Association of Arg72Pro Single Nucleotide Polymorphism of p53 Gene with Functional Outcome after Traumatic Brain Injury: A Meta-Analysis
Dr. Usha Sachidananda Adiga,
Department of Biochemistry, KS Hegde Medical Academy, NITTE (Deemed to be University), Mangalore-575018, Karnataka, India.
Introduction: Traumatic Brain Injury (TBI) is one of the major causes of death and disability. The p53 gene, guardian of genomes has a key role in programmed cell death and repair mechanisms of damaged DNA. Single Nucleotide Polymorphism (SNP) of this gene may have a role in functional outcome after TBI, There is only limited research carried out on the SNP of Arg72Pro of p53 and there is no meta-analysis or systematic reviews carried out on this area to the best of our knowledge.
Aim: The aim of the study was to find the association of Arg72Pro Polymorphism of p53 with the functional outcome after TBI by conducting a meta-analysis.
Materials and Methods: Literature search was systematically carried out by browsing, PubMed, Google Scholar, and Scopus for original research articles with the keyword,â€™p53 Gene Polymorphism and TBIâ€™. Out of 10 articles retrieved, only three were eligible as per the inclusion criteria. Studies which assessed Arg72Pro Polymorphism of p53 and functional outcome by Glasgow outcome scale at discharge after TBI were included.
Results: Statistical analysis was carried out using Medcalc, Random effect models were used to calculate OR and CI. The significance of summary ORs was determined with a Z test. Heterogeneity assumption was checked by x2-based Q test. Forest plot was used to study the heterogeneity of the studies, the funnel plot was used to assess the publication bias. Results showed that Arg/Arg SNP of the p53 gene is associated with poor outcome, with a pooled OR of 2.636, with a 95% confidence interval of 1.376-5.048, p=0.003. No publication bias was observed.
Conclusion: Single nucleotide polymorphism of p53, Arg72Arg is associated with poor outcome after TBI when assessed at discharge. However, there is a need for further primary research to confirm this association and also the study reflects that further gene loci which may influence the outcome can be explored.