Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : August | Volume : 12 | Issue : 8 | Page : BC12 - BC15

Case Control Study of Oxidised LDL & Serum Paraoxanase Activity in Ischaemic Stroke Patients Attending Tertiary Care Centre in South Kerala

Poornima Bijumon, Geetha Ananthashenoyi, Thomas Iype

1. Assistant Professor, Department of Biochemistry, Malabar Medical College Hospital and Research Centre, Modakkallur, Kerala, India. 2. Professor and Head, Department of Biochemistry, Government Medical College, Kottayam, Kerala, India. 3. Professor and Head, Department of Neurology, Government Medical College, Trivandrum, Kerala, India.

Correspondence Address :
Dr. Geetha Ananthashenoyi,
Professor and Head, Department of Biochemistry, Government Medical College, Kottayam-686008, Kerala, India.
E-mail: drgeethakamath@gmail.com

Abstract

Introduction: Oxidative stress by producing lipid peroxidation products like Oxidised Low Density Lipoprotein (LDL) acts as independent risk factor in ischaemic stroke. When lipid peroxidation overwhelms antioxidative defense mechanism; it results in enhanced formation of oxidized LDL which promotes atherosclerosis. Human serum Paraoxanase (PON 1) which is an ester hydrolase synthesized in the liver prevents the oxidative modification of LDL.

Aim: To investigate the level of oxidized LDL as oxidative stress marker and paraoxonase activity as an antioxidant enzyme in ischaemic stroke patients and also the relation between Oxidized-Low Density Lipoprotein (Ox-LDL) level and Computed Tomography (CT) findings in ischaemic stroke patients.

Materials and Methods: The subjects in this study comprised of 40 ischaemic stroke patients and 40 age and gender matched controls. Fasting plasma glucose, serum paraoxanase activity, plasma oxidised LDL level, fasting lipid profile were determined. Data was analysed using Statistical Package for Social Sciences (SPSS) version 16.0.

Results: Ischaemic stroke patients had significantly lower paraoxanase activity (<53 kU/L), elevated levels of oxidized LDL (>2.26 g/ml), total cholesterol (>200 mg%) and fasting plasma glucose levels (>126 mg%).

Conclusion: Oxidised LDL level >2.26 g/ml, paraoxanase activity <53 kU/L and FBS >126 mg% were independent risk factors for stroke. Significantly higher levels of oxidized LDL were seen in patients with infarct in anterior circulation. This study implies that raised oxidized LDL level and low serum paraoxanase activity are independent risk factors of ischaemic stroke in patients with no other risk factors.

Keywords

Antioxidant, Hyperglycaemia, Oxidative stress

How to cite this article :

Poornima Bijumon, Geetha Ananthashenoyi, Thomas Iype. CASE CONTROL STUDY OF OXIDISED LDL & SERUM PARAOXANASE ACTIVITY IN ISCHAEMIC STROKE PATIENTS ATTENDING TERTIARY CARE CENTRE IN SOUTH KERALA. Journal of Clinical and Diagnostic Research [serial online] 2018 August [cited: 2018 Jul 23 ]; 12:BC12-BC15. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=August&volume=12&issue=8&page=BC12-BC15&id=11910

DOI and Others

DOI: 10.7860/JCDR/2018/36402.11910

Date of Submission: Mar 13, 2018
Date of Peer Review: May 26, 2018
Date of Acceptance: Jun 08, 2018
Date of Publishing: Aug 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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