Original article / research
Comparative Evaluation of the Disinfection Efficacy of Herbal Formulation, 2% Glutaraldehyde, and Electrolysed Oxidising Water on Clinically Derived Polyvinyl Siloxane Impressions: An In-vivo Experimental Study
ZC58-ZC63
Correspondence
Dr. Srushti Ghagas,
Room No. 205, Department of Prosthodontics, Ranjeet Deshmukh Dental College, Digdoh Hills, CRPF Gate, Hingna Road, Nagpur-440019, Maharashtra, India.
E-mail: srushtighagas05@gmail.com
Introduction: Dental impressions are frequently contaminated with saliva, blood and microorganisms, posing a risk of cross-infection. Polyvinyl Siloxane (PVS) impressions require effective disinfection without compromising material properties. Although glutaraldehyde is widely used, concerns regarding toxicity and environmental impact have encouraged the exploration of safer alternatives such as Electrolysed Oxidising Water (EOW) and herbal formulations. However, limited comparative evidence exists regarding their antimicrobial efficacy on clinically derived PVS impressions.
Aim: To compare the antimicrobial efficacy of herbal formulation (HiOra™), 2% glutaraldehyde, and EOW on clinically derived PVS impressions.
Materials and Methods: This in-vivo experimental study was conducted in the Department of Prosthodontics, Ranjeet Deshmukh Dental College and Research Centre, Nagpur, Maharashtra, India, from December 2023 to April 2025. Eleven dentate subjects were included, yielding a total of 44 samples. The inclusion criteria were healthy dentulous subjects aged 18-45 years with no active oral lesions. Four samples from each impression were allocated to Group A (control), Group B (HiOra™), Group C (2% glutaraldehyde), and Group D (EOW). Samples were immersed for 10 minutes. Aerobic and anaerobic Colony Forming Units (CFUs) were quantified. Demographic parameters such as age and gender of the participants were recorded. Statistical analysis was performed using the Kruskal-Wallis test followed by post-hoc Bonferroni test, with significance set at p<0.05.
Results: Significant intergroup differences were observed (aerobic: p=0.003; anaerobic: p<0.001). Mean aerobic log CFU/mL values were 1.02±0.73 (control), 0.80±0.81 (HiOra™), 0.36±0.51 (glutaraldehyde), and 0.00±0.00 (EOW). EOW showed significantly lower aerobic counts compared to control (p=0.003) and HiOra™ (p=0.046), and was comparable to glutaraldehyde (p=1.000). Mean anaerobic log CFU/mL values were 2.67±1.09, 1.26±1.27, 0.72±1.40, and 0.09±0.30, respectively, with EOW demonstrating near-complete reduction.
Conclusion: EOW demonstrated the highest antimicrobial efficacy, followed by 2% glutaraldehyde, while the herbal formulation showed the least effectiveness. EOW may serve as a promising eco-friendly alternative for routine prosthodontic disinfection.