Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : November | Volume : 11 | Issue : 11 | Page : ZC12 - ZC17

Effect of Punica granatum Extract Gel on Gingival Crevicular Fluid Levels of Interleukin-1, Interleukin-8 and CCL28 Levels: Randomised Controlled Clinical Trial

Jayant Prakash, Vipin Bhatnagar, Sonia Nath, Shaju Pulikkotil, Virendra Kumar Prajapati

1. Senior Lecturer, Department of Prosthodontics, Vananchal Dental College, Ranchi, Jharkhand, India. 2. Professor, Department of Prosthodontics, Saraswati Dental College, Lucknow, Uttar Pradesh, India. 3. Senior Resident, Department of Dentistry, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India. 4. Senior Lecturer, Department of Restorative Dentistry, International Medical University, Kuala Lumpur, Malaysia. 5. Head, Department of Dentistry, Rajendra Institute of Medical Sciences, Ranchi, Jharkhand, India.

Correspondence Address :
Dr. Sonia Nath,
Senior Resident, Department of Dentistry, Rajendra Institute of Medical Sciences, Ranchi-834009, Jharkhand, India.
E-mail: sonianath_12@yahoo.co.in

Abstract

Introduction: Pomegranate (Punica granatum) fruit extract has anti-inflammatory, anti-bacterial, anti-oxidant and immune-modulatory features. Due to increased bacterial resistance to synthetic agents Pomegranate Extract Gel (PEG) could become a natural remedy for the treatment of periodontal disease.

Aim: To compare topically applied PEG, Chlorhexidine (CHX) gel, Ornidazole-Chlorhexidine (ORN-CHX) gel and Placebo Gel (PG) on clinical parameters and on Gingival Crevicular Fluid (GCF) levels of interleukin-1 (IL-1) and IL-8 and Chemokine (C-C motif) ligand 28 (CCL28) levels in a two week human experimental gingivitis model.

Materials and Methods: Eighty systemically healthy subjects were randomly assigned to one of the four groups; Group I: PEG, Group II: CHX gel, Group III: ORN-CHX gel, and Group IV: PG. On the selected quadrant the subjects refrained from routine hygiene procedure and solely used the topical gel. The primary outcome measured were IL-1, IL-8 and CCL28 levels from GCF at baseline and 14 days. Gingival Index (GI), Plaque Index (PI), Bleeding On Probing (BOP) and Probing Depth (PD) were secondarily assessed at baseline, 14 and 60 days. Paired t-test and one-way ANOVA followed by post-hoc Tukeys test was used for intra and intergroup comparisons with p-value of 0.05 as significant.

Results: The PEG had an inhibitory effect on pro-inflammatory cytokines and chemokine. PEG showed significantly less increase in IL-1, IL-8 (p=0.003, 0.002) while CCL28 levels showed no difference from baseline (p=0.15) when compared to other three groups (p<0.001). The BOP and GI among subjects using PEG showed less increase (p=0.01, 0.05) compared to other three gels (p<0.001) after 14 days. PEG and CHX-ORD gel were similar (p=0.96) in exhibiting lowered PI.

Conclusion: Pomegranate extract gel was effective in reducing the inflammatory markers and can be used as an adjunct to mechanical therapy for the treatment of gingivitis.

Keywords

Chlorhexidine, Cytokine, Gingivitis, Oral health, Oral hygiene, Ornidazole

How to cite this article :

Jayant Prakash, Vipin Bhatnagar, Sonia Nath, Shaju Pulikkotil, Virendra Kumar Prajapati. EFFECT OF PUNICA GRANATUM EXTRACT GEL ON GINGIVAL CREVICULAR FLUID LEVELS OF INTERLEUKIN-1, INTERLEUKIN-8 AND CCL28 LEVELS: RANDOMISED CONTROLLED CLINICAL TRIAL. Journal of Clinical and Diagnostic Research [serial online] 2017 November [cited: 2018 Jan 23 ]; 11:ZC12-ZC17. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=November&volume=11&issue=11&page=ZC12-ZC17&id=10845

DOI and Others

DOI: 10.7860/JCDR/2017/31035.10845

Financial OR OTHER COMPETING INTERESTS: None.

Date of Submission: Jun 21, 2017
Date of Peer Review: Aug 26, 2017
Date of Acceptance: Oct 29, 2017
Date of Publishing: Nov 01, 2017

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