Co-infection of Dengue and Leptospirosis in Patients Presenting to a Tertiary Care Hospital with Acute Febrile Illness: A Cross-sectional StudyCorrespondence Address :
Dr. Priyadarshini Shanmugam,
No 9/5, 7th Cross Street, Karpagam Gardens, Adyar, Chennai-600020, Tamilnadu, India.
E-mail: priyadarshini0018@ gmail.com
Introduction: Dengue and Leptospirosis are emerging important public health problems in India. While Dengue is a vector borne viral infection transmitted by Aedes mosquitoes, Leptospirosis is a zoonosis caused by pathogenic Leptospira. The incidence of both these infections increases during the rainy season. Due to simultaneous transmission of both these infections during rainy season, co-infections can occur. These mixed infections can change the clinical spectrum of disease to more fulminant course and presents a diagnostic and therapeutic challenge to the treating physicians.
Aim: To determine the prevalence of Dengue and Leptospira co-infection by Dengue NS1 antigen, IgM and IgG ELISA, Leptospira PCR and Leptospira IgM ELISA.
Materials and Methods: This was a cross-sectional study done over a period of one year at Chettinad Hospital and Research Institute. Symptomatic patients belonging to all age groups with fever of 7 days duration were included in the study. Blood (10ml) was collected from 100 febrile patients by using aseptic techniques, serum separated by centrifugation and then screened for both infections.
Results: Dengue infection was found to be positive in 29 patients (29%) and Leptospirosis was found to be positive in 18 patients (18%). Four (4%) patients had a co-infection of dengue and leptospirosis. Among the 29 dengue positive patients, NS1 antigen was positive in 22 (75.86%), anti-dengue IgM was positive in 15 (51.72%) and anti-dengue IgG was positive in 8 (27.58%) patients. Dengue RT-PCR was found to be positive in 20 patients (68.96%). Among the 18 Leptospira positive patients, Leptospira IgM and Leptospira PCR were positive in 8 (44.44%) & 10 (55.55%) patients respectively. Of the co-infected patients, 75% were found to be positive for Dengue NS1, RT-PCR and Leptospira PCR and 25% was found to be positive for anti-dengue IgM and Leptospira IgM antibodies.
Conclusion: Co-infections may result in illness with overlapping signs and symptoms and enhanced ability to diagnose it early in illness with robust diagnostic methods which will help guide the physicians.
Laboratory diagnosis, Molecular methods, Tropical fevers
Anitha Ravindar, Priyadarshini Shanmugam. CO-INFECTION OF DENGUE AND LEPTOSPIROSIS IN PATIENTS PRESENTING TO A TERTIARY CARE HOSPITAL WITH ACUTE FEBRILE ILLNESS: A CROSS-SECTIONAL STUDY. Journal of Clinical and Diagnostic Research [serial online] 2018 June [cited: 2018 Sep 19 ]; 12:DC05-DC09. Available from
Date of Submission: Feb 23, 2018
Date of Peer Review: Mar 15, 2018
Date of Acceptance: May 08, 2018
Date of Publishing: Jun 01, 2018
FINANCIAL OR OTHER COMPETING INTERESTS: None.
- Emerging Sources Citation Index (Web of Science, thomsonreuters)
- Index Copernicus ICV 2016: 132.37
- Academic Search Complete Database
- Directory of Open Access Journals (DOAJ)
- Embase & EMbiology
- Google Scholar
- HINARI Access to Research in Health Programme
- Indian Science Abstracts (ISA)
- Journal seek Database
- Popline (reproductive health literature)