Most problems that threaten the health of reproductive system, especially testicular function, are associated with free radical-induced oxidative stress. Life-threatening attacks of free radicals may cause arterial occlusion and serious damage to the reproductive system cells, and consequently defects in spermatogenesis. In other words, increased production of free radicals and per oxidants as well as weakened antioxidant defense system lead to oxidative stress . Therefore, it is necessary to create a balance between produced free radicals and its metabolism for appropriate function of testicular cells, because if the testicular biological system fails to detoxify or repair the adverse effects of free radicals, the cells and tissue are damaged seriously . In this regard, antioxidants can avoid this damage by counteracting free radicals or preventing their formation in the testicular cells. It is noteworthy that a part of the body’s antioxidant defense system, called preventive antioxidant system, is related to antioxidant enzymes such as Superoxide Dismutase (SOD), catalase, and Glutathione Peroxidase (GPX) [3-6]. These enzymes avoid oxidation by decreasing the rate of chain formation. These antioxidants can stop an oxidation chain forever by finding primer free radicals. In addition, through stabilizing metal radicals such as copper and iron, they can inhibit their oxidation, preventing various diseases [3-5]. Another part of the body’s antioxidant defense system is called scavenging antioxidant system. These antioxidants delete Reactive Oxygen Species (ROS) produced in the body’s different tissues to prevent peroxidation of plasma membrane lipids . Vitamins such as E and C are some examples of these antioxidants. These antioxidants neutralize free radicals and prevent them from damaging the cell and tissues. Given that the antioxidants produced by the body are not able to neutralize all free radicals, then use of antioxidant supplements can play an important role in increasing the body’s capacity to fight free radicals [7,8]. In this article other than presenting the role of oxidative stress on testicular function, the plants with antioxidant activities, which have positive effects on testicular function, are reviewed.
The Effect of Oxidative Stress on Sperm Morphological Characteristics
Damage to sperm morphological characteristics: Various parameters of sperm such as count, motility, and morphology are significantly susceptible to free radicals and hence free radicals can reduce sperm fertility . The free radical-induced oxidative stress contributes significantly in producing and increasing abnormal sperm and decreasing sperm count and transformation and fragmenting sperm DNA. These changes in sperm DNA result in infertility. In this regard, incubation of spermatozoa under high oxygen pressure reduces the rate and motility of sperm; however, adding catalase to the culture medium prevents this effect . Increased production of Hydrogen Peroxide (H2O2) by spermatozoa under high oxygen pressure may be a factor for reducing sperm motility . Moreover, the Reactive Oxygen Species (ROS) produced by leukocytes or spermatozoid have fatal effects on sperm function in infertile men. Thus, the ROS produced in the sperm must be inactivated continuously such that ROS concentration constantly remains low enough to allow for the normal performance of cells, because a lack of balance between the production and elimination of ROS causes oxidative stress in sperm and subsequently reduces fertility potential. Researchers believe that the sperm is more susceptible to oxidative stress than other cells due to the limited amount of cytoplasm in a mature sperm and the concentration of ROS-suppressing antioxidants in the sperm as well as high levels of unsaturated fatty acids in the sperm structure . In addition, according to the particular morphology of sperm, antioxidant enzymes in the sperm fail to protect plasma membrane surrounding acrosome and tail. Therefore, the health and fertility of sperm are greatly dependent on the availability of the antioxidants, which mostly is related to the antioxidant systems in the seminal plasma. If the antioxidants are separated from the semen for any reason such as washing, sperm becomes susceptible to oxidative damage. Researchers believe that sperms can fight oxidative stress conditions mainly due to the antioxidant properties of semen  such that ROS generated in the semen is constantly deactivated by seminal antioxidants under normal conditions. Therefore, one of the reasons for establishment of oxidative stress conditions is the imbalance between the production of ROS and its inactivation by antioxidants of semen.
