Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : July | Volume : 12 | Issue : 7 | Page : BC22 - BC25

Evaluating the Feasibility of Nested PCR as a Screening Tool to Detect HPV Infection in Saliva of Oral Squamous Cell Carcinoma Subjects

Pushkal Sinduvadi Ramesh, Devananda Devegowda, Pundalik Rama Naik, Prathibha, Parveen Doddamani, Suma M Nataraj

1. Assistant Professor, Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India. 2. Director Professor, Department of Microbiology, Govind Ballabh Pant Institute of Post Graduate Medical Education and Research, New Delhi, India. 3. Director Professor and Head, Department of Microbiology, Govind Ballabh Pant Institute of Post Graduate Medical Education and Research, New Delhi, India. 4. Professor, Department of Microbiology, Govind Ballabh Pant Institute of Post Graduate Medical EdUcation and Research, New Delhi, India. 5. Director Professor and Head, Department of Microbiology, Govind Ballabh Pant Institute of Post Graduate Medical Education and Research, New Delhi, India

Correspondence Address :
Dr. Mohit Bhatia,
Assistant Professor, Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
E-mail: mohit.micro@aiimsrishikesh.edu.in

Abstract

Clinical Microbiologists often come across strange structures during microscopic examination of clinical specimens which are often confused with and misreported as micro-organisms. We studied series of cases in a super speciality hospital from January 2015 to December 2016 with the aim of enumerating artefacts and mimickers that may simulate infectious agents. Microscopic examination of various clinical specimens like pus, tissue, sputum and blood (for peripheral smear examination) received by the department of Microbiology during this period was carried out as per requisition received from clinicians. Photographs of several microscopic artefacts were obtained using a high definition camera. For comparative purpose, photographs of common pathogenic micro-organisms closely resembling these structures were also obtained. Various microscopic artefacts closely resembling pathogenic micro-organisms like fungal hyphae, microfilariae, malaria parasites, acid fast bacilli, budding yeast cells and hooklets of Echinococcus granulosus were observed during the course of this study and enumerated. Owing to paucity of available literature, it is absolutely essential to highlight the issue of misreporting of infectious diseases during microscopic examination of clinical specimens.

Keywords

HPV genotyping, PGMY09/PGMY11 primers, GP5+/GP6+ primers, Non-invasive

How to cite this article :

Pushkal Sinduvadi Ramesh, Devananda Devegowda, Pundalik Rama Naik, Prathibha, Parveen Doddamani, Suma M Nataraj. EVALUATING THE FEASIBILITY OF NESTED PCR AS A SCREENING TOOL TO DETECT HPV INFECTION IN SALIVA OF ORAL SQUAMOUS CELL CARCINOMA SUBJECTS. Journal of Clinical and Diagnostic Research [serial online] 2018 July [cited: 2018 Jul 17 ]; 12:BC22-BC25. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=July&volume=12&issue=7&page=BC22-BC25&id=11806

DOI and Others

DOI: 10.7860/JCDR/2018/34880.11806

Date of Submission: Nov 21, 2017
Date of Peer Review: Feb 13, 2018
Date of Acceptance: May 09, 2018
Date of Publishing: July 01, 2018

Financial OR OTHER COMPETING INTERESTS: None.

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