A Potential Correlation between Systemic Oxidative Stress and Intracellular Ambiance of the Lens Epithelia in Patients with Cataract
Madhur M. Goyal,Assistant Professor
Department of Biochemistry,J.N.Medical]
College,Datta Meghe Institute of
University, Wardha-442001 (MH),(INDIA),
Aim: To study the correlation between systemic oxidative stress and intracellular ambiance of the lens epithelia in patients with cataract.
Materials and Methods: Spectrophotometery was employed for the estimation of catalase activity and the extent of lipid peroxidation in the lens epithelial cells (LEC) and plasma. Both are markers for oxidative stress. No antioxidant medicines were used by the cataract patients enrolled in this study; otherwise, they were all healthy individuals without any systemic diseases.
Results: A total of 56 patients with cataract were included in this study. The mean ages of the patients were 66.6 Â± 8.3 (Â±SD) years for males and 62.4 Â± 10.0 years for females. Catalase activity was estimated in surgically removed LEC (221.16Â±135.87 U/Âµg protein; MeanÂ±SD) and in plasma (277.56Â±162.44 KU/mg Hb). MDA levels were also calculated in LEC (1.28Â±0.79nM/mg protein) and in plasma (537.30Â±238.47 nM/g protein). Linear regression analysis showed a partial positive correlation in LEC and plasma catalase activity (r: 0.701; p<0.05), but not in MDA (r: 0.248; p>0.05).
Conclusion: Increased systemic oxidative stress can lead to the development or progression of cataract by affecting the intracellular ambiance of the lens epithelia. So, subjects having high systemic oxidative stress are more vulnerable for the development of cataract.