Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

Users Online : 9096

Original article / research
Year : 2017 | Month : November | Volume : 11 | Issue : 11 | Page : QC01 - QC04

Evaluation of Semen Oxidative Stress and Sperm DNA Damage in Cases of Unexplained Foetal Congenital Anomalies- A Pilot Study

Japleen Kaur, Dipika Deka, Rima Dada, Perumal Vanamail, Vatsla Dadhwal, Neena Malhotra, Madhulika Kabra

1. Senior Resident, Department of Obstetrics and Gynaecology, Postgraduate Institute of Medical Education and Research, Chandigarh, India. 2. Professor, Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India. 3. Professor, Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India. 4. Assistant Professor, Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India. 5. Professor, Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India. 6. Professor, Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India. 7. Professor, Department of Paediatrics, All India Institute of Medical Sciences, New Delhi, India.

Correspondence Address :
Dr. Japleen Kaur,
Senior Resident, Department of Obstetrics and Gynaecology, 3rd Floor F Block, Nehru Hospital, PGIMER, Chandigarh-160012, India.
E-mail: japleen18@gmail.com

Abstract

Introduction: Birth defects lead to significant neonatal morbidity and mortality. Although maternal factors have been widely studied, paternal factors have not received much attention. Inherent integrity of the sperm DNA is vital to normal embryo development, as it contributes one half of the genetic material to the embryo. One of the mechanisms of sperm DNA damage is oxidative stress.

Aim: This pilot study was conducted to assess the role of sperm factors in unexplained fetal congenital anomalies.

Materials and Methods: 25 couples with antenatally detected fetal anomalies were recruited at a tertiary level hospital. Semen samples from the male partners were evaluated for semen Reactive Oxidative Species (ROS) and sperm DNA Fragmentation Index (DFI) using Luminol-chemiluminescence method and Sperm Chromatin Structure Assay, respectively. Median values were compared for Total Sperm Count and Semen ROS, using non parametric test i.e., Mann Whitney test. Bivariate correlation analysis was used for assessing significant correlation.

Results: The median (Inter Quartile Range) of semen ROS levels was 10.70 (86) RLU/sec/million sperm. Ten cases (40%) had elevated semen ROS levels (Reference level: 22RLU/sec/million sperm). The Median (IQR) value of sperm DFI was 31.84 % (13%). Seventeen cases had sperm DFI higher than reference value of 27%. Sperm DFI did not show significant correlation with semen oxidative stress (r = -0.033, p=0.88)

Conclusion: Semen reactive oxidative species and Sperm DNA fragmentation levels were not found to correlate with unexplained fetal malformations, although a trend towards higher levels was recognized in this pilot study. Thus, in couples having children with no identifiable cause of congenital malformation, implication of sperm molecular factors warrants further studies.

Keywords

Antioxidants, Congenital malformation, DNA fragmentation, Reactive oxygen species, Spermatozoa

How to cite this article :

Japleen Kaur, Dipika Deka, Rima Dada, Perumal Vanamail, Vatsla Dadhwal, Neena Malhotra, et al.. EVALUATION OF SEMEN OXIDATIVE STRESS AND SPERM DNA DAMAGE IN CASES OF UNEXPLAINED FOETAL CONGENITAL ANOMALIES- A PILOT STUDY. Journal of Clinical and Diagnostic Research [serial online] 2017 November [cited: 2018 Jan 17 ]; 11:QC01-QC04. Available from
http://www.jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=November&volume=11&issue=11&page=QC01-QC04&id=10830

DOI and Others

DOI: 10.7860/JCDR/2017/24714.10830

Date of Submission: Oct 09, 2016
Date of Peer Review: Jan 10, 2017
Date of Acceptance: Jul 20, 2017
Date of Publishing: Nov 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

JCDR is now Monthly and more widely Indexed .
  • Emerging Sources Citation Index (Web of Science, thomsonreuters)
  • Index Copernicus ICV 2016: 132.37
  • SCOPUS
  • Academic Search Complete Database
  • Directory of Open Access Journals (DOAJ)
  • EBSCOhost
  • Embase & EMbiology
  • Google Scholar
  • HINARI Access to Research in Health Programme
  • Indian Science Abstracts (ISA)
  • Journal seek Database
  • Google
  • Popline (reproductive health literature)
  • www.omnimedicalsearch.com