qRT-PCR Compliments Immunohistochemistry In Archival Breast Cancer Samples
Dr.Prabhu J S Triesta Sciences, India Pvt. Ltd Institute Of Population Health And Clinical Research Building St Johnâ€™s Medical College CampusOpp. Bda Complex,Koramngala,Bangalore-560034
Background: Molecular characterization of tumour tissue in limited archived formalin fixed, paraffin embedded (FFPE) sample is always challenging. Better molecular characterization will not only help in accurate diagnosis but also in prognosis and guides therapeutic decisions.
Study Design: In the present study we tested the prognostic markers of breast cancer, estrogen receptor (ER) and progesterone receptor (PgR) by immunohistochemistry and quantitative real time polymerase chain reaction (qRT-PCR) on archived FFPE samples.
Results: High concordance was observed between the two methods for ER and PgR.
Conclusion: We conclude qRT-PCR may be used as an alternative method for the study of prognostic factors in archived tissues. Moreover, qRT-PCR can be a high throughput method for evaluation of markers with limited archived tissue.