 83 isolates, 8 (9.6%) showed growth, which indicated Methicillin - resistant Staphylococcus aureus and 75 (90.4%) showed no growth, which indicated Methicillin - sensitive Staphylococcus aureus.

The minimum inhibitory concentration of Methicillin for all the 8 isolates(9.6%) which were resistant to Methicillin by the agar screen method, was tested by the agar dilution method. All the 8 isolates showed an MIC of >4mcg/ml. Among the 83 Staphylococcus aureus isolates, 49 were typable and 34 were non-typable. A majority of the typable strains belonged to the phage group III. Among the 8(10%) MRSA isolates, 5 were non-typable. 2 isolates belonged to Group IV respectively. This is depicted in (Table/Fig 2)(Table/Fig 3).


The prevalence rate of surgical site wound infections, though preventable, is high. (13), (14) Several studies all over the world (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) have well established that the early detection of Methicillin resistance is very essential in the prognosis of infections which are caused by S.aureus. Studies by Agarwal (1972), Rao and Harsha (1975), Kowli et al. (1995) and Anvikar (1999) have shown the surgical site infection rates in India to be between 4 to 30%. (16)(17)(18)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)

It has been regularly noted that S. aureus continues to be the single most important bacterial species in the primary aetiology of surgical site infections since the past thirty years or so (15). In our study, we attempted to estimate the prevalence of S.aureus and MRSA in surgical site infections (SSI) in Mangalore, a health conscious, south Indian, coastal city of nearly 1 million population, as there are was a paucity of recent data which was available in this city with four medical colleges and numerous well maintained hospitals. We realize that we have to interpret our findings with caution and are aware that the values may not be representative of the whole of India, but that they may be comparable to those from Kerala, a similarly urbanized state of India. The nutritional status, literacy, nursing facilities and the access to the good health care facilities of Mangalore are comparable to those in the cities of Kerala.

In our study, we could see that, of the 300 pus samples which were collected from the patients with surgical site infections (SSI), the highest incidence of SSIs was seen in orthopaedic cases (52.3%), followed by surgical (30.1%) and obstetrics and gynaecology cases (17.6%) respectively. The orthopaedic case group (n=149) showed statistically significant values (x2=21.02; p value P=.000). Further, we noted that a majority of the orthopaedics patients (98) who were included in the study were operated for the open reduction of fractures. Contamination from the external environment may be a possible reason for the higher rate of surgical site infections (SSI) in orthopaedic surgeries. Among them, the number of male patients (63%) was higher than the female patients (37%). This could be due to increased mobility in the male population. In both the sexes, a maximum number of patients belonged to the age group of 21-30 years; males (25.9%) and females (22.2%) respectively.

The P value (P=0.000) was highly significant with respect to government hospital surgeries when compared to private hospital surgeries. This high statistical significance of surgical site infections in the government hospitals of Mangalore which were attached to the same medical college, as compared to the two private hospitals of Kasturba Medical College, Mangalore, may be due to the relatively poor hygienic measures in the government hospitals, and this needs further confirmatory studies. The common denominator in our study and in Udgaonkar’s study (16)(17)(18)(19)(20) was that a majority of the samples were derived from government hospital patients. The changing pattern could be due to the reported indiscriminate use of new/many broad spectrum antibiotics, the increasing use of instrumentation, the long preoperative stay and coexisting infections at a remote body site (20)-(21). Also, another limitation of our study was the use of the oxacillin disc. The cefoxitin disc diffusion method and the polymerase chain reaction method for the detection of the mecA gene, which have been currently recommended by the CLSI criteria, would have been more sensitive in the detection of the MRSA isolates. (13), (14).

Again, out of the 300 pus samples, 216 (72%) showed growth. Our results are similar to a study on SSIs by Soleto et al (2003, Bolivia) (21)-(22), who reported 75.6% growth. A totalof 258 aerobic bacteria were isolated from the 300 samples. Staphylococcus aureus was the most common pathogen and it grew in 83 samples (32.2%), which was found to be statistically highly significant (p value: 0.000).This was comparable to the study which was conducted by Edwards et al (15), who reported a high incidence of S. aureus (30.3%). Also, previous studies in India by Surange et al (17) and Subramanian et al (12) have reported the incidence of S. aureus to be 34.2% and 30.9% respectively. Again, recent studies on SSIs in India by Kownhar et al in 2008, have shown an incidence of 37% [23-31] and those done in USA by Eagye et al (32) have shown an incidence of 39% of S.aureus. A higher predominance of S.aureus (51.6%) was observed by Keith et al (29) in older operative patients. It is our postulation that there may be more S.aureus carriers, as the hospital staff could be silent carriers and this finding may be relevant to the city hospitals of Mangalore also. Four isolates of S. aureus were seen with Pseudomonas species and Escherichia coli respectively. Two isolates each were seen along with Proteus species, Acinetobacter species and Group A beta haemolytic Streptococcus.

