Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

Users Online : 12217

Original article / research
Year : 2018 | Month : June | Volume : 12 | Issue : 6 | Page : BC17 - BC20

Markers of Oxidative Stress, Inflammation and Endothelial Dysfunction in ESRD Patients with Hepatitis-C Virus Positive on Maintenance Haemodialysis

Krishna Kishore Chennu, Niimmanapalli Harini Devi, PVLN Srinivasa Rao, AY Lakshmi, Vishnubotla Siva Kumar

1. Consultant, Department of Nephrology, Narayana hospitals, Bangalore, Karnataka, India. 2. Assistant professor, Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India. 3. Senior Professor and Head, Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India. 4. Professor and Head, Department of Radio-Diagnosis, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India. 5. Senior Professor, Department of Nephrology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India.

Correspondence Address :
Dr. P.V.L.N. Srinivasa Rao,
Senior Professor and Head, Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences,
Tirupati-517507, Andhra Pradesh, India.
E-mail: seenupvln@yahoo.com

Abstract

Introduction: Hepatitis C Virus (HCV) infection is the significant cause of morbidity and mortality in Haemodialysis (HD) patients and kidney transplant recipients. HD technique may contribute to maintaining the inflammatory state in many HD patients. Oxidative stress, inflammation and endothelial dysfunction are common in patients with End-Stage Renal Disease (ESRD).

Aim: To assess the possible relationships between oxidative stress, inflammatory and endothelial dysfunction markers with HCV positive status on maintenance HD.

Materials and Methods: The study enrolled 22 patients with ESRD, seropositive for HCV; 22 patients with ESRD, seronegative for HCV on maintenance HD and 19 healthy subjects as controls. In the present study, high sensitivity C-reactive protein (hs-CRP) was estimated by immunoturbidimetry method, Homocysteine (Hcy) was estimated by enzymatic recycling method, Malondialdehyde (MDA), Ferric Reducing Ability of Plasma (FRAP) and Nitric oxide (NO) were estimated by spectrophotometric method and Carotid Intima Media Thickness (CIMT) was estimated by Carotid Ultrasonography.

Results: There is statistically significant increase in serum MDA, Hcy, hs-CRP, CIMT levels and statistically significant decrease in serum NO and FRAP levels in HCV positive and HCV negative groups when compared to controls. Correlation analysis of CIMT with other markers in the study showed statistically significant negative correlations among CIMT with FRAP and NO and statistically significant positive correlations among CIMT with hs-CRP and Hcy but no association with MDA.

Conclusion: Oxidative stress, inflammation, and endothelial dysfunction might be associated with HCV infection in ESRD patients on maintenance HD.

Keywords

Carotid intima media thickness, Malondialdehyde, Nitric oxide, Seronegative, Seropositive

How to cite this article :

Krishna Kishore Chennu, Niimmanapalli Harini Devi, PVLN Srinivasa Rao, AY Lakshmi, Vishnubotla Siva Kumar. MARKERS OF OXIDATIVE STRESS, INFLAMMATION AND ENDOTHELIAL DYSFUNCTION IN ESRD PATIENTS WITH HEPATITIS-C VIRUS POSITIVE ON MAINTENANCE HAEMODIALYSIS. Journal of Clinical and Diagnostic Research [serial online] 2018 June [cited: 2018 Jun 18 ]; 12:BC17-BC20. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=June&volume=12&issue=6&page=BC17-BC20&id=11689

DOI and Others

DOI: 10.7860/JCDR/2018/31614.11689

Date of Submission: Jul 15, 2017
Date of Peer Review: Aug 29, 2017
Date of Acceptance: Nov 22, 2017
Date of Publishing: Jun 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: None.

JCDR is now Monthly and more widely Indexed .
  • Emerging Sources Citation Index (Web of Science, thomsonreuters)
  • Index Copernicus ICV 2016: 132.37
  • SCOPUS
  • Academic Search Complete Database
  • Directory of Open Access Journals (DOAJ)
  • EBSCOhost
  • Embase & EMbiology
  • Google Scholar
  • HINARI Access to Research in Health Programme
  • Indian Science Abstracts (ISA)
  • Journal seek Database
  • Google
  • Popline (reproductive health literature)
  • www.omnimedicalsearch.com