Serum Paraoxonase Activity In Normolipidaemic Patients With Acute Myocardial Infarction
Dr. Arun Kumar Department of Biochemistry Manipal College of Medical Sciences Deep Heights, Pokhara, Nepal email:email@example.com
Background: Although studies have demonstrated that HDL-C associated paraoxonase is involved in protection of LDL-cholesterol and other lipoproteins from the deleterious effects of oxygen free radicals in ischaemia and reperfusion, there are controversial data on the correlation between paraoxonase activity and the ischaemia process.
Aim: The present study was planned to evaluate the paraoxonase activity in patients with acute myocardial infarction (AMI), with normal lipid profile.
Setting and Design:Serum paraoxonase activities were determined in 165 normolipidaemic patients diagnosed with AMI, and 165 age and sex matched healthy volunteers served as controls.
Material and Methods: Serum Paraoxonase activities were measured by using an enzymatic kit manufactured by the Zeptomatrix Corporation, New York, USA, for AMI patients and controls. Also, the lipid profile was analyzed enzymatically in these subjects.
Statistics: The values were expressed as means Â± standard deviation (SD), and data from patients and controls was compared by using studentâ€™s â€˜tâ€™-test.
Results and Conclusion: Serum paraoxonase activity was significantly decreased in AMI patients as compared to controls (p<0.001). Also, total cholesterol levels, TC/ HDL-C ratio, triglyceride levels, LDL-cholesterol levels, LDL-C/ HDL-C ratio and TG/HDL-C ratio were significantly higher (p<0.001), and HDL-cholesterol levels were significantly lower in AMI patients (p<0.001). No correlation was observed between PON1 activity and HDL-C levels in patients and controls. These findings suggest that decreased paraoxonase activity could be due to increased oxidative stress in AMI.