Lipid peroxidation of sperm plasma membrane: Lipid peroxidation in the cell membrane can disrupt fluidity and permeability of cell membranes and damage all cells. In other words, when the cell membranes are damaged by free radicals, their protective cell is lost and thus the total cell is exposed to risk. In this regard, increased production of ROS induces lipid peroxidation in spermatozoa, which has two important effects: 1) Reducing sperm combination with oocyte; 2) Increasing spermatozoa ability to bind to the transparent area (zona placida) . As well, lipid peroxidation caused abnormality in the middle section of sperm and loss of acrosome capacity of fertilization Malondialdehyde (MDA) molecules cause asymmetric distribution of lipid membrane components by penetrating into the cell membrane structure. Notably, the rate of lipids peroxidation is determined according to the resulting product of secondary failure of primary lipid hydro peroxides . MDA is produced due to the degradation of the peroxides of unsaturated fatty acids. It is used as a marker (biomarker) to determine the rate of oxidative damage to lipids that differs depending on biotic and abiotic stress. This issue has been one of the used indicators in the studies on lipids peroxidation in humans and animals. It is noteworthy that currently, the damage caused by lipid peroxidation is the most important factor for testicular dysfunction .
Damage to DNA sperm: It is important to protect integrity and accuracy of DNA in the sperm nucleus to transfer genetic material completely from one generation to another, because genetic material disorder causes defective transmission of genetic information to embryo. Oxidative stress causes increase in DNA breakdown . Besides that, evidence indicates that fragmentation of DNA is, commonly seen in infertile people’s spermatozoa, is due to the ROS high concentration in the sperm . In a study on DNA samples in people with teratozoospermia, the DNA damage rate was higher in the samples of people with spermatozoospermia than in those of healthy people; moreover, this damage was demonstrated to be mainly due to the amount of ROS produced by these sperms which can be the cause of infertility in people with spermatozoospermia. Therefore, a main reason for infertility is believed to be excessive production of ROS or decreased antioxidant capacity in semen that causes oxidative stress conditions and ultimately decrease in sperm motility, increase in sperm death, and fragmentation of DNA . A study reported that the oxidative damage to DNA is 100 times higher in infertile men than in fertile men. Relevantly, ROS concentration is very high in the sperm of the men whose wives have previous abortion; therefore, increased oxidative stress in these people’s testicles leads to destruction of sperm membrane and hence damage to DNA. This can be associated with abortion among such people’s wives . In this regard, semen has been reported to contribute significantly to maintaining sperm DNA’s health such that gonads are thought to be one of the main sources of sperm-protective antioxidants . In a study, the effect of Hamster gonads removal was investigated on sperm health. The findings demonstrated that removing the gonads caused increase in damage to hamster DNA . Therefore, gonads can be considered the main source of antioxidants in the semen.
Factors for Inducing Testicular Oxidative Stress
Despite the role of antioxidant agents in protecting testicular function throughout spermatogenesis process, a wide range of internal and external factors can cause disturbance in antioxidant defense and subsequently induce oxidative stress. Some of these factors are as follows:
Testicular torsion: Testicular torsion is a rare disease, which is mainly seen in mature men and leads to disturbed testicular blood flow. The incidence rate of testicular torsion is estimated to be one per 158 people at the age of 25 years, which is associated with decreased quality of ejaculation in over 35% of this population . If testicular torsion is not treated within 3-4 hours after incidence, it can lead to permanent testicle shrinkage . Long-term testicular torsion results in testicular ischaemia (decrease in testicular blood flow), increase in levels of oxidative stress in the testicles of the same side, increase in production of NO and H2O2, formation of lipids peroxidation, accumulation of isoprostane, decrease in antioxidant enzymes, and increase in apoptosis rate . Even, short periods of ischaemia, for three hours or shorter, can lead to high levels of testicular oxidative stress, decrease in testicular glutathione level, and spermatogenesis-induced disorder . The tissue damage due to testicular torsion can be significantly reduced by pretreatment with certain antioxidants such as selenium, resveratrol, L-carnitine, caffeic acid phenethyl ester and Allium sativum extract .
High testicular temperature: Any factors that cause abnormal increase in testicular temperature are associated with testicular oxidative stress. Besides that, increased testicular temperature is associated with decreased SOD and function of catalase . The exposure of sperm germ cells to increased temperature consequently increases the H2O2 level and is associated with increased rate of apoptosis. Therefore, increase in the catalase level can largely prevent cell death, as they decrease the H2O2 level .
