Oxidised LDL and Serum Paraoxanase Activity in Ischemic Stroke Patients: A Case Control Study BC12-BC15
Dr. Geetha Ananthashenoyi,
Professor and Head, Department of Biochemistry, Government Medical College, Kottayam-686008, Kerala, India.
Introduction: Oxidative stress by producing lipid peroxidation products like Oxidised Low Density Lipoprotein (LDL) acts as independent risk factor in ischaemic stroke. When lipid peroxidation overwhelms antioxidative defense mechanism; it results in enhanced formation of oxidized LDL which promotes atherosclerosis. Human serum Paraoxanase (PON 1) which is an ester hydrolase synthesized in the liver prevents the oxidative modification of LDL.
Aim: To investigate the level of oxidized LDL as oxidative stress marker and paraoxonase activity as an antioxidant enzyme in ischaemic stroke patients and also the relation between Oxidized-Low Density Lipoprotein (Ox-LDL) level and Computed Tomography (CT) findings in ischaemic stroke patients.
Materials and Methods: The subjects in this study comprised of 40 ischaemic stroke patients and 40 age and gender matched controls. Fasting plasma glucose, serum paraoxanase activity, plasma oxidised LDL level, fasting lipid profile were determined. Data was analysed using Statistical Package for Social Sciences (SPSS) version 16.0.
Results: Ischaemic stroke patients had significantly lower paraoxanase activity (<53 kU/L), elevated levels of oxidized LDL (>2.26 Âµg/ml), total cholesterol (>200 mg%) and fasting plasma glucose levels (>126 mg%).
Conclusion: Oxidised LDL level >2.26 Âµg/ml, paraoxanase activity <53 kU/L and FBS >126 mg% were independent risk factors for stroke. Significantly higher levels of oxidized LDL were seen in patients with infarct in anterior circulation. This study implies that raised oxidized LDL level and low serum paraoxanase activity are independent risk factors of ischaemic stroke in patients with no other risk factors.