Analysis of Multiple Myeloma by FTIR Spectroscopy Coupled with Statistical Analysis 1001-1007
Surapaneni K.M. (MRSC, FAGE)
Department of Biochemistry,
Saveetha Medical College & Hospital,
Faculty of Medicine, Saveetha University,
Saveetha Nagar, Thandalam,
Chennai - 602 105, Tamilnadu, India.
Background: Myeloma is a disorder in which malignant plasma cells accumulate in the bone marrow and produce excess immunoglobulin that leads to many complications.
Aim: The aim of the present work was to analyze the difference in the nature of the immunoglobulin protein in healthy human blood plasma and myeloma which was infiltrated by human blood plasma, by employing Fourier Transform Infra Red (FTIR) Spectroscopy which was coupled with statistical analysis.
Materials and Methods: An infrared spectrum was recorded in the mid frequency region, in the 4000 – 450 cm-1 range for normaland various types of myeloma which were infiltrated with blood plasma samples, namely IgA and IgG. The proposed method was then quantitatively analyzed by intensity ratio calculation among the spectral absorption peaks and by the Independent t-test.
Results: In Ig A myeloma, the proliferation is more when compared to the normal healthy controls. On contrary, in Ig G myeloma, there observed a reduction in the Ig G immunoglobulins.
Conclusion: From the results, it has been established that IR spectroscopy has the potential to be an efficient and effective diagnostic tool in the analysis of blood.