Estimation of Salivary Tumour Necrosis Factor-a Levels in Post-menopausal Women with Chronic PeriodontitisCorrespondence Address :
Dr. Pallavi Agrawal,
Senior Lecturer, Department of Periodontology, School of Dental Sciences, Sharda University, Greater Noida-201306, Uttar Pradesh, India.
Introduction: Periodontitis and increased levels of Tumor Necrosis Factor-alpha (TNF-a) are considered as key factors contributing to the bone loss observed in oestrogen deficient post-menopausal women.
Aim: The present study aimed at assessing the levels of salivary TNF-a in pre- and post-menopausal women with chronic periodontitis and evaluating its potential as a biochemical marker of periodontal disease in post menopausal women.
Materials and Methods: A total of 80 female subjects, within the age range of 35-55 years, were divided into 4 groups of 20 subjects each, pre- and post-menopausal women with chronic periodontitis and pre- and post-menopausal periodontally healthy controls. After assessment of clinical periodontal status, unstimulated whole saliva was collected and levels of TNF-a were examined using ELISA. One-way ANOVA, post-hoc Tukey’s test, Student’s unpaired t-test and Karl Pearson’s co-efficient of correlation were used. SPSS version 17.0 and MedCalc version 10.2 were used to perform the data analyses.
Results: The mean of salivary TNF-a levels in pre- and post-menopausal women with chronic periodontitis were 4.25±1.20 pg/mL and 5.13±1.35 pg/mL, respectively, whereas that in pre- and post-menopausal healthy controls were 2.05±1.11 pg/mL and 2.40±1.08 pg/mL, respectively. The levels of salivary TNF-a were significantly higher in the periodontitis groups than the controls and correlated positively with the assessed clinical parameters (p<0.001). Also the levels were significantly higher in post-menopausal women with chronic periodontitis than the pre-menopausal women with chronic periodontitis (p<0.05).
Conclusion: Salivary levels of TNF-a were increased in both menopause and chronic periodontitis. This increased secretion of TNF-a could be implicated as a contributing factor in the progression of periodontal disease in post-menopausal women, suggesting that salivary TNF-a can act as a potential biomarker and a valuable adjunct for screening of periodontal disease in post-menopausal women.
Menopause, Periodontitis, Salivary biomarker
Pallavi Agrawal, Sheetal Sanikop, Suvarna Patil, Prateek Agrawal, Anil Agrawal, Anil Malleshappa. ESTIMATION OF SALIVARY TUMOUR NECROSIS FACTOR-A LEVELS IN POST-MENOPAUSAL WOMEN WITH CHRONIC PERIODONTITIS. Journal of Clinical and Diagnostic Research [serial online] 2018 May [cited: 2018 May 21 ]; 12:ZC10-ZC14. Available from
Date of Submission: Sep 12, 2017
Date of Peer Review: Dec 29, 2017
Date of Acceptance: Feb 16, 2018
Date of Publishing: May 01, 2018
FINANCIAL OR OTHER COMPETING INTERESTS: None.
- Emerging Sources Citation Index (Web of Science, thomsonreuters)
- Index Copernicus ICV 2016: 132.37
- Academic Search Complete Database
- Directory of Open Access Journals (DOAJ)
- Embase & EMbiology
- Google Scholar
- HINARI Access to Research in Health Programme
- Indian Science Abstracts (ISA)
- Journal seek Database
- Popline (reproductive health literature)