Year :
2021
| Month :
October
| Volume :
15
| Issue :
10
| Page :
OC34 - OC37
Full Version
Antimicrobial Effect of 1,25 Dihydroxy Vitamin D on Vibrio cholerae and its Association with Serum 25-Hydroxy Vitamin D Level in Rural Elderly Women: An Experimental Study
Published: October 1, 2021 | DOI: https://doi.org/10.7860/JCDR/2021/49439.15571
Joyeta Ghosh, Aditi Nag Chaudhuri, Indranil Saha, Debnath Chaudhuri
1. Assistant Professor, Department of Dietetics and Nutrition, NSHM Knowledge Campus, Kolkata, West Bengal, India.
2. Professor, Department of Microbiology, Lady Brabourne College, Kolkata, West Bengal, India.
3. Scientist E, ICMR-Centre on Non-communicable Diseases, Kolkata, West Bengal, India.
4. Professor, Department of Biochemistry and Nutrition, All India Institute of Hygiene and Public Health, Kolkata, West Bengal, India.
Correspondence Address :
Dr. Joyeta Ghosh,
3D Madhukunja, Kolkata, West Bengal, India.
E-mail: joyetaghosh01@gmail.com
Abstract
Introduction: Aging is associated with a variety of functional, demographic and immunologic changes, responsible for increasing the incidence and severity of infectious diseases in the elderly.
Aim: To examine the association of serum 25-hydroxy vitamin D status with antimicrobial activity of cultured macrophages isolated within an exclusively elderly population cohort and also to examine the effect of in-vitro 1,25 hydroxy vitamin D supplementation in it.
Materials and Methods: This experimental study was conducted among 113 elderly women aged between 60 to 70 years of age, during the period of April 2014 to August 2018, at Amdanga block, North 24th Parganas, West Bengal, India. Their vitamin D status was assessed by the estimation of serum 25-hydroxy vitamin D and classified into three groups, sufficient group (60 subjects), insufficient group (27 subjects) and deficient group (26 subjects). Later their Peripheral Blood Mononuclear Cells (PBMC) were isolated and cultured from fresh blood of each and every study subject. Supplementation of 1,25 dihydroxy vitamin D {1,25(OH)2 D} was given selectively at a dose of 10×10-8 M for 72 hours in the culture media and were exposed to Vibrio cholerae and inducible nitric oxide synthase (iNOS), Superoxide Dismutase (SOD) activity and Bactericidal activity were screened. Statistical Package for the Social Sciences (SPSS) software, version 20.0 was used to perform statistical analysis.
Results: The sufficient group iNOS activity had significantly higher than the other two groups. Whereas in-vitro 1, 25(OH)2 D supplementation increases iNOS activity significantly among all three groups. As per the Colony Forming Unit (CFU) reduction rate against Vibrio cholerae infection the sufficient serum 25(OH) D consisting group had significantly (p-value=0.03) high CFU reduction rate than the other two groups. Although in-vitro 1, 25(OH)2 D supplementation had no significant impact on CFU reduction rate among all three groups.
Conclusion: Bacteria killing capacity of macrophages varies significantly with serum 25-hydroxy vitamin D level of the target population. Sufficient group macrophages always had better profile than other two groups. In-vitro 1, 25(OH)2 D supplementation increases iNOS and SOD activity significantly
Keywords
Inducible nitric oxide synthase, Macrophages, Superoxide dismutase, Supplementation
10.7860/JCDR/2021/49439.15571
Date of Submission: Mar 14, 2021
Date of Peer Review: Apr 27, 2021
Date of Acceptance: Aug 19, 2021
Date of Publishing: Oct 01, 2021
AUTHOR DECLARATION:
• Financial or Other Competing Interests: Funded by DST-INSPIRE Program Division, New Delhi; Department of Microbiology,
Lady Brabourne College, Department of Biochemistry and Nutrition, All India Institute of Hygiene and Public Health, Kolkata, India.
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. NA
PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Mar 15, 2021
• Manual Googling: Aug 10, 2021
• iThenticate Software: Sep 06, 2021 (10%)
ETYMOLOGY: Author Origin
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