Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : May | Volume : 12 | Issue : 5 | Page : ZC10 - ZC14

Estimation of Salivary Tumour Necrosis Factor-a Levels in Post-menopausal Women with Chronic Periodontitis

Pallavi Agrawal, Sheetal Sanikop, Suvarna Patil, Prateek Agrawal, Anil Agrawal, Anil Malleshappa

1. Senior Lecturer, Department of Periodontology, School of Dental Sciences, Sharda University, Greater Noida, Uttar Pradesh, India. 2. Professor, Department of Periodontology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India. 3. Professor, Department of Periodontology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India. 4. Senior Lecturer, Department of Prosthodontics, K.D. Dental College and Hospital, Mathura, Uttar Pradesh, India. 5. Private Practitioner, Department of Periodontology, Dr. Anil Agrawal Dental Specialty Centre, Agra, Uttar Pradesh, India. 6. Professor, Department of Biochemistry, J.N. Medical College, Belgaum, Karnataka, India.

Correspondence Address :
Dr. Pallavi Agrawal,
Senior Lecturer, Department of Periodontology, School of Dental Sciences, Sharda University, Greater Noida-201306, Uttar Pradesh, India.
E-mail: pallavi_07@hotmail.com

Abstract

Introduction: Periodontitis and increased levels of Tumor Necrosis Factor-alpha (TNF-a) are considered as key factors contributing to the bone loss observed in oestrogen deficient post-menopausal women.

Aim: The present study aimed at assessing the levels of salivary TNF-a in pre- and post-menopausal women with chronic periodontitis and evaluating its potential as a biochemical marker of periodontal disease in post menopausal women.

Materials and Methods: A total of 80 female subjects, within the age range of 35-55 years, were divided into 4 groups of 20 subjects each, pre- and post-menopausal women with chronic periodontitis and pre- and post-menopausal periodontally healthy controls. After assessment of clinical periodontal status, unstimulated whole saliva was collected and levels of TNF-a were examined using ELISA. One-way ANOVA, post-hoc Tukey’s test, Student’s unpaired t-test and Karl Pearson’s co-efficient of correlation were used. SPSS version 17.0 and MedCalc version 10.2 were used to perform the data analyses.

Results: The mean of salivary TNF-a levels in pre- and post-menopausal women with chronic periodontitis were 4.25±1.20 pg/mL and 5.13±1.35 pg/mL, respectively, whereas that in pre- and post-menopausal healthy controls were 2.05±1.11 pg/mL and 2.40±1.08 pg/mL, respectively. The levels of salivary TNF-a were significantly higher in the periodontitis groups than the controls and correlated positively with the assessed clinical parameters (p<0.001). Also the levels were significantly higher in post-menopausal women with chronic periodontitis than the pre-menopausal women with chronic periodontitis (p<0.05).

Conclusion: Salivary levels of TNF-a were increased in both menopause and chronic periodontitis. This increased secretion of TNF-a could be implicated as a contributing factor in the progression of periodontal disease in post-menopausal women, suggesting that salivary TNF-a can act as a potential biomarker and a valuable adjunct for screening of periodontal disease in post-menopausal women.

Keywords

Menopause, Periodontitis, Salivary biomarker

How to cite this article :

Pallavi Agrawal, Sheetal Sanikop, Suvarna Patil, Prateek Agrawal, Anil Agrawal, Anil Malleshappa. ESTIMATION OF SALIVARY TUMOUR NECROSIS FACTOR-A LEVELS IN POST-MENOPAUSAL WOMEN WITH CHRONIC PERIODONTITIS. Journal of Clinical and Diagnostic Research [serial online] 2018 May [cited: 2018 May 21 ]; 12:ZC10-ZC14. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=May&volume=12&issue=5&page=ZC10-ZC14&id=11488

DOI and Others

DOI: 10.7860/JCDR/2018/32504.11488

Date of Submission: Sep 12, 2017
Date of Peer Review: Dec 29, 2017
Date of Acceptance: Feb 16, 2018
Date of Publishing: May 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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