Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : August | Volume : 12 | Issue : 8 | Page : DC10 - DC13

Validation of a Rapid Stool Antigen Test for the Diagnosis of Helicobacter pylori Infection in Dyspeptic Patients-A Study from Central Kerala

Suryakala Ravi Nair, Prithi Nair Kannambra, Saji K Sebastian

1. Assistant Professor, Department of Microbiology, Government Medical College, Thrissur, Kerala, India. 2. Professor and Head, Department of Microbiology, Government Medical College, Thrissur, Kerala, India. 3. Associate Professor, Department of Gastroenterology, Government Medical College, Thrissur, Kerala, India.

Correspondence Address :
Dr. Suryakala Ravi Nair,
Saaya, Vattekkattu House, Ollukkara. P.O. Kalathode, Thrissur-680655, Kerala, India.
E-mail: suryaofsunil@gmail.com

Abstract

Introduction: Accurate diagnosis of Helicobacter pylori infection in dyspeptic patients is highly essential to institute eradication therapy and to prevent complications. The introduction of ‘test and treat strategy’ using validated rapid stool antigen tests can significantly reduce the burden of H.pylori infection in developing countries.

Aim: To validate a rapid monoclonal immunochromatographic stool antigen test, Epituub® fecal H.pylori antigen test kit for the diagnosis of Helicobacter pylori infection among dyspeptic patients.

Materials and Methods: Stool samples were collected for Epituub® fecal H.pylori antigen test from randomly selected patients undergoing upper gastrointestinal endoscopy for the evaluation of dyspepsia. Gastric biopsy samples were also collected for urease test and histopathology. The diagnostic criteria for H.pylori infection was defined as a positive test result for both rapid urease test and histopathology examination. All other combination of the results was considered as negative. The test performance was assessed by determining sensitivity, specificity, positive predictive value and negative predictive value with reference to histopathology examination and rapid urease test.

Results: Based on the reference criteria, 31% (35/113) patients were diagnosed as H. pylori infected and 41% (46/113) were rapid stool antigen test positive. The sensitivity, specificity, positive predictive value and negative predictive value of the Epituub® fecal H.pylori antigen test kit were 88.5%, 80.76%, 67.3% and 94.02% respectively.

Conclusion: Epituub® fecal H.pylori antigen test can be used as a valid alternative to invasive tests for the diagnosis of H.pylori infection. It is also relatively cheap, fast and easy to perform.

Keywords

Gastrointestinal endoscopy, Histopathology, Sensitivity, Specificity

How to cite this article :

Suryakala Ravi Nair, Prithi Nair Kannambra, Saji K Sebastian. VALIDATION OF A RAPID STOOL ANTIGEN TEST FOR THE DIAGNOSIS OF HELICOBACTER PYLORI INFECTION IN DYSPEPTIC PATIENTS-A STUDY FROM CENTRAL KERALA. Journal of Clinical and Diagnostic Research [serial online] 2018 August [cited: 2018 Jul 23 ]; 12:DC10-DC13. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=August&volume=12&issue=8&page=DC10-DC13&id=11871

DOI and Others

DOI: 10.7860/JCDR/2018/36487.11871

Date of Submission: Apr 06, 2018
Date of Peer Review: May 10, 2018
Date of Acceptance: Jun 28, 2018
Date of Publishing: Aug 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: As declared above.

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