Varicocele: Varicocele, dilation of spermatic vein in the left testicle, is associated with increased rate of male infertility . Varicocele is associated with induction of oxidative stress through disturbing spermatogenesis process via related mechanisms. In some clinical trials, the incidence of varicocele has been demonstrated to be associated with excessive production of ROS by sperm, high levels of damage to DNA in these cells, and drainage of seminal plasma antioxidants . These studies have demonstrated that varicocele causes induction of oxidative stress, which was confirmed in animal model. Varicocele was associated with testicular and seminal oxidative stress and decrease in testicular antioxidant capacity in mice [2,26]. It is noteworthy that varicocele causes increase in testicular blood flow and subsequently testicular temperature [21,27].
Diabetes: A study showed that experimentally inducing diabetes in animal models cause induction of oxidative stress by increasing the free radicals and subsequently disturbing testicular function thereby effecting fertility. Diabetes may contribute to male infertility by increasing the production of ROS and lipid peroxidation in testicles. In this regard, diabetes-induced oxidative stress causes DNA breakdown and increase in fetal mortality. These adverse effects can be repaired by use of certain antioxidants such as vitamin C, melatonin and taurine . The level of damage to DNA is more in the sperms of males with diabetes compared to those without diabetes .
Infection: Oxidative stress due to inflammation and inflammatory disease is a common predisposing factor for male infertility. Testicular infection causes a significant decrease in production of testosterone and disturbance in spermatogenesis . Analysis of microarray data on the genes expression in infertile men’s testicles indicates increased expression of inflammatory genes . A study showed that the oxidative stress induced by intraperitoneal administration of Lipopolysaccharide (LPS) caused stimulation of lipid peroxidation in Leydig cell membrane and considerable increase in steroid making and beta-hydroxyl dehydrogenase activity. Besides, this administration caused mitochondrial and Leydig cell dysfunction and specifically, inhibition of cholesterol transport activity .
Hyperthyroidism: Hyperthyroidism is associated with oxidative stress in mice testicles, an increase in lipid peroxidation and GSH level and induction of antioxidant enzymes. This oxidative stress appears to be due to increased mitochondrial activity and concurrent release of electrons from mitochondrial electron transport chain due to increased production of thyroxine . Now-a-days, the complications due to hyperthyroidism-induced oxidative stress can be repaired by melatonin, an important antioxidant; therefore, exacerbation of oxidation can be prevented . Clinical trials have shown that hyperthyroidism causes decline in seminal quality especially disturbance in sperm motility. Furthermore, hypothyroidism causes induction of oxidative stress . Therefore, it can be argued that normal testicular function is heavily dependent on thyroid function.
Imbalance among reproductive hormones: The conditions of the endogenous glands of the testicles can have an immediate effect on antioxidant activity of this organ. For example, treatment with cyclophosphamide and dimethane sulfanate can cause inhibition of expression of antioxidant enzymes such as glutathione peroxidase, SOD and catalase and decrease in testosterone concentration, disturbance in spermatogenesis and increase in cell apoptosis in testicles . In this regard, gonadotropinexogen can affect testosterone production inversely and cause inhibition of antioxidant enzymes expression; moreover, this hormone disturbs spermatogenesis process and causes cell death. When Leydig cells in rats are treated with human chorionic gonadotropin for the long-term, ROS are excessively produced in testicular tissue cells and subsequently lipid peroxidation is stimulated, the activity of antioxidant enzymes is reduced, the apoptosis of germinal cells is induced and spermatogenesis is disturbed .
Xenobytes effect: Xenobytes, including the residues of antibiotics, refers to the residues of the drugs and chemicals that are stored in the body in different ways in the long term. Although low amounts of xenobytes are less likely to cause damage, long-term accumulation of these substances can be associated with certain problems in the body. Recently, a wide spectrum of xenobytes has been demonstrated to induce testicular oxidative stress alongside suppressing antioxidant mechanism .
Being exposed to toxins: Environmental toxins can cause testicular oxidative stress and consequently disturbance in spermatogenesis. For example, treating mice with certain pesticides such as hexachlorocyclohexane caused a significant increase in testicular oxidative stress and hence damaged germ cells and apoptosis . Moreover, industrial pollutants such as 3,1-dinitrobenzene or nonylphenol exerted similar effects . Methoxyethanol, the glycol ether used in colours, brake oils and some other industrial pollutants as well as its main metabolite, methoxyacid, cause increase in testicular oxidative stress and atrohpy . Besides that, being exposed to high concentration of particular metals can cause oxidative stress. For example, a study on rats demonstrated that high concentration of iron increased oxidative stress and in contrast, use of antioxidants relieved oxidative stress in testicular tissue. Cadmium increases testicular oxidative stress, as well . Moreover, exposure to lead causes decline in testicular sperm output, increase in production of sperm ROS, decrease in epididymal sperm motility and increase in lipid peroxidation in mice [43,44]. Finally, it is worth mentioning that adoption of inappropriate lifestyle such as excessive use of alcohol or increased smoking can increase production of free radicals in all tissues and has been frequently associated with or contributed to male infertility .
