Activity of Catalase (CAT), ALT and AST in Different Organs of Swiss Albino Mice Treated with Lead Acetate, Vitamin C and Magnesium-L-ThreonateCorrespondence Address :
Dr. Ilir Nazmi Mazreku,
Str. Shaqir Igrishta, F2/12, Prishtina, Not Applicable, Albania.
Introduction: Lead is a natural element with toxic properties and is widespread in the environment. Lead toxicity is associated with generation of reactive oxygen and nitrogen species and consumption of antioxidants elements (vitamin E and C, glutathione, thioredoxin and lipoic acid, melatonin, carotenoids and natural flavonoids) in the cell, and unbalancing oxidants-antioxidants levels.
Aim: To evaluate the effects of different chemical combinations (lead acetate, Vitamin C and Magnesium-L-threonate) on antioxidant enzyme activity (catalase-CAT) of liver, kidney, spleen, pancreas and brain, and serum transaminases [Serum Alanine Transaminase (ALT) and Serum Aspartate Transaminase (AST)].
Materials and Methods: Experimental animals (49 male Mus musculus-swiss albino mice) were separated into five different groups. The first group was used as a control, hence the other four groups were treated with sub-lethal doses (90 mg/kg) of lead acetate (group 2), lead acetate (90 mg/kg) and Vitamin C dose 40mg/kg (group 3), lead acetate (90 mg/kg) and Magnesium-L-threonate dose 100 mg/kg (group 4) and only with Magnesium-L-threonate dose 100 mg/kg (group 5), during the treatment period (40 days). Blood samples were taken from the facial vein and used for transaminase analysis. Organ tissue was collected after euthanizing anaesthetized animals with neck dislocation technique.
Results: The results showed that lead acetate treatment has caused significant elevation in the activity of AST (group 2 and 3) and ALT (group 3). Also, CAT activity was significantly (p<0.05) increased in groups treated with lead acetate (liver, pancreas, kidney and brain but not in spleen). Treatment of lead intoxicated groups with Vitamin C and Magnesium L-threonate increased significantly CAT activity in brain.
Conclusion: Lead effects by interacting with different molecular systems and increasing enzyme activity (CAT, ALT and AST). Effects on CAT activity of Magnesium-L-threonate and Vitamin C treatment in lead acetate intoxication case are similar. Detoxifying properties of Vitamin C in the brain compared with other organs were very ineffective, because of Blood Brain Barrier (BBB) metabolic competences.
Antioxidants, Blood brain barrier, Dose, Toxicity
Ilir Nazmi Mazreku, Halil Ahmetaj, Valbona Aliko, Kemajl Bislimi, Fetah Halili, Jeton Halili. ACTIVITY OF CATALASE (CAT), ALT AND AST IN DIFFERENT ORGANS OF SWISS ALBINO MICE TREATED WITH LEAD ACETATE, VITAMIN C AND MAGNESIUM-L-THREONATE. Journal of Clinical and Diagnostic Research [serial online] 2017 November [cited: 2018 Jan 19 ]; 11:CC01-CC04. Available from
Date of Submission: May 22, 2017
Date of Peer Review: Jun 26, 2017
Date of Acceptance: Sep 25, 2017
Date of Publishing: Nov 01, 2017
FINANCIAL OR OTHER COMPETING INTERESTS: None.
- Emerging Sources Citation Index (Web of Science, thomsonreuters)
- Index Copernicus ICV 2016: 132.37
- Academic Search Complete Database
- Directory of Open Access Journals (DOAJ)
- Embase & EMbiology
- Google Scholar
- HINARI Access to Research in Health Programme
- Indian Science Abstracts (ISA)
- Journal seek Database
- Popline (reproductive health literature)