Staining Effect of Amflor, Zerosense and Ceti-F Mouthwashes on Dental Enamel: In-vitro Study
Correspondence Address :
Dr. Sowmithra Devi Saravanan,
No. 162, Saveetha Dental College, Poonamalle High Road, Chennai, Tamil Nadu, India.
E-mail: sowmithrasaravanan@gmail.com
Introduction: Chemical methods of plaque control, especially mouthwashes, offer great help in maintaining good oral hygiene and preventing dental caries. However, these mouthwashes often result in staining of the enamel.
Aim: To evaluate discolouration caused by three different fluoride mouthwashes on dental enamel.
Materials and Methods: This was an in-vitro study that included 40 freshly extracted premolar teeth and was conducted at University of Saveetha in January 2021. A total of four groups were made with 10 teeth in each group. Group 1 included teeth as the control group where distilled water was used, Group 2: Amflor mouthwash group, Group 3: Ceti-F (Cetyl Pyridinium Chloride, Triclosan and Sodium Fluoride) mouthwash group and Group 4: Zerosense mouthwash group. Pretreatment colour change examination according to CIELAB {International Commission on Illumination L: value or degree of lightness, a: positions on red/green (+a=red, -a=green); b: yellow/blue (+b=yellow, -b=blue) axes} colour space system was done before immersing in the mouthwash solution (T1). Then each group of teeth were immersed in their respective mouthwash for 24 hours and postimmersion colour change was determined (T2). Analysis of Variance (ANOVA) test was done to determine intergroup comparison of all four groups in terms of differences in colour changes between T1 and T2. Intragroup comparison using paired t-test was done to find out colour differences within the study groups.
Results: On observing total sample of 40 freshly extracted premolar teeth (10 teeth in each group. Group 1-control group, Group 2-Amflor mouthwash group, Group 3-Ceti-F mouthwash group and Group 4-Zerosense mouthwash group. ANOVA test revealed a statistically significant difference between T1 and T2 for the three mouth washes (p<0.05). Paired t-test for intragroup comparison revealed a statistically significant colour difference in all the three mouthwash groups except for the control group (p<0.05). The colour change (?E) value was significantly higher for the Amflor group, and least for the Zerosense group. An obvious colour change was noted for all the mouthwash under study.
Conclusion: Fluoride mouthwashes produce an obvious staining effect on dental enamel. Among the three test groups included, Zerosense showed the least colour change.
Caries prevention, Chemical plaque control, Discolouration, Fluoride mouthwashes, Remineralising agents, White spot lesion
10.7860/JCDR/2021/50440.15661
Date of Submission: May 19, 2021
Date of Peer Review: Aug 07, 2021
Date of Acceptance: Oct 06, 2021
Date of Publishing: Nov 01, 2021
Author declaration:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? NA
• For any images presented appropriate consent has been obtained from the subjects. NA
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