The distribution of the bacteria which were isolated from our study on SSIs and from various previous studies as compared to our study is given in (Table/Fig 4) . While studying the antibiotic sensitivity patterns, we observed that Vancomycin, Rifampicin, Linezolid and Teicoplanin were the most sensitive (100%) antibiotics for S.aureus. Among the first line antibiotics, the highest sensitivity was recorded with Oxacillin (90.4%) and the least sensitivity (12.3%)[ was seen with Penicillin. Other effective antibiotics were Erythromycin( 43.4%) ,Ciprofloxacin ( 45.8%) ,Cefoperazone ( 66.3%) and Netillin ( 75.9%).

S.aureus has been known to acquire resistance to most antibiotics including the penicillinase resistant ones like Methicillin. A study by Weigelt et al in USA, found an incidence of 20.6% MRSA (33) in SSIs. Still higher incidences of 45% and 58.2% MRSA have been documented by Eagye et al (32) and Keith et al (29). . The incidence of Methicillin–resistant S.aureus in our study was 8 (9.6%).This finding is in agreement with the reported incidence of (10%) MRSA in a study which was conducted by Agarwal and Khanna (35). But, observations by Kownhar et al(23) have showeda higher incidence of 21.7% . We found that all the MRSA strains (100%) were sensitive to Vancomycin, Rifampicin, Teicoplanin and Linezolid .This finding could have relevant clinical use in the antibiotic policy guidelines for hospitals.

Of the 83 isolates of Staphylococcus aureus which were sent to the Maulana Azad Medical College, New Delhi, for phage typing, only 49 were typable ( 59%) while the rest of the 41% were non typable .These phage typing values are similar to those which were found in the study by Agarwal et al (16), who reported 58.5 percent typable and 41.5% untypable strains. The predominant phage group in our study was group 3 (57%), as in Agarwals’s study. Among the 8 (10%) MRSA isolates, five were non typable, while two isolates belonged to group III and one isolate to group IV respectively.

It is an undisputed fact that the infection may originate from the patient’s normal flora or that it may be derived from the hospital environment. Multidrug resistant S.aureus is commonly associated with the hospital environment. In the present study, 32.2% of the postoperative wound infections were due to this organism and this indicates that the possible source of infection in these cases may be from the hospital environment. This fact needs to be kept in mind by the surgeons. Also, is well known that surgical site infections are also associated with significant increases in the length of hospital stay, additional costs, morbidity and mortality. (23)(24)(25) The widespread availability and the use of antimicrobial agents for prophylaxis seem to have altered the surgical practice in the past twenty years.

Since MRSA strains are highly resistant to many groups of antibiotics, there is an increased rate of morbidity and mortality in the ICU facilities, as well as in immuno-compromised individuals. (18) Staphylococcal resistance to oxacillin/methicillin occurs when an isolate carries an altered Penicillin-binding protein, PBP2a, which is encoded for by the mecA gene. (22),[28 ] This penicillin-binding protein binds beta-lactams with lower avidity, which results in resistance to this class of antimicrobial agents. (21), (22), (23) Recently, Harbarth et al. (2008) have observed that methicillin-resistant S. aureus (MRSA) alone constituted 5.1% of the surgical site infections (24).

Further, it has been established by Classen et al (1992) that prophylactic antimicrobials are often not administered at the optimal times, to ensure their best presence in effective concentrations peri-operatively (25).This point influences most surgical outcomes (27), (28), (29). It is highly desirablethat prophylactic antibioticsshould be administered within one hour before the operation and that they should be continued for only 24-72 hours post operatively, as a policy to avoid multidrug resistance. Inappropriate dosage and the duration of antibiotic use not only fail to reduce the infection, but also lead to an increase in multidrug resistant mutants due to selection pressures. (28), (29) Hence, the prevention of SSIs is essential and this poses a major challenge in any healthcare system. (28) Infection control measures such as the active surveillance of SSIs, the implementation of a checklist, compliance observations and instruction/training of healthcare workers, as well as Staphylococcus aureus /MRSA screening, clipping instead of shaving, adherence to perioperative antibiotic prophylaxis, maintaining intraoperative normothermia and blood glucose control are essential in order to prevent SSIs. (29)(30)(31) The prevention of nosocomial infections is not only highly desirable for patients, but it is now seen as a major political priority in all the hospitals, irrespective of whether they are private or public .Guidelines and protocols for basic infection control practices such as hand washing and written protocols for the safe insertion and maintenance of devices such as intravascular catheters, should be widely available and adhered to. (31), (32), (33), (34)

