Quantitative Buffy Coat Analysis-An Effective Tool for Diagnosing Blood Parasites
Published: April 1, 2014 | DOI: https://doi.org/10.7860/JCDR/2014/.4258
Nishat Hussain Ahmed, Jyotish Chandra Samantaray
1. Assistant Professor, Department of Laboratory Medicine, Delhi State Cancer Institute, Dilshad Garden, Delhi-110095, India.
2. Professor and Head, Department of Microbiology, All India Institute of Medical Sciences, Dilshad Garden, Delhi-110095, India.
Correspondence Address :
Dr. Nishat Hussain Ahmed,
Assistant Professor, Department of Laboratory Medicine, Delhi State Cancer Institute, Dilshad Garden, Delhi-110095, India.
Phone: 9990473406, E-mail: drnishathussain@rediffmail.com
Abstract
Quantitative buffy coat (QBC) analysis, which is based on principle of centrifugal stratification of blood components, is a well-known and a very sensitive technique which can be used for the detection of malarial parasites in peripheral blood. In our experience, this technique is also highly specific for doing speciation of malarial parasite in Indian set up. In addition, this technique was also found to be a sensitive and specific tool for diagnosing filariasis. Lastly, the cellular pattern of buffy coat in QBC, together with other non-specific findings, has many times aided in making correct diagnoses in difficult cases of visceral Leishmaniasis.
Keywords
Quantitative buffy coat, Malarial parasite, Filariasis, Visceral Leishmaniasis