Intratubular Penetration and Push-out Bond Strength of AH Plus, GuttaFlow 2 and GuttaFlow Bioseal Sealers: An In-vitro Study
Published: June 1, 2024 | DOI: https://doi.org/10.7860/JCDR/2024/70494.19565
Sangham Madakwade, Chetana Sachin Makade, Pratima Shenoi, Mohit Gunwal, Simran Gupta
1. Postgraduate Student, Department of Conservative Dentistry and Endodontics, VSPM Dental College and Research Centre, Nagpur, Maharashtra, India.
2. Associate Professor, Department of Conservative Dentistry and Endodontics, VSPM Dental College and Research Centre, Nagpur, Maharashtra, India.
3. Professor and Head, Department of Conservative Dentistry and Endodontics, VSPM Dental College and Research Centre, Nagpur, Maharashtra, India.
4. Associate Professor, Department of Conservative Dentistry and Endodontics, VSPM Dental College and Research Centre, Nagpur, Maharashtra, India.
5. Postgraduate Student, Department of Conservative Dentistry and Endodontics, VSPM Dental College and Research Centre, Nagpur, Maharashtra, India.
Correspondence
Dr. Chetana Sachin Makade,
Associate Professor, Department of Conservative Dentistry and Endodontics, Ranjeet Deshmukh Dental College and Research Centre, Hingna Road, Digdoh Hills, Nagpur-440019, Maharashtra, India.
E-mail: makade.chetana@gmail.com
Introduction: The root canal obturating material provides a three-dimensional fluid-impervious seal. Root canal sealers play a major role in providing a seal between the core material and the tooth for the long-term success of endodontic treatment.
Aim: To evaluate the intratubular penetration and Bond Strength (BS) and push-out BS of AH Plus, GuttaFlow 2, and GuttaFlow Bioseal sealers.
Materials and Methods: The in-vitro study was conducted in the Department of Conservative Dentistry and Endodontics at Ranjeet Deshmukh Dental College and Research Centre, Nagpur, Maharashtra, India, from October 2019 to April 2021. A total of 75 human mandibular first premolars were decoronated at the Cemento-enamel Junction (CEJ) with a standardised length of 16 mm. The root canal was prepared using the crown-down technique with HyFlex Electrical Discharge Machining (EDM) rotary files and divided into three groups: Group-I: AH Plus, Group-II: GuttaFlow 2, and Group-III: GuttaFlow Bioseal. 0.1% Rhodamine B dye was added to the sealer and obturated using the single cone technique. These samples were transversely sectioned into beams at the middle third of the root. A confocal laser scanning microscope was used to evaluate the depth of sealer penetration in the dentinal tubule, and a Universal Testing Machine was utilised to measure the push-out BS (MPa). The recorded data were subjected to statistical analysis {one-way Analysis of Variance (ANOVA) test, Tukey’s post-hoc test, and Pearson’s correlation coefficient}.
Results: The results obtained indicated that there was a significant difference in the depth of penetration and push-out BS of AH Plus and GuttaFlow 2 sealers (p<0.0001). However, there was no significant difference found between AH Plus and GuttaFlow Bioseal sealers (p=0.206). In addition, there was no significant difference in the correlation between intratubular penetration and push-out BS of the sealers (negative correlation).
Conclusion: Considering the results, the GuttaFlow Bioseal sealer was found to have comparable clinical performance with AH Plus sealer; hence, it can be used as an acceptable root canal sealer.
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