Further, we feel that the high incidence of MRSA could be reduced by screening for these organisms routinely in all the hospital staff and that the nation’s celebrated health care facilities should lead to the change here. Relevant policy and protocol changes could usher in a culture of cleanliness and abundant caution in the operation theatres. The Municipal Corporation of the Indian cities can pitch in implementing and monitoring operation theatre protocols (31). A pre-surgical screening of the patients and treating those who carry these bugs would definitely help in reducing infections, the duration of hospital stay and the economic burdens which are associated with these. (33), (36)It is high time for the Indian hospital managements to ensure a regular, close clinical liaison between the surgical team, the microbiologist, and the infection control team to provide proper quality surgical services. (33)(34)(35)(36)(37)(38) Regular periodic studies could be established by the Indian medical colleges to control and conquer the resistant organisms in surgical site infections. The national governments should formulate national standard operating procedures and infection control standards to face the challenges which are posed by antibiotic resistant organisms (36),(37).

As Dr. Vincent Cheng explains, the anti-MRSA success of Hong Kong (37) is a consequence of several factors – an aggressive approach, single room isolation, and contact screening. For various reasons, almost every cluster of cases was reported in the press. Nonetheless, in the UK at least, stories of “dirty hospitals” are evocative, with public acceptance, leading to a cycle of reinforcement that eventually transforms the history of MRSA control in the country. (37) Since 2003, Hong Kong has leaped a big step forward and there is no reason why we can’t emulate Hong Kong. Indian governments should act more proactive and emulate anti-community acquired MRSA measures as aggressively as United Kingdom and Hong Kong and make it notifiable.The judicious use of antibiotic prophylaxis and the use of an organized system of wound surveillance and reporting can help in reducing the wound infection rate to an attainable minimum. (37) It is clear that the two papers in Lancet (August 2010 and April2011) have at last shaken the Indian government (38), which has initially been in denial, out of its slumber and inaction. The Indian Council of Medical Research has invited research proposals from scientists to generate scientific evidence on antimicrobial resistance. This move indicates that the apex medical research body has finally realised there is no place for jingoism in matters of science, and that the latest findings on antibiotic resistance must be taken seriously and verified scientifically. (38) This exercise will prove to be useful only if the researchers are truly free to report the presence of the superbugs and the extent of their spread. The second important development has been the drafting of the much-needed national policy for the containment of antimicrobial resistance. The policy admits that the use of antibiotics is inappropriate in 20 per cent to 50 per cent of the cases. It targets the indiscriminate use of antibiotics in food animals and intends to curb the practice, since it ultimately causes drug resistance in humans. Most importantly, the access to third generation antibiotics like carbapenems is to be restricted to Indian tertiary hospitals. (38) But even in these hospitals, efforts must be directed towards restricting their use to patients with severe infections. The Indian government should waste no time in creating a national surveillance system for measuring antibiotic resistance if it is serious about getting on top of the problem. (38) By all parameters, our war against antibiotic-resistant bacteria is far from winning. The question is - are we serious? Will the Indian government do its role and save the hospitals and its public?


Our most common surgical site infection isolate was Staphylococcus aureus (32.2%). Methicillin resistant Staphylococcus aureus (MRSA) constituted 9.6% of the total SSI isolates. Due to the increased morbidity and mortality which are associated with these drug resistant organisms, an early detection and intervention is a prerequisite in surgical patients. Although surgical wound infections cannot be completely eliminated, a reduction in the infection rate to a minimal level could have significant benefits, by reducing postoperative morbidity and mortality, and the wastage of health care resources. Antimicrobial therapy should be designed to deliver an adequate drug concentration to the site of infection. The governments should take proactive steps in setting up national hospital antibiotic policy guidelines and in instituting a culture of simple anti-MRSA measures like hand washing among the health care personnel. Hospitals should screen for MRSA among their staff and treat the hospital staff who are affected by the bacteria.


