Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : November | Volume : 11 | Issue : 11 | Page : ZC26 - ZC30

Comparative Evaluation of the Antibacterial Efficacy of two different Formulations of Calcium hydroxide as Intracanal Medicaments against Enterococcus faecalis: An In vitro study

Priya Sapra, Anand C Patil, Kishore Bhat, Amritpal S Kullar

1. Ex-postgraduate Student, Department of Conservative Dentistry and Endodontics, KLE VK Institute of Dental Sciences, Belagavi, Karnataka, India. 2. Professor and Head, Department of Conservative Dentistry and Endodontics, KLE VK Institute of Dental Sciences, Belagavi, Karnataka, India. 3. Consultant, Department of Microbiology, Dr. Prabhakar Kore Hospital and Research Centre, KLE University, Belagavi, Karnataka, India. 4. Postgraduate Student, Department of Oral Surgery, NYU College of Dentistry, New York, USA.

Correspondence Address :
Dr. Priya Sapra,
Ex-postgraduate Student, Department of Conservative Dentistry and Endodontics,
D-105, Vikaspuri, New Delhi-110018, India.
E-mail: saprapriya87@gmail.com

Abstract

Introduction: Complete disinfection of the root canal system is necessary to ensure the success of the root canal treatment. Calcium hydroxide alone has shown to be ineffective against persistent intracanal infections. Studies have shown that addition of newer antimicrobial agents to calcium hydroxide has a synergistic effect on its antibacterial properties.

Aim: To evaluate the disinfection of dentinal tubules using calcium hydroxide with imipenem and calcium hydroxide with octenidine Hydrochloride (OCT) as compared to calcium hydroxide alone against Enterococcus faecalis (E.faecalis).

Materials and Methods: The antimicrobial efficacy of the medicaments against E. faecalis was assessed in vitro, using a dentinal tubule model at a depth of 400 µm in extracted single rooted permanent human mandibular premolar teeth. The dentin blocks (n=125) were contaminated with E. faecalis for 21 days. At the end of 21 days, five blocks were selected randomly and depth of penetration of microorganisms was checked using Scanning Electron Microscopy (SEM). The blocks (n=120) were irrigated using saline and randomly divided in three groups (n=40) depending on the medicament placed in the canals Group I (control)- Calcium Hydroxide {Ca(OH)2} +Saline, Group II-Ca(OH)2+imipenem, Group III- Ca(OH)2+OCT. Twenty blocks were taken from each group on 2nd and 7th day respectively. Medicament was removed. Dentin debris were harvested at a depth of 400 µm and at the end of 24 hours, optical density was evaluated using ELISA reader at 620 nm. Statistical analysis was carried out using Analysis of variance (ANOVA) and Tukey’s multiple post hoc test.

Results: All the three experimental groups of medicaments exhibited antibacterial efficacy against E. faecalis on day 2 and day 7. However, when mean optical density was compared, the antibacterial efficacy of Group II on 7th day was significantly higher than Group I on 2nd day and 7th day and Group III on 2nd day.

Conclusion: All the tested medicaments in this study exhibited antibacterial activity against E. faecalis on day 2 and day 7 but the most potent medicament against E. faecalis was the combination of Ca(OH)2 mixed with imipenem for 7 days.

Keywords

Beta-lactam, Dentin, Imipenem, Microbiota

How to cite this article :

Priya Sapra, Anand C Patil, Kishore Bhat, Amritpal S Kullar. COMPARATIVE EVALUATION OF THE ANTIBACTERIAL EFFICACY OF TWO DIFFERENT FORMULATIONS OF CALCIUM HYDROXIDE AS INTRACANAL MEDICAMENTS AGAINST ENTEROCOCCUS FAECALIS: AN IN VITRO STUDY. Journal of Clinical and Diagnostic Research [serial online] 2017 November [cited: 2018 Jan 23 ]; 11:ZC26-ZC30. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=November&volume=11&issue=11&page=ZC26-ZC30&id=10885

DOI and Others

DOI: 10.7860/JCDR/2017/27270.10885

Date of Submission: Mar 16, 2017
Date of Peer Review: May 22, 2017
Date of Acceptance: Aug 26, 2017
Date of Publishing: Nov 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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