Year :
2022
| Month :
February
| Volume :
16
| Issue :
2
| Page :
DC30 - DC32
Comparison of Different Phenotypic Methods
Including E-test, Cefoxitin and Oxacillin
Disk Diffusion for Detection of Methicillin
Resistant Staphylococcus aureus
Geeta Gupta, Pradeep Kumar
1. Professor, Department of Microbiology, Santosh Medical College, Ghaziabad, Uttar Pradesh, India.
2. PhD Scholar, Department of Microbiology, Santosh Medical College, Ghaziabad, Uttar Pradesh, India
Correspondence Address :
Pradeep Kumar,
Professor, Department of Microbiology, Santosh Medical College,
Ghaziabad, Uttar Pradesh, India.
E-mail: dr.pradeepmicrologist89@gmail.com
Abstract
Introduction: Methicillin-Resistant Staphylococcus aureus (MRSA) has spread throughout the world as a hospital and community-acquired illness. Although, a variety of strategies have been employed, laboratory identification of MRSA remains a difficulty.
Aim: To examine several phenotypic approaches for accuracy results, with an (Epsilometer) E-test based method serving as the gold standard for MRSA identification.
Materials and Methods: A prospective observational study was conducted in the Microbiology Department of Santosh Medical College, Ghaziabad, Uttar Pradesh, India, from August 2020 to July 2021. Total of 384 isolates S. aureus were identified by using the required samples including pus, swab, blood, wound and urine, etc., which were collected from the Microbiology department and the comparison was done between E-test serving as the gold standard for MRSA identification with Cefoxitin Disk Diffusion (CDD)/Oxacillin Disk Diffusion method (ODD). The diagnostic kit for using E-test in collected samples was purchased from Himedia Laboratries Pvt., Ltd., Mumbai, India (EM0065). The data was calculated by using MS-Excel.
Results: A total of 113 strains were revealed to be MRSA in clinical specimens out of 384 isolated S. aureus according to Cefoxitin (disc diffusion method). The gold standard method was chosen to be the E-test, which had found a high sensitivity of 79.8% and a specificity of 94.2% compared to the cefoxitin/ODD method. Isolates including MRSA were highly susceptible to teicoplanin and linezolid.
Conclusion: The present study concludes that E-test (strip) method is a high sensitivity and highly specific for detecting MRSA in comparison to other disk methods used in this study. Due to less number of sample size and lesser time period more studies are needed to establish this fact.
Keywords
Linezolid, Mueller hinton agar, Oxacillin disc test, Resistant, Teicoplanin
DOI and Others
DOI: 10.7860/JCDR/2022/53064.16018
Date of Submission: Oct 30, 2021
Date of Peer Review: Nov 27, 2021
Date of Acceptance: Jan 12, 2022
Date of Publishing: Feb 01, 2022
AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. NA
PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Nov 01, 2021
• Manual Googling: Dec 31, 2021
• iThenticate Software: Jan 12, 2022 (8%)
ETYMOLOGY: Author Origin
|