Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

Users Online : 52914

Original article / research
Year : 2017 | Month : November | Volume : 11 | Issue : 11 | Page : ZC18 - ZC21

Histological Evaluation of Epithelial Thickness, Microvasculature and Number of Mast Cells in the Gingiva of Smokers and Non-smokers with Chronic Periodontitis

Jose Paul, Johnson Prakash D’Lima, Senny Thomas, Shermin Karim, Priya Thomas,Bindhu Pushparajan Ramakrishnan, Binitta Paul, Rifat Salma

1. Professor, Department of Periodontics, Annoor Dental College and Hospital, Muvattupuzha, Kerala, India. 2. Professor, Department of Periodontics, Annoor Dental College and Hospital, Muvattupuzha, Kerala, India. 3. Professor, Department of Periodontics, Annoor Dental College and Hospital, Muvattupuzha, Kerala, India. 4. Reader, Department of Periodontics, Annoor Dental College and Hospital, Muvattupuzha, Kerala, India. 5. Reader, Department of Oral Pathology and Microbiology, Annoor Dental College and Hospital, Muvattupuzha, Kerala, India. 6. Professor, Department of Oral Pathology and Microbiology, Annoor Dental College and Hospital, Muvattupuzha, Kerala, India. 7. Senior Lecturer, Department of Periodontics, Annoor Dental College and Hospital, Muvattupuzha, Kerala, India. 8. Postgraduate Student, Department of Periodontics, Annoor Dental College and Hospital, Muvattupuzha, Kerala, India.

Correspondence Address :
Dr. Johnson Prakash D'lima,
Vailankanni Villa, Church Road, Kundapura, Udupi District, Karnataka-576201, India.
E-mail: johnsonperio@gmail.com

Abstract

Introduction: Smoking influences the clinical parameters and disease progression in many ways. Increase in the gingival epithelial thickness and alteration in the microvasculature can be associated with decreased clinical sings of inflammation and bleeding on probing.

Aim: To histologically compare the epithelial thickness, vascular density, lumen area and the number of mast cells in the gingiva of smokers and non-smokers with chronic periodontitis.

Materials and Methods: Gingival biopsies were obtained from 30 chronic periodontitis patients (15 smokers and 15 non-smokers) undergoing due to poor periodontal prognosis. Two sections were obtained and were stained with Haematoxylin & Eosin (H&E) and toluidine blue. The epithelial thickness, vascular density, lumen area of blood vessels and number of mast cells were assessed using histomorphometric image analysis.

Results: Results of this study showed that the mean epithelial thickness among smokers was significantly increased when compared to non-smokers. The vascular density (p-value 0.790), area of lumen (p-value 0.790) of blood vessels were more in smokers than in non-smokers but did not show any significant difference. The number of mast cells were increased in smokers with significant difference when compared with non-smokers.

Conclusion: Based on the present histomorphometric study, it can be concluded that smokers have increased epithelial thickness which could be attributed to the reduced clinical signs of inflammation, but vascular density and lumen area were comparable to non-smokers. The mast cell density was higher in smokers which could be attributed to the increased disease severity.

Keywords

Gingival epithelium, Gingival microvasculature, ImageJ analysis, Periodontal disease

How to cite this article :

Jose Paul, Johnson Prakash D’Lima, Senny Thomas, Shermin Karim, Priya Thomas, Bindhu Pushparajan Ramakrishnan, et al.. HISTOLOGICAL EVALUATION OF EPITHELIAL THICKNESS, MICROVASCULATURE AND NUMBER OF MAST CELLS IN THE GINGIVA OF SMOKERS AND NON-SMOKERS WITH CHRONIC PERIODONTITIS. Journal of Clinical and Diagnostic Research [serial online] 2017 November [cited: 2018 Jan 21 ]; 11:ZC18-ZC21. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=November&volume=11&issue=11&page=ZC18-ZC21&id=10886

DOI and Others

DOI: 10.7860/JCDR/2017/29112.10886

Date of Submission: May 22, 2017
Date of Peer Review: Jun 22, 2017
Date of Acceptance: Oct 28, 2017
Date of Publishing: Nov 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

JCDR is now Monthly and more widely Indexed .
  • Emerging Sources Citation Index (Web of Science, thomsonreuters)
  • Index Copernicus ICV 2016: 132.37
  • SCOPUS
  • Academic Search Complete Database
  • Directory of Open Access Journals (DOAJ)
  • EBSCOhost
  • Embase & EMbiology
  • Google Scholar
  • HINARI Access to Research in Health Programme
  • Indian Science Abstracts (ISA)
  • Journal seek Database
  • Google
  • Popline (reproductive health literature)
  • www.omnimedicalsearch.com