Dr. Gayathree would like to thank her teachers, Dr. Shalini Shenoy and Dr.Srikara Mallya for their guidance during the study.


Nichols RL. Current Strategies for Prevention of Surgical Site Infections: Curr Infect Dis Rep.2004 Dec; 6(6):426-434.
Mangram AJ, Horan TC, Pearson ML, Silver LC, Jarvis WR.Guidelines for Prevention of SSI, 1999,Criteria for defining SSIs. The Hospital Infection Control Practices Advisory Committee. Infection control and Hospital Epidemiology 1999; 20; 4; 24. Available at hip/SSI/SSI.pdf.
Susan LK. Concepts in Antimicrobial therapy; TB of Diagnostic Microbiology: 3rd edition:; WB Saunders. Editors; Mahon C.R, Manuselis G:2007 pg 82.
Verma S, Joshi S, Chitnis V, Hemavani N, Chitnis D. Growing problems of Methicillin Resistant Staphylococci- Indian Scenario. Indian J Med Sci 2000; 54:535-40.
Bratzler DW, Hunt DR. The surgical infection prevention and surgicalcare improvement projects: national initiatives to improve outcomes for patients having surgery. Clin Infect Dis 2006; 43:322-330.
Bannerman TL. Staphylococci and other catalase positive cocci that grow aerobically. In: Manual of Clinical Microbiology, 8th Ed. Washington DC: ASM Press In: Murray PR, Baron EJ, Jorgensen JH, editors;2003; p.384-404.
Oslon M, Connor O, Schwartz ML .A Five year Prospective Study of 20193 Wounds at Minneapolis V a Medical Center. Ann Surg.1984; 199; 253-9.
Larsen HS, Connie RM. Staphylococci: TB of Diagnostic Microbiology 3rd edition; WB Saunders. Editors; Mahon CR, Manuselis G:2007; pg 341.
Howard BJ. Nosocomial Infections- an Overview. Text Book of Clinical and Pathogenic Microbiology, 1994:2nd Ed. Howard BJ (Editor Mosby- Year Book Inc., St. Louis, MO, USA.
Mandell, Douglas Bennett’s Principles and Practice of Infectious Diseases 4th ed. Churchill Livingstone. Editors: Mandell GL, Bennet JE and Dolin R: Surgical and Trauma related Infections: Vol 2, 1995 Chapter 296 pp.2742-54.
Dixon G: Sources of Surgical Infection; Surgery: 2002; pp 179-196.
Subramanian K.A, Prakash A, Shrinvas, Bhujwal RA. Post-operative wound infection. Ind J Surg 1973; 57-64
Gaynes RP, Culver DH, Horan TC, Edwards JR, Richards C, Tolson JS. Surgical site infection (SSI) rates in the United States, 1992-1998: the National Nosocomial Infections Surveillance System basic SSI risk index. Clinical Infectious Diseases 2001; 33: Suppl 2:S69-77
Suchitra Joyce B. and Lakshmidevi N. Surgical site infections: Assessing risk factors, outcomes and antimicrobial sensitivity patterns African Journal of Microbiology Research Vol. 3 (4) pp. 175-179 April, 2009. Available online at
Edwards LD: The Epidemiology of 2056 Remote Site Infections and 1966 Surgical Wound Infections occurring in 1865 patients: Annals of Surg: 1976; 184; 758-66.
Agarwal SL: Study of postoperative wound infections: Ind.J.Surg.1972:314-20.
Surange BN, Rai UK: Bacteriological Pattern and their sensitivity to antibiotics in Wound Infections. Ind. J. Path. Micro: 1979; 22; 331-36.
Anvikar AR, Deshmukh AB, Karyakarte RP, Damle AS, Patwardhan NS, Malik AK, Bichile LK, Bajaj JK, Baradkar VP, Kulkarni JD, Sachdeo SM . A one year prospective study of 3,280 surgical wounds. Indian J. Med. Microbiol. 1999;17: 129-32.
Oslon MM, Jane RN and Lee T: Infection Surveillance; Arch Surg 1990; 125; 794-803.
Udgaonkar, Bhavthanker A: Bacteriological study of Postoperative wound infections. JIMA; 1985; 83; 231-35.
Soleto L et al: Incidence of surgical- site infections and the validity of the National Nosocomial Infections Surveillance system risk index in a general surgical ward in Santa Cruz, Bolivia: Infect Control Hosp. Epidemiology:2003:24(1)26-30.
