Year :
2020
| Month :
October
| Volume :
14
| Issue :
10
| Page :
BC19 - BC23
Full Version
Effect of Cigarette Smoking on Selected Antioxidant Enzymes and Oxidative Stress Biomarkers
Published: October 1, 2020 | DOI: https://doi.org/10.7860/JCDR/2020/45948.14138
Basant Joshi, Sangeeta Singh, Preeti Sharma, Tapan Mohapatra, Pradeep Kumar
1. PhD Scholar, Department of Biochemistry, Santosh Medical College, Ghaziabad, Uttar Pradesh, India.
2. Associate Professor, Department of Biochemistry, Government Medical College, Haldwani, Uttarakhand, India.
3. Associate Professor, Department of Biochemistry, Santosh Medical College, Ghaziabad, Uttar Pradesh, India.
4. Professor and Head, Department of Biochemistry, Santosh Medical College, Ghaziabad, Uttar Pradesh, India.
5. Professor, Department of Biochemistry, Santosh Medical College, Ghaziabad, Uttar Pradesh, India.
Correspondence Address :
Preeti Sharma,
Santosh Medical College, Ghaziabad, Uttar Pradesh, India.
E-mail: prcdri2003@yahoo.co.in
Abstract
Introduction: Cigarette Smoking (CS) is the single greatest preventable cause of disease and death and is rich in Reactive Oxygen and Nitrogen Species (ROS and RNS). These can cause the production of other free radicals, which, in turn, initiate lipid peroxidation and cause several diseases. Free radical scavenger enzymes namely Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) represent the enzymatic part that have the ability to inhibit oxidative stress by scavenging the highly destructive free radicals.
Aim: To study the effect of CS on selected antioxidant enzymes and oxidative stress biomarkers.
Materials and Methods: A case control study was conducted from September 2016 to September 2019 in which total of 284 healthy (without any systemic diseases) cigarette smokers (cases) in the age group of 18-60 years compared with age and sex matched 284 nonsmokers (controls) were included in the study. Estimation of serum 8-hydroxydeoxyguanosine (8-OHdG) by Enzyme Linked Immunosorbant Assay (ELISA), Malondialdehyde (MDA) by Thiobarbuturic Acid Reactive Substances (TBARS), SOD by water soluble tetrazolium salt 1, GPx and CAT by colorimetric method. The analysis was carried out using the SPSS 19.0.2 program for windows. Unpaired t-test and one-way ANOVA were used to analyse all the data for statistical significance.
Results: The mean Serum MDA and 8-OHdG levels were significantly raised 7.47±1.84, 63.41±22.44 as compared to nonsmokers (3.90±1.03, 40.04±20.14) and serum SOD, Gpx and CAT levels were decreased 62.55±19.97, 44.45±16.60 and 12.92±10.16 in cigarette smokers as compared to nonsmokers 274.04±68.37, 208.56±75.63 and 127.82±18.68, respectively. These differences were also found to be statistically significant in cigarette smokers according to duration and number of cigarette smoked at the level of <0.05.
Conclusion: Cigarette Smoking, especially long-term smoking may leads to significant changes in the enzymatic antioxidant defense systems of smokers. Discontinuation of smoking and general awareness needs to be created to minimise the risk of smoking related diseases.
Keywords
Catalase, Cigarette smokers, Glutathione peroxidase, Serum oxidase dismutase
DOI: 10.7860/JCDR/2020/45948.14138
Date of Submission: Jul 17, 2020
Date of Peer Review: Jul 29, 2020
Date of Acceptance: Sep 12, 2020
Date of Publishing: Oct 01, 2020
AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? No
• For any images presented appropriate consent has been obtained from the subjects. NA
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• Plagiarism X-checker: Jul 18, 2020
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ETYMOLOGY: Author Origin
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