Year :
2015
| Month :
January
| Volume :
9
| Issue :
1
| Page :
EC05 - EC07
Full Version
Angiogenesis in Breast Cancer and its Correlation with Estrogen, Progesterone Receptors and other Prognostic Factors
Published: January 1, 2015 | DOI: https://doi.org/10.7860/JCDR/2015/.5447
Jyotsna Naresh Bharti, Poonam Rani, Vinay Kamal Prem, Narayan Agarwal
1. Senior Resident, Department of Pathology, Maulana Azad Medical College, New Delhi, India.
2. Senior Resident, Department of Pathology, Maulana Azad Medical College, New Delhi, India.
3. Director Professor, Department of Pathology, Maulana Azad Medical College, New Delhi, India.
4. Director Professor & HOD, Department of Surgery, Maulana Azad Medical College, New Delhi, India.
Correspondence Address :
Dr. Jyotsna Naresh Bharti,
Senior Resident, Department of Pathology, Maulana Azad Medical College, Bahadur Shah Zafar Marg, New Delhi 110002, India.
E-mail : jyotsnamamc@gmail.com
Abstract
Purpose: The aim of study is to evaluate angiogenesis using CD34, in estrogen, progesterone positive and negative breastcancer and to correlate the microvessel density with known histological prognostic factors, morphological type of breast carcinoma and lymph node metastasis.
Materials and Methods: Twenty eight untreated cases of breast cancer were included in the study and paraffin embedded sections were obtained from representative mastectomy specimen of breast cancer patient. The sections were stained with hematoxylin and eosin stain and immunohistochemistry was performed using CD34, estrogen, progesterone, cytokeratin and epithelial membrane antigen antibody. Angiogenesis was analysed using CD 34 antibody. For statistical analysis, cases were grouped into estrogen, progesterone positive and negative receptors.
Results: Mean microvessel density in ER-/PR-, ER-/ PR+, ER+/PR-, ER+/PR+ was 15.45, 14.83, 11, 10.89 respectively. A significant correlation was found between ER receptors and mean vascular density with p-value (< 0.05). A significant difference was observed in mean vascular density between the four groups comprising (p-value < 0.05). Infiltrating duct carcinoma (NOS) grade III has got the highest mean microvessel density (14.17) followed by grade II (12.93) and grade I (12.33).
Conclusion: Information about prognostic factors in breast cancer patients may lead to better ways to identify those patients at high risk who might benefit from adjuvant therapies.
Keywords
CD 34 antibody, ER, Immunohistochemistry, Microvessel density, Mastectomy
DOI: 10.7860/JCDR/2015/10591.5447
Date of Submission: Jul 16, 2014
Date of Peer Review: Sep 18, 2014
Date of Acceptance: Oct 27, 2014
Date of Publishing: Jan 01, 2015
Financial OR OTHER COMPETING INTERESTS: None.
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