Year :
2020
| Month :
October
| Volume :
14
| Issue :
10
| Page :
ZC21 - ZC25
Full Version
Comparative Evaluation of Antimicrobial
Efficacy, Depth of Penetration into Dentinal
Tubules and Effect on Microhardness of
Root Dentin by Sodium Hypochlorite,
Neem Extract and Gau Ark as Root
Canal Irrigants: An In-vitro Study
Published: October 1, 2020 | DOI: https://doi.org/10.7860/JCDR/2020/44988.14147
Dhanshri Shashikant Khade, ND Shashikiran, Sachin Gugawad, Namrata Gaonkar, Swapnil Taur, Savita Hadakar, Pooja N Mapara
1. Postgraduate Student, Department of Pedodontics and Preventive Dentistry, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.
2. Dean, Professor and Head, Department of Pedodontics and Preventive Dentistry, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.
3. Associate Professor, Department of Pedodontics and Preventive Dentistry, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.
4. Associate Professor, Department of Pedodontics and Preventive Dentistry, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.
5. Assistant Professor, Department of Pedodontics and Preventive Dentistry, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.
6. Assistant Professor,
Correspondence Address :
Dhanshri Shashikant Khade,
School of Dental Sciences, Krishna Institute of Medical Sciences Deemed to be University, Karad, Maharashtra, India.
E-mail: dhanshrikhade21@gmail.com
Abstract
Introduction: Disinfection of root canals can be done using intracanal irrigants. Sodium hypochlorite or NaOCL is amongst the most commonly used endodontic irrigants. Due to disadvantages of NaOCl, there is a need to identify a more biocompatible irrigant.
Aim: To evaluate the antibacterial efficacy, depth of penetration into dentinal tubules and effect on microhardness of root dentin when NaOCl, Neem extract, Gau ark and Normal saline used as an irrigant against Enterococcus faecalis.
Materials and Methods: Prepared Neem leaf extract, 3% NaOCl, Gau ark, Normal saline, Brain Heart Infusion (BHI) broth and BHI Agar were used in the present in-vitro study conducted from August-October 2019. Antimicrobial efficacy was measured by agar well diffusion method on 10 petriplates. After access opening and biomechanical preparation, each tooth sample was irrigated with 5 mL of irrigant. Samples were decoronated and roots were split along the longitudinal axis to check penetration depth of irrigants under stereomicroscope for 40 teeth samples. After preparation of 40 teeth sample, 2 mm dentin discs prepared were embedded in acrylic resin. Microhardness was checked under Vickers Microhardness (VHN) tester after samples were irrigated. Statistical Analysis of Variance and Tukey post-hoc test were used.
Results: Zones of inhibition were highest with NaOCl (7.66 mm). Irrigants penetration depth into dentinal tubules was significant with NaOCl with average value 0.35 mm. Microhardness of root dentin of saline was 145.90 VHN, followed by Neem 136.59 VHN which was then followed by Gau ark 123.05 VHN hence, Neem has lesser effect on microhardness value as compared to NaOCl and Gau ark. There was statistically significant difference among four groups regarding antimicrobial efficacy, depth of penetration into dentinal tubules and microhardness of root dentin.
Conclusion: Neem extract showed significant antimicrobial property as compared to Gau ark and normal saline, good penetration depth and less effect on microhardness of root dentin. Herbal irrigant like Neem extract can be used as a root canal irrigant
Keywords
Brain heart infusion agar, Irrigation, Stereomicroscope, Vickers hardness test
DOI: 10.7860/JCDR/2020/44988.14147
Date of Submission: May 11, 2020
Date of Peer Review: Jun 22, 2020
Date of Acceptance: Aug 19, 2020
Date of Publishing: Oct 01, 2020
AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? NA
• For any images presented appropriate consent has been obtained from the subjects. NA
PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: May 11, 2020
• Manual Googling: Aug 19, 2020
• iThenticate Software: Sep 29, 2020 (21%)
ETYMOLOGY: Author Origin
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