Kowli SS, Nayak MH, Mehta AP, Bhalerao RA Hospital infection.Indian J. Surg. 1985;48: 475-86.
Kownhar H, Shanker EM, Vignesh R, Sekar R, Velu V, Rao VA: High rate of isolation of S.aureus from Surgical Site Infections in an Indian Hospital; J Antimicrobial Chemotherapy-Vol 61,Suppl 3 pp 758-760:Oct 2008.
Harbarth S, Fankhauser C, Schrenzel J, Christenson J, Gervaz P,Bandiera-Clerc C, Renzi G, Vernaz N, Sax H, Pittet D ;.Universal Screening for Methicillin-Resistant Staphylococcus aureus at Hospital Admission and Nosocomial Infection in Surgical Patients.JAMA.2008; 299: 1149-1157.
Classen DC, Evans RS, Pestotnik SL, et al. The Timing of Prophylactic administration of antibiotics and risk of Surgical wound Infections N Engl J Med. 1992; 326:281–286
Wayne. Clinical and Laboratory Standards Institute / NCCLS Performance standards for Antimicrobial disc diffusion tests; Approved standards. 9th ed. CLSI Document M2-M9. Clinical and Laboratory Standards Institute; 2006;4456.
Miller LG, Diep BA. Clinical practice: colonization, fomites, and virulence: rethinking the pathogenesis of community associated methicillin-resistant Staphylococcus aureus infection. Clin Infect Dis 2008; 46:752-60.
Lilani SP, Jangale N, Chowdhary A, Daver GB. Surgical site infection in clean and clean-contaminated cases. Indian Journal of Medical Microbiology 2005; 23: 249-52
Keith SK, Deverick JA, Sloane R, Chen LF, Choi Y, Link K, Sexton DJ, Schmader KE: The impact of Surgical Site Infection on older operative patients: J Am Geriatr Soc.2009 January;57(1):46-54.
Rao AS, Harsha M (1975). Postoperative wound infections. J Indian Med. Assoc. 64:90-3.
Chaudhary U, Aggarwal R (2004). Extended spectrum beta -lactamases (ESBL)-an emerging threat to clinical therapeutics.Ind. J. Med. Microbiol., 22(2): 75-80.
Eagye KJ, Kim A, Laohavaleeson S, Kuti JL, Nicolau DP: Surgical Site Infection: does inadequate antibiotic therapy affect patient outcomes?: Surgical Infect(Larchmt).2009 Aug;10(4):323-31.
Weigelt JA, Lipsky BA, Tabak YP, Derby KG, Kim M, Gupta V: Sugical Site Infections: Causative pathogens and associated outcomes; Am J Infect Control,2010 Mar;38(20:112-20.Epub
Anand KB, Agrawal P, Kumar S, Kapila K: Comparison of Cefoxitin Disc Diffusion Test, Oxacillin Screen Agar, and PCR For mecA Gene For Detection Of MRSA: Indian Journal of Medical Microbiology, (2009) 27(1): 27-935] Aggarwal A, Khanna S, Arora U, Devi P.Correlation of β-Lactamase Production/ Methicillin Resistance and Phage pattern of Staphylococcus aureus: Ind. J Med. Sciences: 2001:55:253-256.
Rajaduraipandi K, Mani KR, Paneerselvam K, Mani M, Bhaskar M and Manikandan P. Prevalence and antimicrobial susceptibility pattern of Methicillin Resistant Staphylococcus aureus: a multicentre study. Indian J Med Microbiol 2006; 24:34-8.
Pak-leung Ho .A Losing Battle against Extensively-resistant Bacteria? The Hong Kong medical Diary. In: Editorial.Vol.16 No.4 April 2011.
The superbug effect. Opinion: Editorial: The Hindu. India’s national newspaper dated April 22, 2011. editorial/article1718590.ece

DOI and Others


JCDR is now Monthly and more widely Indexed .
  • Emerging Sources Citation Index (Web of Science, thomsonreuters)
  • Index Copernicus ICV 2017: 134.54
  • Academic Search Complete Database
  • Directory of Open Access Journals (DOAJ)
  • Embase
  • EBSCOhost
  • Google Scholar
  • HINARI Access to Research in Health Programme
  • Indian Science Abstracts (ISA)
  • Journal seek Database
  • Google
  • Popline (reproductive health literature)