Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : June | Volume : 12 | Issue : 6 | Page : DC31 - DC36

Assessment of Knack of Clinical Staphylococcus Aureus Isolates for the Biofilm Formation and Presence of icaABCD Family Genes

Alka Hasani, Leila Dehghani, Mohammad Ahangarzadeh Rezaee, Akbar Hasani, Mohammad Asghari Jafarabadi, Naser Alizadeh, Nasim Asadi, Hossein Samadi Kafil

1. Infectious and Tropical Diseases Research Center, Department of Medical Microbiology, Tabriz University of Medical Sciences, Tabriz, Az-Sharghi, Iran. 2. Infectious and Tropical Diseases Research Center, Department of Medical Microbiology, Tabriz University of Medical Sciences, Tabriz, Az-Sharghi, Iran. 3. Infectious and Tropical Diseases Research Center, Department of Medical Microbiology, Tabriz University of Medical Sciences, Tabriz, Az-Sharghi, Iran. 4. Infectious and Tropical Diseases Research Center, Department of Medical Microbiology, Tabriz University of Medical Sciences, Tabriz, Az-Sharghi, Iran. 5. Infectious and Tropical Diseases Research Center, Department of Medical Microbiology, Tabriz University of Medical Sciences, Tabriz, Az-Sharghi, Iran. 6. Infectious and Tropical Diseases Research Center, Department of Medical Microbiology, Tabriz University of Medical Sciences, Tabriz, Az-Sharghi, Iran.

Correspondence Address :
Dr. Hossein Samadi Kafil,
Golgasht Ave faculty of Medicine, Department of Medical Microbiology, Tabriz, Az-Sharghi, Iran.
E-mail: kafilhs@tbzmed.ac.ir

Abstract

Introduction: Superbug known as Staphylococcus aureus possess a tendency to form biofilm, which has a significant role in causing infection and abating host defense response. Amongst many mechanisms, biofilm formation depends on the icaABCD operon involved in the synthesis of a polysaccharide intercellular adhesion.

Aim: To investigate biofilm forming ability of S. aureus isolates by phenotypic and genotypic methods.

Materials and Methods: Of the 97 S. aureus clinical isolates collected, the quantitative biofilm formation was determined by microtiter plates. All S. aureus isolates were examined for detection of the icaABCD genes and mecA gene by using PCR method. Statistical analysis was performed with SPSS program version 17.0.

Results: Among 97 S. aureus isolates from blood, wound, skin, surgery, internal, burn and infectious wards, urine and body fluids specimens, five isolates appeared as strong biofilm producer, while 28 displayed moderate biofilm formation, and 55 showed weak biofilm formation. Nine isolates did not reveal biofilm production on microtiter plates. The frequency of icaA, icaB, icaC and icaD genes in S. aureus isolates was 81 (83.5%), 71 (73.2%), 51 (52.5%) and 97 (100%), respectively. There was no relation between presence of icaABCD genes and biofilm formation (p=0.74).

Conclusion: The presence of biofilm genes may not coincide with the ability to produce biofilm or vice versa. At the results, S. aureus clinical isolates possess different capacity to produce biofilm and adhesion. Methicillin resistance and susceptible isolates may not differ in their capacity to form biofilm.

Keywords

Adhesion, Antibiotic susceptibility pattern, Methicillin resistance, Pathogenesis, Polysaccharide

How to cite this article :

Alka Hasani, Leila Dehghani, Mohammad Ahangarzadeh Rezaee, Akbar Hasani, Mohammad Asghari Jafarabadi, Naser Alizadeh, et al.. ASSESSMENT OF KNACK OF CLINICAL STAPHYLOCOCCUS AUREUS ISOLATES FOR THE BIOFILM FORMATION AND PRESENCE OF ICAABCD FAMILY GENES. Journal of Clinical and Diagnostic Research [serial online] 2018 June [cited: 2018 Jun 23 ]; 12:DC31-DC36. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=June&volume=12&issue=6&page=DC31-DC36&id=11636

DOI and Others

DOI: 10.7860/JCDR/2018/34140.11636

Date of Submission: Oct 06, 2017
Date of Peer Review: Nov 08, 2017
Date of Acceptance: Mar 20, 2018
Date of Publishing: Jun 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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