The Synergistic Hypocholesterolaemic and Immunomodulatory effect of two probiotic strains in vivo 312-315
Dr. Aruna Bhatia, Professor Immunology and Immunotechnology
laboratory, Dept of Biotechnology Punjabi University, Patiala-
147002, Punjab, India.
E-mail: email@example.com, Phone: +91-9878263077
Probiotics are known to show beneficial effects to the host. But the effect varies with the species and the strain of the probiotics. The present study was designed to study the functional relationship of two probiotic strains. Lactobacillus delbruckii 405 and Lactobacillus casei subsp. casei 17 were tested in vivo separately and in a 1:1 combination (109cells /ml) for their hypocholesterolaemic and immunomodulatory potential in swiss albino mice. Two sets of experimental groups were made. In the experimental group 1, the effects of Lactobacillus spp. alone or in combination was assessed for hypocholesterolaemic activity and in the experimental group 2, the effects of the probiotic alone or in combination were observed on immune response.The Development of Antibody Titers, the Delayed Type Hypersensitivity test, the Nitroblue Tetrazolium Reduction test, the Inducible Nitric Oxide Synthase test and the Bactericidal Activity test were the tests which were employed to assess the functions of different immunocytes. The data of the tests were statistically analyzed by using oneway ANOVA, followed by Turkey’s Multiple Range Test which was applied for post hoc analysis. Results and Conclusion: It was concluded that the two Lactobacilli strains acted synergistically in vivo, as the effect was more profound in the lowering of cholesterol levels and in the augmentation of the immune system.