Ionizing radiation: Testicular tissue is highly dependent on X-ray radiation; therefore, being exposed to X-ray can be associated with oxidative stress in testicular tissue . It should be noted that although all testicular cells have the same susceptibility rate to X-ray radiation, Sertoli and Leydig cells seem to be relatively resistant to X-ray, which can be reinforced by use of antioxidants [47,48].
Old age: A study demonstrated that in rats, as age increases, the expression of enzymatic and non-enzymatic antioxidants decreases, which led to increase damage due to oxidative stress. Besides that, the level of glutathione, an antioxidant, decrease in older mice . According to the findings of different studies, aging causes degenerative changes in testicular tissue and decline in sperm quality in rodents. In this regard, certain vacuoles are seen in the germinal cells of testicular tissue in laboratory mice with increase in age. In addition, the number of germinal cells decreased in mice testicles . Despite different studies conducted on the effect of age on increase in testicular oxidative stress, further research should be conducted to establish the association between aging, oxidative stress and testicular function.
Effective Antioxidants in Decreasing Testicular Oxidative Stress
As already mentioned, in addition to relying on the main, free radical-fighting enzymes, testicles are heavily dependent on antioxidant agents with low molecular weight to fight oxidative stress-induced complications. By nature, these factors are considered to be the scavengers or cleansers of free radicals.
Zinc: Zinc is a potent antioxidant agent and a main component of free radical-inhibiting free enzymes such as SOD. Moreover, this element, as a catalyst, can prevent lipid peroxidation through relocating or transferring metals including iron and copper . In a study, the rats fed with zinc-deficient diet exhibited decrease in antioxidant defense potential and concurrent increase in lipid peroxidation in testicular tissue .
Vitamins C and E: Vitamin E (α-tocopherol) is a potent, lipophilic antioxidant, which is vital to protect and maintain mammalian sperm. Besides that, this element contributes greatly to the activity of Sertoli cell lines and spermatocytes . Similarly, vitamin C (ascorbic acid) plays an important part in the process of spermatogenesis. As a result, vitamins C or E deficiency leads to induction of testicular oxidative stress and hence disturbs spermatogenesis and production of testosterone . In contrast, a study demonstrated that feeding with ascorbate caused stimulation of spermatogenesis and secretion of testosterone in healthy animals. In addition, use of vitamins C and E is highly effective to fight testicular oxidative stress due to exposure to oxidants such as arsenic, cadmium, endosulfan and alcohol and can considerably decrease the complications due to these substances. A study demonstrated that vitamin E was effective on testicular function through suppressing lipid peroxidation in testicular and mitochondrial microsomes and fighting adverse effects of oxidative stress due to exposure to certain agents such as ozone gas, iron overload, intense exercise, aflatoxin, cyclophosplamide and formaldehyde .
Selenium: Selenium is an essential mineral to protect the body against free radicals. This element protects important antioxidants in the body including vitamins C and E and decreases free radical-induced damage. Selenium plays a part in producing thyroid hormones and contributes to the function of this important gland. Selenium contributes greatly to fertility.
In the light of biologically important role of selenium especially in male reproductive system, people with high oxidative stress such as chronic disease (diabetes, cardiovascular disease and HIV), elderly people, alcoholics and smokers are recommended to use a selenium-rich meal. In this regard, an inverse correlation has been observed between seminal selenium level and sperm motility. In addition, selenium levels were significantly higher in fertile men’s semen than in infertile men’s . Use of vitamin E supplement and selenium considerably reduced Malondialdehyde (MDA) and improved sperm motility. Furthermore, selenium is useful to protect sperm DNA against oxidative stress and hence increase in motility and viability of sperm. According to these findings, selenium deficiency in rat’s diet caused damage to seminiferous tubules and decrease in sperm motility and count .
