Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : August | Volume : 12 | Issue : 8 | Page : DC21 - DC23

Effect of Hydroalcoholic Extract of Arnebia Euchroma on the Treatment of Cutaneous Leishmaniasis

Roham Borazjani, Shiva Aminnia, Mohammad Rastegarian, Mahnaz Hosseini, Zahra Ghanbarinasab, Soheil Ashkani-Esfahani, Bahador Sarkari

1. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. 5. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. 6. Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran. 7. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Correspondence Address :
Dr. Bahador Sarkari,
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
E-mail: sarkarib@sums.ac.ir

Abstract

Introduction: The growing resistance of the Leishmania parasites to the available anti-leishmanial drugs has been reported from different areas of the world, including Iran.

Aim: To assess the effect of hydroalcoholic extract of Arnebia euchroma, for treatment of cutaneous leishmaniasis induced by Leishmania major in BALB/c mice.

Materials and Methods: Arnebia euchroma extract was provided by medicinal herb garden in Yasuj, in the south of Iran. To facilitate the application of the extract, 5% gel with Arnebia extract and Carboxymethylcellulose (CMC) was prepared. Mice infection was done by subcutaneous inoculation of amastigotes form of Leishmania major. Lesions were developed after about three weeks of inoculating. Mice were divided into four groups, (six mice in each group), where the first group was treated with 5% of the extract and the second group with 5% gel of Arnebia euchroma. The third group was treated by meglumine antimoniate. The negative control group received only normal saline. Treatments was started from the day open lesion were observed and carried out every third day for 21 days. The size of lesion on tail base were measured weekly.

Results: Mean sizes of lesions in mice treated with the extract or topical gel were lower than the controls. However, the differences were not statistically significant (p>0.05).

Conclusion: Findings of the study showed that Arnebia euchroma extract in doses which have been used in this study (5%) and in comparison with meglumine antimoniate had no considerable effect in treatment or control of cutaneous leishmaniasis.

Keywords

Carboxymethylcellulose (CMC), Meglumine antimoniate, Subcutaneous inoculation

How to cite this article :

Roham Borazjani, Shiva Aminnia, Mohammad Rastegarian, Mahnaz Hosseini, Zahra Ghanbarinasab, Soheil Ashkani-Esfahani, et al.. EFFECT OF HYDROALCOHOLIC EXTRACT OF ARNEBIA EUCHROMA ON THE TREATMENT OF CUTANEOUS LEISHMANIASIS. Journal of Clinical and Diagnostic Research [serial online] 2018 August [cited: 2018 Jul 18 ]; 12:DC21-DC23. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=August&volume=12&issue=8&page=DC21-DC23&id=11906

DOI and Others

DOI: 10.7860/JCDR/2018/35856.11906

Date of Submission: Feb 02, 2018
Date of Peer Review: Apr 04, 2018
Date of Acceptance: Jun 04, 2018
Date of Publishing: Aug 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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