Detection Of Biofilm Producing Staphylococci: Need Of The Hour
Dr. S. Bose,Professor of Microbiology J. N. Medical College,Sawangi (M), Wardha-442004 (M.S.)E-mail: firstname.lastname@example.org,email@example.com
Biofilms are group of microorganisms encased in an exopolymeric coat. They have been associated with a variety of persistent infections that respond poorly to conventional antibiotics. In this study detection of biofilm productions by Staphylococcus spp. was done by using Congo red agar (CRA) methods, tube methods (TM) and tissue culture plate (TCP) methods. Out of 179 Staphylococcus spp., 111 were S.epidermidis and 68 were S.aureus. 44.69% of S.epidermidis and 32.96% S.aureus were slime producers. 97 isolates were detected as slime producer by TCP method, 76 by TN and 11 by CRA method. High resistances to conventional antibiotics were shown by biofilm producers.
This study summarized the prevalence, antibiotic sensitivity pattern and suitable and reproducible method for detection of biofilm producing Staphylococci.