Melatonin and cytochrome C: Melatonin has two important characteristics that differentiate it from other oxidants. First, melatonin, as an antioxidant, shares one electron, rather than two electrons, in oxidation reaction. Therefore, free radicals are more likely to target melatonin. As a result, melatonin causes decrease in free radicals and oxidation rate. Secondly, melatonin is both water- and fat-soluble and can easily pass through testicular blood barrier to protect germinal epithelium. The melatonin level in seminal plasma is associated with weak sperm motility, leukocytospermia, varicocele and non-obstructive azoospermia in infertile men and consequently oxidative stress in male reproductive system . In addition, intraperitoneally administered melatonin has caused decrease in testicular oxidative stress after experimental induction of varicocele of the left side . Cytochrome C is another antioxidant that can effectively fight free radicals and special role has recently been confirmed in reducing testicular H2O2. Cytochrome C is a small protein, which is similar to coenzyme Q (ubiquinone) in terms of motility. The cytochrome C isoform is considered a potent apoptosis activator and plays a part in increasing the protective capacity of testicular tissue through eliminating damaged germ cells .
The Mechanism of Semen Antioxidant Protection
Semen contains antioxidant compounds that protect spermatozoa against oxidative stress and therefore, offset deficiency of sperm cytoplasmic enzymes. Semen contains a group of enzymatic antioxidants such as SOD, catalase, GPX and certain non-enzymatic antioxidants such as taurine, pyruvate, ureate, ascorbate, and α-tocopherol. Fertile men’s semen has a higher antioxidant capacity than infertile men’s . Seminal plasma contains three enzymatic antioxidants, SOD, catalase and GPX that prevent damage to cell structure and also the reduction of hydrogen peroxide into water and alcohol. Meanwhile, SOD with a comparably higher amount than other antioxidant enzymes leads to conversion of superoxide (NO2) anion into O2 and H2O2. Furthermore, this enzyme protects spermatozoa against O2 toxicity and lipid membrane against peroxidation. Catalase decomposes H2O2 into O2 and H2O. This enzyme also separates the superoxide anion produced by NADPH oxidase from neutrophils and protects spermatozoids against oxidative damage. In addition to containing enzymatic antioxidants, semen has a number of non-enzymatic antioxidants such as vitamins E and A, ascorbate, pyruvate, ubiquinole, glutathione, albumin, ureate, taurine, and hypotaurine, each of which plays a vital part in fighting oxidative stress . In this regard, certain amount (mM) of glutathione has been found in a number of cells. Glutathione reacts directly with ROS. Glutathione is a cofactor for GPX and protects mammalian cells against oxidative stress while reducing H2O2 and other peroxides. A study showed that 5 mM of glutathione exerts protective effects on sperm against freezing and is associated with enhanced sperm motility after freezing. Furthermore, this compound increases enzymatic activity in sheep semen . Vitamin E is the most abundant fat-soluble antioxidant that contributes greatly to seminal antioxidant system. Therefore, the activity of vitamin E, as a coenzyme for enzymatic reactions, is vital and helps to neutralize free radicals and ultimately reduce seminal oxidative stress. In addition, vitamin E or tocopherol protects intracellular and cell membrane unsaturated fatty acids against oxidative damage. Moreover, vitamin E activity is complementary to GPX activity, which facilitates the peroxides reduction in seminal cytoplasm.
Free radicals’ life-threatening attacks to the body’s different organs can cause arterial occlusion and induction of oxidative stress and subsequently causing serious damage to tissues. Meanwhile, testicular tissue is highly predisposed to activity of free radicals and oxidative stress due to several reasons including high cell division rate, cell competition for oxygen rate, low oxygen pressure due to weakened vessels as well as high levels of unsaturated fatty acids. Furthermore, since the body’s antioxidant system, including antioxidant enzymes such as SOD, catalase and GPX produced in the body is not able to neutralize all free radicals, the use of antioxidant supplements is recommended to fight adverse effects of oxidative stress, enhance spermatogenesis and increase enhance fertility.
