Hypoglycemic Effect of 70% Ethanolic Extract of Tinosporacrispa L. (Bratawali) Stem from Indonesia in Wistar Rat Induced by Alloxan FF01-FF03
Faculty, Department of Medicine, Universitas Muhammadiyah, Surakarta, Indonesia.
E-mail: Em.Sutrisna@ums.ac.id; email@example.com
Introduction: Indonesian people often use Bratawali (Tinosporacrispa L.) to treat Diabetes Mellitus (DM) empirically. Based on literature, the active compound in the plant is thought to initiate insulin secretion by modulating the ß-cell of Ca2+ concentration.
Aim: The aim of the present study is to evaluate hypoglycemic effect 70% ethanolic extracts of Tinosporacrispa L. (EETC) in Wistar rats induced by alloxan.
Materials and Methods:Twenty five rats were divided into 5 groups. The group I was negative control treated by aqua bidest, group II was positive control treated by glibenclamide at dose of 0.63/kg bw/day, group III was treated by EETC at dose of 500 mg/kgbw/day, group IV was treated by EETC at dose of 1000 mg/kgbw/day and group V was treated by EETC at dose of 2000 mg/kgbw/day. The fasting Blood Glucose Level (BGL) of all rats were measured and then they were injected by alloxan at dose of 150 mg i.p. On fourth day BGL were re-measured. All rats with BGL>200 mg/dL were treated by extract/medicine appropriated their group. This treatment was for ten days. The Fasting BGL between groups was analysed using ANOVA followed by LSD.
Results: The results of this research shows that the EETC at dose of 500 mg/kgbw/day and 2000 mg/kgbw/day can reduce fasting BGL in Wistar rats induced by alloxan significantly (p<0.05). The percentage of reduction of fasting BGL at dose of 500 mg/kgbw/day and 2000 mg/kgbw/day are 44.78±5.27 and 43.12±4.25 respectively. EETC at dose of 500 and 2000 mg/kg bw/day allegedly able to repair the pancreatic islet cell.
Conclusion: It is concluded that the EETC reduce fasting BGL in Wistar rats induced by alloxan.