CBNAAT Co-Testing of Sputum and BAL Fluid with Sputum Microscopy: May it Halt the March of Tuberculosis !Correspondence Address :
Dr. Dibakar Haldar,
Associate Professor, Department of Community Medicine, Anandapally, Sitko Road, Duttapara, Baruipur,
Kolkata-700144, West Bengal, India.
Introduction: Growing concern for Tuberculosis (TB) epidemic forces World Health Organization (WHO) and government of India (GOI) to incorporate newer rapid and highly specific diagnostic test like Cartridge Based Nucleic Acid Amplification Test (CBNAAT).
Aim: To find the usefulness of CBNAAT in increasing Acid Fast Bacilli (AFB) positive patient pool over and above the yield of traditional sputum microscopy.
Materials and Methods: The cross-sectional survey was conducted in the Department of Respiratory Medicine, Nilratan Sircar Medical College and Hospital (NRSMCH), Kolkata, India. The study involved 94 smear negative TB suspects referred from other health facilities as well as diagnosed by the department itself. After collecting baseline information like age, sex, previous history of TB and its treatment by interview and scrutinizing records using predesigned questionnaire, the patients were put on sputum CBNAAT and Broncho-Alveolar Lavage (BAL)-CBNAAT testing. Data were analysed by estimating mean, Standard Deviation (SD), proportion and using independent t-test, chi-square test.
Results: Overall, average age of participants was 44.7±15.3 (mean±SD) years. Male-female ratio was 1:2.8. Altogether 44.7% patients were detected sputum positive out of which 34.0% were detected only by sputum CBNAAT and another 10.7% detected when BAL-CBNAAT testing was used among the negatives yielded from sputum CBNAAT only. These differences were statistically significant.
Conclusion: Utility of CBNAAT over and above traditional diagnostic methods was reaffirmed. With added advantage of detecting MDR cases simple, sensitive, speedy and automated CBNAAT seems new mile stone in ‘Stop TB’ strategy and needs utilised to its highest potentiality through monitoring and supervision.
Drug resistant tuberculosis, Lavage, Nucleic acid amplification, Sputum microscopy
Amiya Kumar Dwari, Sumanta Jha, Dibakar Haldar, Bisanka Biswas,
Sanjay Kumar Saha, Partha Pratim Roy, et al.. CBNAAT CO-TESTING OF SPUTUM AND BAL FLUID WITH SPUTUM MICROSCOPY: MAY IT HALT THE MARCH OF TUBERCULOSIS !. Journal of Clinical and Diagnostic Research [serial online] 2018 June [cited: 2018 Sep 19 ]; 12:LC22-LC24. Available from
Date of Submission: Jan 25, 2018
Date of Peer Review: Mar 27, 2018
Date of Acceptance: Apr 10, 2018
Date of Publishing: Jun 01, 2018
FINANCIAL OR OTHER COMPETING INTERESTS: None.
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