. Halliwell B, Oxidative stress and neurodegeneration: where are we now? J Neurochem 2006 58:1634-36. [Google Scholar]
. Romeo C, Antonuccio P, Esposito M, Marini H, Impellizzeri P, Turiaco N, Hydrophilic vitamin E-like antioxidant reduces testicular ischemiare perfusion injury Urol Res 2004 32:367-71. [Google Scholar]
. Szabo C, Ischiropoulus H, Radi R, Peroxynitrite biochemistry, pathophysiology and development of therapeutics Nat Rev 2007 6:662-79. [Google Scholar]
. Nasri H, Rafieian-Kopaei M, Protective effects of herbal antioxidants on diabetic kidney disease J Res Med Sci 2014 19(1):82-83. [Google Scholar]
. Bahmani M, Mirhoseini M, Shirzad H, Sedighi M, Shahinfard N, Rafieian-Kopaei A, Review on promising natural agents effective on hyperlipidemia J Evid Based Complementary Altern Med 2015 20(3):228-38. [Google Scholar]
. Rafieian-Kopaie M, Baradaran A, Plants antioxidants: From laboratory to clinic J Nephropathol 2013 2(2):152-53. [Google Scholar]
. Pryor WA, Houk KN, Foote CS, Fukuto JM, Ignarro LJ, Squadrito GL, Free radical biology and medicine: it is a gas, man! Am J Physiol Regul Integr Comp Physiol 2006 291:R491-R511. [Google Scholar]
. Mates JM, Sanchez-Jimenez F, Antioxidant enzymes and their implications in pathophysiologic processes Front Biosci 1999 4:339-45. [Google Scholar]
. Saleh RA, Agarwal A, Oxidative stress and male infertility: from research bench to clinical practice J Androl 2002 23(6):737-52. [Google Scholar]
. Aitken RJ, Clarkson JS, Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa Reprod Fert 1987 81:459-69. [Google Scholar]
. Baker MA, Aitken RJ, The importance of redox reguy lated pathways in sperm cell biology Mol Cell Endocrinol 2004 216(1-2):47-54. [Google Scholar]
. Showell MG, Brown J, Yazdani A, Stankiewicz MT, Hart RJ, Antioxidants for male subfertility Cochrane Database Syst Rev 2011 (1):CD007411 [Google Scholar]
. Said TM, Agarwal A, Sharma RK, Thomas AJ, JrSikka SC, Impact of sperm morphology on DNA damage caused by oxidative stress induced by beta-nicotinamide adenine diy nucleotide phosphate Fertil Steril 2005 83(1):95-103. [Google Scholar]
. Peltola V, Mantyla E, Huhtaniemi I, Ahotupa M, Lipid peroxidation and antioxidant enzyme activities in the rat testis after cigarette smoke inhalation or administration of polychlorinated biphenyls or polychlorinated naphthalene’s J Androl 1994 15:353-61. [Google Scholar]
. World Health Organisation. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university press, 1999 [Google Scholar]
. Bennetts LE, Aitken RJ, A comparative study of oxidative DNA damage in mammalian spermatozoa Mol Reprod Dev 2005 71:77-87. [Google Scholar]
. Venkatesh S, Thilagavathi J, Kumar K, Deka D, Talwar P, Dada R, Cytogenetic, chromosome microdeletion, sperm chromatin and oxidative stress analysis in male partners of couples experiencing recurrent spontaneous abortions Arch Gynecol Obstet 2011 284(6):1577-84. [Google Scholar]
. Lewis SE, Sterling ES, Young IS, Thompson W, Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men Fertil Steril 1997 67(1):142-47. [Google Scholar]
. Anderson JB, Williamson RCN, Fertility after torsion of the spermatic cord Br J Urol 1990 65:225-30. [Google Scholar]
. Guimaraes SB, Aragao AA, Santos JM, Oxidative stress induced by torsion of the spermatic cord in young rats Acta Cir Bras 2007 22:30-33. [Google Scholar]
. Sarica K, Kupeli B, Budak M, Koşar A, Kavukçu M, Durak I, Influence of experimental spermatic cord torsion on the contralateral testis in rats: Evaluation of tissue free oxygen radical scavenger enzyme levels Urol Int 1997 58:208-12. [Google Scholar]
. Ahotupa M, Huhtaniemi I, Impaired detoxification of reactive oxygen and consequentoxidative stress in experimentally cryptorchid rat testis Biol Reprod 1992 46:1114-18. [Google Scholar]
