Year :
2015
| Month :
May
| Volume :
9
| Issue :
5
| Page :
DC11 - DC14
Full Version
Prevalence of Enterotoxin Genes and
spa Genotypes of Methicillin-resistant
Staphylococcus Aureus from a Tertiary
Care Hospital in China
Published: May 1, 2015 | DOI: https://doi.org/10.7860/JCDR/2015/.5990
Yanmeng Li, Ruike Zhao, Xianfeng Zhang, Qingzhen Han,
Xuefeng Qian,Guohao Gu, Jinfang Shi, Jie Xu
1. Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.
2. Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.
3. Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.
4. Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.
5. Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.
6. Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.
7. Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou,P.R. of China.
8. Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou,P.R. of China.
Correspondence Address :
Dr. Jie Xu,
Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University,
No. 188, Shizijie, Canglang District, Suzhou, 215006, P.R. China.
E-mail : xuj2007@lzu.edu.cn
Abstract
Objectives: Methicillin-resistant Staphylococcus aureus (MRSA)is a major nosocomial pathogen that causes a variety of infections. MRSA has evolved resistance to multiple antibiotics. Genetic background and virulence differs in different geographic regions. The present study was aimed to investigate the prevalence of enterotoxin genes and spa genotypes of hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) isolated from a tertiary care hospital of Jiangsu province, China.
Materials and Methods: HA-MRSA isolates from August 2013 to April 2014 at a tertiary care hospital of China were collected. We investigated antimicrobial pattern, spa types, SCCmec types and the presence of 14 virulence genes.
Results: Eighty HA-MRSA isolates were collected. Results from SCCmec typing revealed that 73.8% were type II; 13.8% were type III; 12.5% were type V. There were 19 different spa types. Spa type t2460 was the most common (35.0%), followed by t002 (11.3%). CC5 was the predominant MLST CCs type (50%). The most frequent toxin genes were sea, seb, sed, sel, sen and seo (100.0%). None of the investigated isolates carried the sec or tst.
Conclusion: Genotypic and virulence evaluation of the isolated HA-MRSA revealed that the isolates with CC5 and SCCmec II were the predominant type and highly homological. The virulence profiles mainly existed in the genes of sea, seb, sed, sel, sen, seo and ser. The prevalence of t2460 was an outbreak and the predominant spa type.
Keywords
sea, seb, sed, sel, sen, seo, ser, t2460
DOI: 10.7860/JCDR/2015/12090.5990
Financial OR OTHER COMPETING INTERESTS: None.
Date of Submission: Nov 13, 2014
Date of Peer Review: Feb 17, 2015
Date of Acceptance: Apr 02, 2015
Date of Publishing: May 01, 2015
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