. Ikeda M, Kodama H, Fukuda J, Shimizu Y, Murata M, Kumagai J, Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress Biol Reprod 1999 61:393-99. [Google Scholar]
. Fretz PC, Sandlow JI, Varicocele: Current concepts in pathophysiology, diagnosis, and treatment Urol Clin North Am 2002 29:921-38. [Google Scholar]
. Smith R, Kaune H, Parodi D, Madariaga M, Rios R, Morales I, Increased sperm DNA damage in patients with varicocele: Relationship with seminal oxidative stress Hum Reprod 2006 21:986-93. [Google Scholar]
. Asmis R, Qiao M, Rossi RR, Cholewa J, Xu L, Asmis LM, Adriamycin promotes macrophage dysfunction in mice Free Radic Biol 2006 41:165-74. [Google Scholar]
. Turner TT, Tung KSK, Tomomasa H, Wilson LW, Acute testicular ischemia results in germ cell-specific apoptosis in the rat Biol Reprod 1997 57:1267-74. [Google Scholar]
. Mallick C, Mandal S, Barik B, Bhattacharya A, Ghosh D, Protection of testicular dysfunctions by MTEC, aformulated herbal drug, in streptozotocin induced diabetic rat Biol Pharm Bull 2007 30:84-90. [Google Scholar]
. Agbaje IM, Rogers DA, McVicar CM, Insulin dependent diabetes mellitus: Implications for male reproductive function Hum Reprod 2007 22:1871-77. [Google Scholar]
. Reddy MM, Mahipal SV, Subhashini J, Reddy MC, Roy KR, Reddy GV, Bacterial lipopolysaccharide-induced oxidative stress in the impairment of steroidogenesis and spermatogenesis in rats Reprod Toxicol 2006 22:493-500. [Google Scholar]
. Allen JA, Diemer T, Janus P, Hales KH, Hales DB, Bacterial endotoxin lipopolysaccharide and reactive oxygen species inhibit Leydig cell steroidogenesis via perturbation of mitochondria Endocrine 2004 25:265-75. [Google Scholar]
. Husain K, Somani SM, Interaction of exercise training and chronic ethanol ingestion on testicular antioxidant system in rat J Appl Toxicol 1998 18:421-29. [Google Scholar]
. Sahoo DK, Roy A, Chattopadhyay S, Chainy GBN, Effect of T3 treatment on Ahotupa M, Huhtaniemi I. Impaired detoxification of reactive oxygen and glutathione redox pool and its metabolizing enzymes in mitochondrial and post-mitochondrial fractions of adult rat testes Indian J Exp Biol 2007 45:338-46. [Google Scholar]
. Mogulkoc R, Baltaci AK, Aydin L, Oztekin E, Tuncer I, Pinealectomy increases oxidant damage in kidneyand testis caused by hyperthyroidism in rats Cell Biochem Funct 2006 24:449-53. [Google Scholar]
. Krassas GE, Pontikides N, Deligianni V, Miras KA, A prospective controlled study of the impact of hyperthyroidism on reproductive function in males J Clin Endocrinol Metab 2002 87:3667-71. [Google Scholar]
. Zini A, Schlegel PN, Effect of hormonal manipulation on mRNA expression of antioxidantenzymes in the rat testis J Urol 2003 169:767-71. [Google Scholar]
. Gautam DK, Misro MM, Chaki SP, hCG treatment raises H2O2 levels and induces germ cell apoptosis in rat testis Apoptosis 2007 (in press) [Google Scholar]
. Ozyurt H, Pekmez H, Parlaktas BS, Oxidative stress in testicular tissues of rats exposedto cigarette smoke and protective effects of caffeic acid phenethyl ester Asian J Androl 2006 8:189-93. [Google Scholar]
. Samanta L, Chainy GB, Comparison of hexachloro cyclohexane induced oxidative stress in the testis of immature and adult rats Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 1997 118:319-27. [Google Scholar]
. Han X, Tu Z, Gong Y, Shen S, Wang X, Kang L, The toxic effects of nonylphenol on the reproductive system of male rats Reprod Toxicol 2004 19:215-21. [Google Scholar]
. McClusky LM, De Jager C, Bornman MS, Stage-related increase in the proportion of apoptotic germ cells and altered frequencies of stages in the spermatogenic cycle following gestational, lactational, and direct exposure of male rats to p-nonylphenol Toxicol Sci 2007 95:249-56. [Google Scholar]
. Koizumi T, Li ZG, Role of oxidative stress in single-dose, cadmium-induced testicular cancer J Toxicol Environ Health 1992 37:25-36. [Google Scholar]
. Hsu PC, Liu MY, Hsu CC, Chen LY, Leon-Guo Y, Lead exposure causes generation of reactive oxygen species and functional impairment in rat sperm Toxicology 1997 122:133 [Google Scholar]
. Marchlewicz M, Wiszniewska B, Gonet B, Baranowska-Bosiacka I, Safranow K, Kolasa A, Increased lipid peroixdation and ascorbic acid utilization in testis and epididymis of rats chronically exposed to lead Biometals 2007 20:13-19. [Google Scholar]
. Mattison DR, The effects of smoking on fertility from gametogenesis to implantation Environ Res 1982 28:410-33. [Google Scholar]
. Manda K, Ueno M, Moritake T, Anzai K, Alpha-lipoic acid attenuates x-irradiation-induced oxidative stress in mice Cell Biol Toxicol 2007 23:129-37. [Google Scholar]
. Lee K, Park JS, Kim YJ, Soo-Lee YS, Sook-Hwang TS, Kim DJ, Differential expression of Prx I and II in mouse testis and their up-regulation by radiation Biochem Biophys Res Commun 2002 296:337-42. [Google Scholar]
. Ahotupa M, Huhtaniemi I, Impaired detoxification of reactive oxygen and consequent oxidative stress in experimentally cryptorchid rat testis Biol Reprod 1992 46:1114-18. [Google Scholar]
. Syntin P, Chen H, Zirkin BR, Robaire B, Gene expression in Brown Norway rat Leydig cells: effects of age and of age-related germ cell loss Endocrinology 2001 142:5277-5285. [Google Scholar]
. Luo L, Chen H, Trush MA, Show MD, Anway MD, Zirkin BR, Aging and the Brown Norway rat Leydig cell antioxidant defense system J Androl 2006 27:240-47. [Google Scholar]
. Bray TM, Bettger WJ, The physiological role of zinc as an antioxidant Free Radic Biol Med 1990 8:281-91. [Google Scholar]
. Ozkan KKU, Boran C, Kilinc M, Garipardiç M, Kurutaş EB, The effect of zinc aspartate pretreatment on isch-emia-reperfusion injury and early changes of blood and tissue antioxidant enzyme activitiesafter unilateral testicular torsion-detorsion J Pediatr Surg 2004 39:91-95. [Google Scholar]
. Johnson FC, The antioxidant vitamins CRC Crit Rev Food Sci Nutr 1979 11:217-309. [Google Scholar]
. Paolicchi A, Pezzini A, Saviozzi M, Localization of a GSH-dependent dehydroascor-bate reductase in rat tissues and subcellular fractions Arch Biochem Biophys 1996 333:489-95. [Google Scholar]
. Verma RJ, Nair A, Ameliorative effect of vitamin E on aflatoxin-induced lipid peroxidation in the testis of mice Asian J Androl 2001 3:217-21. [Google Scholar]
. Yoganathan T, Eskild W, Hansson V, Investigation of detoxification capacity of rat testiculargerm cells and Sertoli cells Free Radic Biol Med 1989 7:355-9. [Google Scholar]
. Takasaki N, Tonami H, Simizu A, Ueno N, Ogita T, Okada S, Semen selenium in male infertility Bull Osaka Med Sch 1987 33(1):87-96. [Google Scholar]
. Keskes Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammani S, Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men Arch Androl 2003 49(2):83-94. [Google Scholar]
. Awad H, Halawa F, Mostafa T, Atta H, Melatonin hormone profile in infertile males Int J Androl 2006 29:409-13. [Google Scholar]
. Liu Z, Lin H, Ye S, Liu QY, Meng Z, Remarkably high activities of testicular cytochrome c in destroy-ing reactive oxygen species and in triggering apoptosis Proc Natl Acad Sci USA 2006 103:8965-70. [Google Scholar]
. Quinn PG, Payne AH, Oxygen mediated damage of microsomal cytochrome P-450 enzymes in cultured leydig cells: Role in steroid genic desensitization J Biol Chem 1984 259:4130-15. [Google Scholar]
. Sheweita SA, Tilmisany AM, Al-Sawaf H, Mechanisms of male infertility: role of antioxidants Curr Drug Metab 2005 6(5):495-501. [Google Scholar]
. Saleh RA, Agarwal A, Oxidative stress and male infertility: from research bench to clinical practice J Androl 2002 23(6):737-52. [Google Scholar]